Cover Image

Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia

Microsporidia are obligate intracellular parasitic fungi causing chronic diarrhea, particularly among immunocompromised patients. The current method used for diagnosis is based on conventional microscopy, which does not differentiate parasites at the species level. The present study was carried out...

Full description

Saved in:
Bibliographic Details
Main Authors: Hassan, Nur-Amirah, Lim, Yvonne Ai Lian, Mahmud, Rohela, Mohd-Shaharuddin, Norashikin, Wan Sulaiman, Wan Yusoff, Ngui, Romano
Format: Article
Published: American Society of Tropical Medicine and Hygiene 2018
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/21911/
https://doi.org/10.4269/ajtmh.17-0901
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.21911
record_format eprints
spelling my.um.eprints.219112019-08-08T06:53:53Z http://eprints.um.edu.my/21911/ Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia Hassan, Nur-Amirah Lim, Yvonne Ai Lian Mahmud, Rohela Mohd-Shaharuddin, Norashikin Wan Sulaiman, Wan Yusoff Ngui, Romano R Medicine Microsporidia are obligate intracellular parasitic fungi causing chronic diarrhea, particularly among immunocompromised patients. The current method used for diagnosis is based on conventional microscopy, which does not differentiate parasites at the species level. The present study was carried out to identify microsporidian species in immunocompromised patients. From March 2016 to March 2017, a total of 289 archived stool samples were examined microscopically for microsporidian spores using Gram-chromotrope Kinyoun (GCK) stain. Positive stool samples by microscopy were subjected to polymerase chain reaction and sequencing for species identification. Based on microscopy examination, the overall prevalence of microsporidian infections was 32.2% (93/289; 95% CI = 27.1–37.8). Of these stool samples, 45 samples were successfully amplified and confirmed as Enterocytozoon bieneusi. No Encephalitozoon intestinalis was detected. Accurate identification of species might help clinicians to decide appropriate management strategies as dissemination risks and treatment response vary for different species, hence improving the management of microsporidian infections. American Society of Tropical Medicine and Hygiene 2018 Article PeerReviewed Hassan, Nur-Amirah and Lim, Yvonne Ai Lian and Mahmud, Rohela and Mohd-Shaharuddin, Norashikin and Wan Sulaiman, Wan Yusoff and Ngui, Romano (2018) Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia. The American Journal of Tropical Medicine and Hygiene, 99 (6). pp. 1562-1566. ISSN 0002-9637 https://doi.org/10.4269/ajtmh.17-0901 doi:10.4269/ajtmh.17-0901
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Hassan, Nur-Amirah
Lim, Yvonne Ai Lian
Mahmud, Rohela
Mohd-Shaharuddin, Norashikin
Wan Sulaiman, Wan Yusoff
Ngui, Romano
Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia
description Microsporidia are obligate intracellular parasitic fungi causing chronic diarrhea, particularly among immunocompromised patients. The current method used for diagnosis is based on conventional microscopy, which does not differentiate parasites at the species level. The present study was carried out to identify microsporidian species in immunocompromised patients. From March 2016 to March 2017, a total of 289 archived stool samples were examined microscopically for microsporidian spores using Gram-chromotrope Kinyoun (GCK) stain. Positive stool samples by microscopy were subjected to polymerase chain reaction and sequencing for species identification. Based on microscopy examination, the overall prevalence of microsporidian infections was 32.2% (93/289; 95% CI = 27.1–37.8). Of these stool samples, 45 samples were successfully amplified and confirmed as Enterocytozoon bieneusi. No Encephalitozoon intestinalis was detected. Accurate identification of species might help clinicians to decide appropriate management strategies as dissemination risks and treatment response vary for different species, hence improving the management of microsporidian infections.
format Article
author Hassan, Nur-Amirah
Lim, Yvonne Ai Lian
Mahmud, Rohela
Mohd-Shaharuddin, Norashikin
Wan Sulaiman, Wan Yusoff
Ngui, Romano
author_facet Hassan, Nur-Amirah
Lim, Yvonne Ai Lian
Mahmud, Rohela
Mohd-Shaharuddin, Norashikin
Wan Sulaiman, Wan Yusoff
Ngui, Romano
author_sort Hassan, Nur-Amirah
title Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia
title_short Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia
title_full Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia
title_fullStr Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia
title_full_unstemmed Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia
title_sort molecular diagnosis of microsporidia among immunocompromised patients in kuala lumpur, malaysia
publisher American Society of Tropical Medicine and Hygiene
publishDate 2018
url http://eprints.um.edu.my/21911/
https://doi.org/10.4269/ajtmh.17-0901
_version_ 1643691696398008320
score 13.211869

Similar Items