Staff View: Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis

Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis

Objectives: The serological diagnosis of human infection with Angiostrongylus cantonensis remains problematic because there are no commercially available validated tests. Most laboratories use domestically prepared tests such as the enzyme-linked immunosorbent assay (ELISA) or immunoblotting. Since...

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Main Authors:	Eamsobhana, Praphathip, Tungtrongchitr, Anchalee, Wanachiwanawin, Darawan, Yong, Hoi Sen
Format:	Article
Published:	Elsevier 2018
Subjects:	Q Science (General) QH Natural history
Online Access:	http://eprints.um.edu.my/22115/ https://doi.org/10.1016/j.ijid.2018.06.005
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spelling	my.um.eprints.221152019-12-04T08:50:46Z http://eprints.um.edu.my/22115/ Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis Eamsobhana, Praphathip Tungtrongchitr, Anchalee Wanachiwanawin, Darawan Yong, Hoi Sen Q Science (General) QH Natural history Objectives: The serological diagnosis of human infection with Angiostrongylus cantonensis remains problematic because there are no commercially available validated tests. Most laboratories use domestically prepared tests such as the enzyme-linked immunosorbent assay (ELISA) or immunoblotting. Since laboratory facilities are not always available in endemic areas, we developed and assessed a rapid lateral flow immunochromatographic assay (AcQuick Dx Test) to detect anti-A. cantonensis antibodies in human serum. Methods: The test device was assembled with purified 31-kDa glycoprotein as diagnostic antigen and with gold-labelled anti-human immunoglublin-G as the detector reagent. A total of 97 serum samples were tested – 19 samples from clinically diagnosed patients with detectable A. cantonensis-specific antibody in immunoblotting; 43 samples from patients with other parasitic diseases, i.e. gnathostomiasis (n = 13), toxocariasis (n = 2), trichinellosis (n = 2), hookworm infection (n = 4), filariasis (n = 5), cysticercosis (n = 9), paragonimiasis (n = 2), opisthorchiasis (n = 3), and malaria (n = 3); and 35 samples from normal healthy subjects. Results: The sensitivity, specificity, positive predictive value and negative predictive value of AcQuick Dx Test to detect anti-A. cantonensis specific antibodies in serologically confirmed angiostrongyliasis cases, were 100%, 98.72%, 95% and 100%, respectively. Positive AcQuick Dx was observed in 1 of 4 cases with hookworm infections. No positive AcQuick Dx was observed in cases with other parasitic diseases, and the individual healthy subjects. Conclusions: AcQuick Dx Test is rapid, highly sensitive and specific, and easy to perform without additional equipment or ancillary supplies. It yields results that are interpreted visually, and possesses a long shelf-life at room temperature. Thus, it can be applied as an additional test for clinical diagnostic support of angiostrongyliasis either in conventional laboratories or for remote areas where laboratory infrastructure is not available. Elsevier 2018 Article PeerReviewed Eamsobhana, Praphathip and Tungtrongchitr, Anchalee and Wanachiwanawin, Darawan and Yong, Hoi Sen (2018) Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis. International Journal of Infectious Diseases, 73. pp. 69-71. ISSN 1201-9712 https://doi.org/10.1016/j.ijid.2018.06.005 doi:10.1016/j.ijid.2018.06.005
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QH Natural history
spellingShingle	Q Science (General) QH Natural history Eamsobhana, Praphathip Tungtrongchitr, Anchalee Wanachiwanawin, Darawan Yong, Hoi Sen Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis
description	Objectives: The serological diagnosis of human infection with Angiostrongylus cantonensis remains problematic because there are no commercially available validated tests. Most laboratories use domestically prepared tests such as the enzyme-linked immunosorbent assay (ELISA) or immunoblotting. Since laboratory facilities are not always available in endemic areas, we developed and assessed a rapid lateral flow immunochromatographic assay (AcQuick Dx Test) to detect anti-A. cantonensis antibodies in human serum. Methods: The test device was assembled with purified 31-kDa glycoprotein as diagnostic antigen and with gold-labelled anti-human immunoglublin-G as the detector reagent. A total of 97 serum samples were tested – 19 samples from clinically diagnosed patients with detectable A. cantonensis-specific antibody in immunoblotting; 43 samples from patients with other parasitic diseases, i.e. gnathostomiasis (n = 13), toxocariasis (n = 2), trichinellosis (n = 2), hookworm infection (n = 4), filariasis (n = 5), cysticercosis (n = 9), paragonimiasis (n = 2), opisthorchiasis (n = 3), and malaria (n = 3); and 35 samples from normal healthy subjects. Results: The sensitivity, specificity, positive predictive value and negative predictive value of AcQuick Dx Test to detect anti-A. cantonensis specific antibodies in serologically confirmed angiostrongyliasis cases, were 100%, 98.72%, 95% and 100%, respectively. Positive AcQuick Dx was observed in 1 of 4 cases with hookworm infections. No positive AcQuick Dx was observed in cases with other parasitic diseases, and the individual healthy subjects. Conclusions: AcQuick Dx Test is rapid, highly sensitive and specific, and easy to perform without additional equipment or ancillary supplies. It yields results that are interpreted visually, and possesses a long shelf-life at room temperature. Thus, it can be applied as an additional test for clinical diagnostic support of angiostrongyliasis either in conventional laboratories or for remote areas where laboratory infrastructure is not available.
format	Article
author	Eamsobhana, Praphathip Tungtrongchitr, Anchalee Wanachiwanawin, Darawan Yong, Hoi Sen
author_facet	Eamsobhana, Praphathip Tungtrongchitr, Anchalee Wanachiwanawin, Darawan Yong, Hoi Sen
author_sort	Eamsobhana, Praphathip
title	Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis
title_short	Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis
title_full	Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis
title_fullStr	Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis
title_full_unstemmed	Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis
title_sort	immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis
publisher	Elsevier
publishDate	2018
url	http://eprints.um.edu.my/22115/ https://doi.org/10.1016/j.ijid.2018.06.005
_version_	1654960696286248960
score	13.211869

Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis

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