Staff View: Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease

Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease

The growth of adipose tissues is considered angiogenesis-dependent during non-alcoholic fatty liver disease (NAFLD). We have recently reported that our standardized 50% methanolic extract (ME) of Phyllanthus niruri (50% ME of P. niruri) has alleviated NAFLD in Sprague–Dawley rats. This study aimed t...

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Main Authors:	Al Zarzour, Raghdaa Hamdan, Alshawsh, Mohammed Abdullah, Asif, Muhammad, Al-Mansoub, Majed Ahmed, Mohamed, Zahurin, Ahmad, Mariam, Majid, Amin Malik Shah Abdul, Asmawi, Mohd Zaini, Kaur, Gurjeet, Al-Dualimi, Dhamraa Waleed, Mun, Fei Yam
Format:	Article
Published:	MDPI 2018
Subjects:	R Medicine
Online Access:	http://eprints.um.edu.my/22601/ https://doi.org/10.3390/nu10081057
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spelling	my.um.eprints.226012019-09-27T02:06:59Z http://eprints.um.edu.my/22601/ Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease Al Zarzour, Raghdaa Hamdan Alshawsh, Mohammed Abdullah Asif, Muhammad Al-Mansoub, Majed Ahmed Mohamed, Zahurin Ahmad, Mariam Majid, Amin Malik Shah Abdul Asmawi, Mohd Zaini Kaur, Gurjeet Al-Dualimi, Dhamraa Waleed Mun, Fei Yam R Medicine The growth of adipose tissues is considered angiogenesis-dependent during non-alcoholic fatty liver disease (NAFLD). We have recently reported that our standardized 50% methanolic extract (ME) of Phyllanthus niruri (50% ME of P. niruri) has alleviated NAFLD in Sprague–Dawley rats. This study aimed to assess the molecular mechanisms of action, and to further evaluate the antiangiogenic effect of this extract. NAFLD was induced by eight weeks of high-fat diet, and treatment was applied for four weeks. Antiangiogenic activity was assessed by aortic ring assay and by in vitro tests. Our findings demonstrated that the therapeutic effects of 50% ME among NAFLD rats, were associated with a significant increase in serum adiponectin, reduction in the serum levels of RBP4, vaspin, progranulin, TNF-α, IL-6, and significant downregulation of the hepatic gene expression of PPARγ, SLC10A2, and Collα1. Concomitantly, 50% ME of P. niruri has exhibited a potent antiangiogenic activity on ring assay, cell migration, vascular endothelial growth factor (VEGF), and tube formation, without any cytotoxic effect. Together, our findings revealed that the protective effects of P. niruri against NAFLD might be attributed to its antiangiogenic effect, as well as to the regulation of adipocytokines and reducing the expression of adipogenic genes. MDPI 2018 Article PeerReviewed Al Zarzour, Raghdaa Hamdan and Alshawsh, Mohammed Abdullah and Asif, Muhammad and Al-Mansoub, Majed Ahmed and Mohamed, Zahurin and Ahmad, Mariam and Majid, Amin Malik Shah Abdul and Asmawi, Mohd Zaini and Kaur, Gurjeet and Al-Dualimi, Dhamraa Waleed and Mun, Fei Yam (2018) Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease. Nutrients, 10 (8). p. 1057. ISSN 2072-6643 https://doi.org/10.3390/nu10081057 doi:10.3390/nu10081057
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine
spellingShingle	R Medicine Al Zarzour, Raghdaa Hamdan Alshawsh, Mohammed Abdullah Asif, Muhammad Al-Mansoub, Majed Ahmed Mohamed, Zahurin Ahmad, Mariam Majid, Amin Malik Shah Abdul Asmawi, Mohd Zaini Kaur, Gurjeet Al-Dualimi, Dhamraa Waleed Mun, Fei Yam Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
description	The growth of adipose tissues is considered angiogenesis-dependent during non-alcoholic fatty liver disease (NAFLD). We have recently reported that our standardized 50% methanolic extract (ME) of Phyllanthus niruri (50% ME of P. niruri) has alleviated NAFLD in Sprague–Dawley rats. This study aimed to assess the molecular mechanisms of action, and to further evaluate the antiangiogenic effect of this extract. NAFLD was induced by eight weeks of high-fat diet, and treatment was applied for four weeks. Antiangiogenic activity was assessed by aortic ring assay and by in vitro tests. Our findings demonstrated that the therapeutic effects of 50% ME among NAFLD rats, were associated with a significant increase in serum adiponectin, reduction in the serum levels of RBP4, vaspin, progranulin, TNF-α, IL-6, and significant downregulation of the hepatic gene expression of PPARγ, SLC10A2, and Collα1. Concomitantly, 50% ME of P. niruri has exhibited a potent antiangiogenic activity on ring assay, cell migration, vascular endothelial growth factor (VEGF), and tube formation, without any cytotoxic effect. Together, our findings revealed that the protective effects of P. niruri against NAFLD might be attributed to its antiangiogenic effect, as well as to the regulation of adipocytokines and reducing the expression of adipogenic genes.
format	Article
author	Al Zarzour, Raghdaa Hamdan Alshawsh, Mohammed Abdullah Asif, Muhammad Al-Mansoub, Majed Ahmed Mohamed, Zahurin Ahmad, Mariam Majid, Amin Malik Shah Abdul Asmawi, Mohd Zaini Kaur, Gurjeet Al-Dualimi, Dhamraa Waleed Mun, Fei Yam
author_facet	Al Zarzour, Raghdaa Hamdan Alshawsh, Mohammed Abdullah Asif, Muhammad Al-Mansoub, Majed Ahmed Mohamed, Zahurin Ahmad, Mariam Majid, Amin Malik Shah Abdul Asmawi, Mohd Zaini Kaur, Gurjeet Al-Dualimi, Dhamraa Waleed Mun, Fei Yam
author_sort	Al Zarzour, Raghdaa Hamdan
title	Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
title_short	Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
title_full	Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
title_fullStr	Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
title_full_unstemmed	Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease
title_sort	adipocytokine regulation and antiangiogenic activity underlie the molecular mechanisms of therapeutic effects of phyllanthus niruri against non-alcoholic fatty liver disease
publisher	MDPI
publishDate	2018
url	http://eprints.um.edu.my/22601/ https://doi.org/10.3390/nu10081057
_version_	1646210288123379712
score	13.211869

Adipocytokine Regulation and Antiangiogenic Activity Underlie the Molecular Mechanisms of Therapeutic Effects of Phyllanthus niruri against Non-Alcoholic Fatty Liver Disease

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