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Embryo culture conditions are significantly improved during uninterrupted incubation: A randomized controlled trial

A parallel group superiority prospective randomised controlled trial was devised to compare the culture characteristics of human pre-implantation stage embryos during uninterrupted culture in a time lapse incubator (TLI) versus the conventional model of interrupted culture in a standard incubator (S...

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Main Authors: Alhelou, Yousef, Mat Adenan, Noor Azmi, Ali, Jaffar
Format: Article
Published: Elsevier 2018
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/22568/
https://doi.org/10.1016/j.repbio.2017.12.003
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spelling my.um.eprints.225682019-09-26T02:17:08Z http://eprints.um.edu.my/22568/ Embryo culture conditions are significantly improved during uninterrupted incubation: A randomized controlled trial Alhelou, Yousef Mat Adenan, Noor Azmi Ali, Jaffar R Medicine A parallel group superiority prospective randomised controlled trial was devised to compare the culture characteristics of human pre-implantation stage embryos during uninterrupted culture in a time lapse incubator (TLI) versus the conventional model of interrupted culture in a standard incubator (SI) under low oxygen tension using a single step medium. 221 patients aged 35-and-under, 124 patients aged between 36 and 39 and 86 patients aged 40-and-over years were randomised and cultured either in a SI or in a TLI. Patients in the three age groups were distributed between the TLI and SI in a 1:1 ratio. The development of embryos on days 2, 3 and 5, and the clinical pregnancy and implantation rates were recorded. The fertilisation rate, development of day 2 and clinical pregnancy rates were similar in both treatments but the 8-cell development rate in all age groups combined (p = 0.016), blastocyst development rate (p = 0.0022) and the implantation rate (p = 0.0022) was significantly higher for the uninterrupted culture. These findings demonstrated significant differences between the two incubation groups. It also indicated less efficacious embryonic development with age in both treatments which appeared more pronounced in the conventional incubator. In conclusion uninterrupted culture is superior compared to the interrupted incubation culture system. Elsevier 2018 Article PeerReviewed Alhelou, Yousef and Mat Adenan, Noor Azmi and Ali, Jaffar (2018) Embryo culture conditions are significantly improved during uninterrupted incubation: A randomized controlled trial. Reproductive Biology, 18 (1). pp. 40-45. ISSN 1642-431X https://doi.org/10.1016/j.repbio.2017.12.003 doi:10.1016/j.repbio.2017.12.003
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Alhelou, Yousef
Mat Adenan, Noor Azmi
Ali, Jaffar
Embryo culture conditions are significantly improved during uninterrupted incubation: A randomized controlled trial
description A parallel group superiority prospective randomised controlled trial was devised to compare the culture characteristics of human pre-implantation stage embryos during uninterrupted culture in a time lapse incubator (TLI) versus the conventional model of interrupted culture in a standard incubator (SI) under low oxygen tension using a single step medium. 221 patients aged 35-and-under, 124 patients aged between 36 and 39 and 86 patients aged 40-and-over years were randomised and cultured either in a SI or in a TLI. Patients in the three age groups were distributed between the TLI and SI in a 1:1 ratio. The development of embryos on days 2, 3 and 5, and the clinical pregnancy and implantation rates were recorded. The fertilisation rate, development of day 2 and clinical pregnancy rates were similar in both treatments but the 8-cell development rate in all age groups combined (p = 0.016), blastocyst development rate (p = 0.0022) and the implantation rate (p = 0.0022) was significantly higher for the uninterrupted culture. These findings demonstrated significant differences between the two incubation groups. It also indicated less efficacious embryonic development with age in both treatments which appeared more pronounced in the conventional incubator. In conclusion uninterrupted culture is superior compared to the interrupted incubation culture system.
format Article
author Alhelou, Yousef
Mat Adenan, Noor Azmi
Ali, Jaffar
author_facet Alhelou, Yousef
Mat Adenan, Noor Azmi
Ali, Jaffar
author_sort Alhelou, Yousef
title Embryo culture conditions are significantly improved during uninterrupted incubation: A randomized controlled trial
title_short Embryo culture conditions are significantly improved during uninterrupted incubation: A randomized controlled trial
title_full Embryo culture conditions are significantly improved during uninterrupted incubation: A randomized controlled trial
title_fullStr Embryo culture conditions are significantly improved during uninterrupted incubation: A randomized controlled trial
title_full_unstemmed Embryo culture conditions are significantly improved during uninterrupted incubation: A randomized controlled trial
title_sort embryo culture conditions are significantly improved during uninterrupted incubation: a randomized controlled trial
publisher Elsevier
publishDate 2018
url http://eprints.um.edu.my/22568/
https://doi.org/10.1016/j.repbio.2017.12.003
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score 13.211869

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