Staff View: Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways

Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways

The aim of the present study is to isolate bioactive compounds from the roots of Piper sarmentosum and examine the mechanism of action using human breast cancer cell line (MDA-MB-231). Bioassay guided-fractionation of methanolic extract led to the isolation of asaricin (1) and isoasarone (2). Asaric...

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Main Authors:	Hematpoor, Arshia, Paydar, Mohammadjavad, Liew, Sook Yee, Sivasothy, Yasodha, Mohebali, Nooshin, Looi, Chung Yeng, Wong, Won Fen, Sofian-Azirun, Mohd, Awang, Khalijah
Format:	Article
Published:	Elsevier 2018
Subjects:	QD Chemistry QH Natural history R Medicine
Online Access:	http://eprints.um.edu.my/21906/ https://doi.org/10.1016/j.cbi.2017.11.014
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spelling	my.um.eprints.219062019-08-08T06:03:06Z http://eprints.um.edu.my/21906/ Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways Hematpoor, Arshia Paydar, Mohammadjavad Liew, Sook Yee Sivasothy, Yasodha Mohebali, Nooshin Looi, Chung Yeng Wong, Won Fen Sofian-Azirun, Mohd Awang, Khalijah QD Chemistry QH Natural history R Medicine The aim of the present study is to isolate bioactive compounds from the roots of Piper sarmentosum and examine the mechanism of action using human breast cancer cell line (MDA-MB-231). Bioassay guided-fractionation of methanolic extract led to the isolation of asaricin (1) and isoasarone (2). Asaricin (1) and isoasarone (2) had significant cytotoxicity towards MDA-MB-231. MCF-10A (human normal breast epithelial cells) cells are less sensitive than MDA-MB-231, but they respond to the treatment with the same unit of measurement. Both compounds increase reactive oxygen species (ROS), decrease mitochondrial membrane potential (MMP) and enhance cytochrome c release in treated MDA-MB-231 cells. Isoasarone (2) markedly elevated caspase -8 and -3/7 activities and caused a decline in nuclear NF-κB translocation, suggesting extrinsic, death receptor-linked apoptosis pathway. Quantitative PCR results of MDA-MB-231 treated with asaricin (1) and isoasarone (2) showed altered expression of Bcl-2: Bax level. The inhibitory potency of these isolates may support the therapeutic uses of these compounds in breast cancer. Elsevier 2018 Article PeerReviewed Hematpoor, Arshia and Paydar, Mohammadjavad and Liew, Sook Yee and Sivasothy, Yasodha and Mohebali, Nooshin and Looi, Chung Yeng and Wong, Won Fen and Sofian-Azirun, Mohd and Awang, Khalijah (2018) Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways. Chemico-Biological Interactions, 279. pp. 210-218. ISSN 0009-2797 https://doi.org/10.1016/j.cbi.2017.11.014 doi:10.1016/j.cbi.2017.11.014
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	QD Chemistry QH Natural history R Medicine
spellingShingle	QD Chemistry QH Natural history R Medicine Hematpoor, Arshia Paydar, Mohammadjavad Liew, Sook Yee Sivasothy, Yasodha Mohebali, Nooshin Looi, Chung Yeng Wong, Won Fen Sofian-Azirun, Mohd Awang, Khalijah Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways
description	The aim of the present study is to isolate bioactive compounds from the roots of Piper sarmentosum and examine the mechanism of action using human breast cancer cell line (MDA-MB-231). Bioassay guided-fractionation of methanolic extract led to the isolation of asaricin (1) and isoasarone (2). Asaricin (1) and isoasarone (2) had significant cytotoxicity towards MDA-MB-231. MCF-10A (human normal breast epithelial cells) cells are less sensitive than MDA-MB-231, but they respond to the treatment with the same unit of measurement. Both compounds increase reactive oxygen species (ROS), decrease mitochondrial membrane potential (MMP) and enhance cytochrome c release in treated MDA-MB-231 cells. Isoasarone (2) markedly elevated caspase -8 and -3/7 activities and caused a decline in nuclear NF-κB translocation, suggesting extrinsic, death receptor-linked apoptosis pathway. Quantitative PCR results of MDA-MB-231 treated with asaricin (1) and isoasarone (2) showed altered expression of Bcl-2: Bax level. The inhibitory potency of these isolates may support the therapeutic uses of these compounds in breast cancer.
format	Article
author	Hematpoor, Arshia Paydar, Mohammadjavad Liew, Sook Yee Sivasothy, Yasodha Mohebali, Nooshin Looi, Chung Yeng Wong, Won Fen Sofian-Azirun, Mohd Awang, Khalijah
author_facet	Hematpoor, Arshia Paydar, Mohammadjavad Liew, Sook Yee Sivasothy, Yasodha Mohebali, Nooshin Looi, Chung Yeng Wong, Won Fen Sofian-Azirun, Mohd Awang, Khalijah
author_sort	Hematpoor, Arshia
title	Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways
title_short	Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways
title_full	Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways
title_fullStr	Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways
title_full_unstemmed	Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways
title_sort	phenylpropanoids isolated from piper sarmentosum roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways
publisher	Elsevier
publishDate	2018
url	http://eprints.um.edu.my/21906/ https://doi.org/10.1016/j.cbi.2017.11.014
_version_	1643691694292467712
score	13.211869

Phenylpropanoids isolated from Piper sarmentosum Roxb. induce apoptosis in breast cancer cells through reactive oxygen species and mitochondrial-dependent pathways

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