Staff View: Occurrence of Acanthamoeba genotypes in Central West Malaysian environments

Occurrence of Acanthamoeba genotypes in Central West Malaysian environments

Acanthamoeba species are ubiquitous free-living protozoa that can be found worldwide. Occasionally, it can become parasitic and the causative agent of acanthamoebic keratitis (AK) and Granulomatous Amoebic Encephalitis (GAE) in man. A total of 160 environmental samples and 225 naturally-infected ani...

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Main Authors:	Basher, Mohamad Hafiz Abdul, Ithoi, Init, Mahmud, Rohela, Abdulsalam, Awatif Mohamed, Foead, Agus Iwan, Dawaki, Salwa, Atroosh, Wahib Mohammed Mohsen, Nissapatorn, Veeranoot, Abdullah, Wan Omar
Format:	Article
Published:	Elsevier 2018
Subjects:	R Medicine
Online Access:	http://eprints.um.edu.my/22346/ https://doi.org/10.1016/j.actatropica.2017.11.015
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spelling	my.um.eprints.223462019-09-13T07:01:07Z http://eprints.um.edu.my/22346/ Occurrence of Acanthamoeba genotypes in Central West Malaysian environments Basher, Mohamad Hafiz Abdul Ithoi, Init Mahmud, Rohela Abdulsalam, Awatif Mohamed Foead, Agus Iwan Dawaki, Salwa Atroosh, Wahib Mohammed Mohsen Nissapatorn, Veeranoot Abdullah, Wan Omar R Medicine Acanthamoeba species are ubiquitous free-living protozoa that can be found worldwide. Occasionally, it can become parasitic and the causative agent of acanthamoebic keratitis (AK) and Granulomatous Amoebic Encephalitis (GAE) in man. A total of 160 environmental samples and 225 naturally-infected animal corneal swabs were collected for Acanthamoeba cultivation. Acanthamoeba was found to be high in samples collected from environments (85%, 136/160) compared to infected animal corneas (24.89%, 56/225) by microscopic examination. Analysis of nucleotide sequence of 18S rRNA gene of all the 192 cultivable Acanthamoeba isolates revealed 4 genotypes (T3, T4. T5 and T15) with T4 as the most prevalent (69.27%, 133/192) followed by T5 (20.31%), T15 (9.90%) and T3 (0.52%). Genotype T4 was from the strain of A. castellanii U07401 (44.27%), A. castellanii U07409 (20.83%) and A. polyphagaAY026243 (4.17%), but interestingly, only A. castellanii U07401 was detected in naturally infected corneal samples. In environmental samples, T4 was commonly detected in all samples including dry soil, dust, wet debris, wet soil and water. Among the T4, A. castellanii (U07409) strains were detected high occurrence in dry (45%) followed by aquatic (32.50%) and moist (22.50%) samples but however A. castellanii (U07401) strains were dominant in dry samples of soil and dust (93.10%). Subsequently, genotype T5 of A. lenticulata (U94741) strains were dominant in samples collected from aquatic environments (58.97%). In summary, A. castellanii (U07401) strains were found dominant in both environmental and corneal swab samples. Therefore, these strains are possibly the most virulent and dry soil or dusts are the most possible source of Acanthamoeba infection in cats and dogs corneas. Elsevier 2018 Article PeerReviewed Basher, Mohamad Hafiz Abdul and Ithoi, Init and Mahmud, Rohela and Abdulsalam, Awatif Mohamed and Foead, Agus Iwan and Dawaki, Salwa and Atroosh, Wahib Mohammed Mohsen and Nissapatorn, Veeranoot and Abdullah, Wan Omar (2018) Occurrence of Acanthamoeba genotypes in Central West Malaysian environments. Acta Tropica, 178. pp. 219-228. ISSN 0001-706X https://doi.org/10.1016/j.actatropica.2017.11.015 doi:10.1016/j.actatropica.2017.11.015
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine
spellingShingle	R Medicine Basher, Mohamad Hafiz Abdul Ithoi, Init Mahmud, Rohela Abdulsalam, Awatif Mohamed Foead, Agus Iwan Dawaki, Salwa Atroosh, Wahib Mohammed Mohsen Nissapatorn, Veeranoot Abdullah, Wan Omar Occurrence of Acanthamoeba genotypes in Central West Malaysian environments
description	Acanthamoeba species are ubiquitous free-living protozoa that can be found worldwide. Occasionally, it can become parasitic and the causative agent of acanthamoebic keratitis (AK) and Granulomatous Amoebic Encephalitis (GAE) in man. A total of 160 environmental samples and 225 naturally-infected animal corneal swabs were collected for Acanthamoeba cultivation. Acanthamoeba was found to be high in samples collected from environments (85%, 136/160) compared to infected animal corneas (24.89%, 56/225) by microscopic examination. Analysis of nucleotide sequence of 18S rRNA gene of all the 192 cultivable Acanthamoeba isolates revealed 4 genotypes (T3, T4. T5 and T15) with T4 as the most prevalent (69.27%, 133/192) followed by T5 (20.31%), T15 (9.90%) and T3 (0.52%). Genotype T4 was from the strain of A. castellanii U07401 (44.27%), A. castellanii U07409 (20.83%) and A. polyphagaAY026243 (4.17%), but interestingly, only A. castellanii U07401 was detected in naturally infected corneal samples. In environmental samples, T4 was commonly detected in all samples including dry soil, dust, wet debris, wet soil and water. Among the T4, A. castellanii (U07409) strains were detected high occurrence in dry (45%) followed by aquatic (32.50%) and moist (22.50%) samples but however A. castellanii (U07401) strains were dominant in dry samples of soil and dust (93.10%). Subsequently, genotype T5 of A. lenticulata (U94741) strains were dominant in samples collected from aquatic environments (58.97%). In summary, A. castellanii (U07401) strains were found dominant in both environmental and corneal swab samples. Therefore, these strains are possibly the most virulent and dry soil or dusts are the most possible source of Acanthamoeba infection in cats and dogs corneas.
format	Article
author	Basher, Mohamad Hafiz Abdul Ithoi, Init Mahmud, Rohela Abdulsalam, Awatif Mohamed Foead, Agus Iwan Dawaki, Salwa Atroosh, Wahib Mohammed Mohsen Nissapatorn, Veeranoot Abdullah, Wan Omar
author_facet	Basher, Mohamad Hafiz Abdul Ithoi, Init Mahmud, Rohela Abdulsalam, Awatif Mohamed Foead, Agus Iwan Dawaki, Salwa Atroosh, Wahib Mohammed Mohsen Nissapatorn, Veeranoot Abdullah, Wan Omar
author_sort	Basher, Mohamad Hafiz Abdul
title	Occurrence of Acanthamoeba genotypes in Central West Malaysian environments
title_short	Occurrence of Acanthamoeba genotypes in Central West Malaysian environments
title_full	Occurrence of Acanthamoeba genotypes in Central West Malaysian environments
title_fullStr	Occurrence of Acanthamoeba genotypes in Central West Malaysian environments
title_full_unstemmed	Occurrence of Acanthamoeba genotypes in Central West Malaysian environments
title_sort	occurrence of acanthamoeba genotypes in central west malaysian environments
publisher	Elsevier
publishDate	2018
url	http://eprints.um.edu.my/22346/ https://doi.org/10.1016/j.actatropica.2017.11.015
_version_	1646210215168704512
score	13.211869

Occurrence of Acanthamoeba genotypes in Central West Malaysian environments

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