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Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi

The aim of this study was to develop a recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip method for specific diagnosis of Plasmodium knowlesi. With incubation at 37C, the 18S rRNA gene of P. knowlesi was successfully amplified within 12 minutes. By adding a specifica...

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Main Authors: Lai, Meng Yee, Ooi, Choo Huck, Lau, Yee Ling
Format: Article
Published: American Society of Tropical Medicine and Hygiene 2018
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/21561/
https://doi.org/10.4269/ajtmh.17-0738
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spelling my.um.eprints.215612019-06-27T03:41:59Z http://eprints.um.edu.my/21561/ Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi Lai, Meng Yee Ooi, Choo Huck Lau, Yee Ling R Medicine The aim of this study was to develop a recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip method for specific diagnosis of Plasmodium knowlesi. With incubation at 37C, the 18S rRNA gene of P. knowlesi was successfully amplified within 12 minutes. By adding a specifically designed probe to the reaction solution, the amplified RPA product can be visualized on a LF strip. The RPA assay exhibited high sensitivity with limits of detection down to 10 parasites/μL of P. knowlesi. Nonetheless, it was demonstrated that all P. knowlesi (N = 41) and other Plasmodium sp. (N = 25) were positive while negative samples (N = 8) were negative. Therefore, a combination of RPA and LF strip detection is a highly promising approach with the potential to be suitable for use in resource-limited settings. American Society of Tropical Medicine and Hygiene 2018 Article PeerReviewed Lai, Meng Yee and Ooi, Choo Huck and Lau, Yee Ling (2018) Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi. The American Journal of Tropical Medicine and Hygiene, 98 (3). pp. 700-703. ISSN 0002-9637 https://doi.org/10.4269/ajtmh.17-0738 doi:10.4269/ajtmh.17-0738
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Lai, Meng Yee
Ooi, Choo Huck
Lau, Yee Ling
Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi
description The aim of this study was to develop a recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip method for specific diagnosis of Plasmodium knowlesi. With incubation at 37C, the 18S rRNA gene of P. knowlesi was successfully amplified within 12 minutes. By adding a specifically designed probe to the reaction solution, the amplified RPA product can be visualized on a LF strip. The RPA assay exhibited high sensitivity with limits of detection down to 10 parasites/μL of P. knowlesi. Nonetheless, it was demonstrated that all P. knowlesi (N = 41) and other Plasmodium sp. (N = 25) were positive while negative samples (N = 8) were negative. Therefore, a combination of RPA and LF strip detection is a highly promising approach with the potential to be suitable for use in resource-limited settings.
format Article
author Lai, Meng Yee
Ooi, Choo Huck
Lau, Yee Ling
author_facet Lai, Meng Yee
Ooi, Choo Huck
Lau, Yee Ling
author_sort Lai, Meng Yee
title Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi
title_short Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi
title_full Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi
title_fullStr Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi
title_full_unstemmed Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi
title_sort recombinase polymerase amplification combined with a lateral flow strip for the detection of plasmodium knowlesi
publisher American Society of Tropical Medicine and Hygiene
publishDate 2018
url http://eprints.um.edu.my/21561/
https://doi.org/10.4269/ajtmh.17-0738
_version_ 1643691594785751040
score 13.211869

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