Cover Image

Application of Two-Level Full Factorial Design for the Extraction of Fucoxanthin and Antioxidant Activities from Sargassum siliquosum and Sargassum polycystum

Two-level full factorial design was employed to identify the extraction parameters that can improve the derivation of fucoxanthin content (FC), total carotenoid content (TCC), and antioxidant from two brown seaweeds, Sargassum siliquosum (SS) and Sargassum polycystum (SP). These parameters included...

Full description

Saved in:
Bibliographic Details
Main Authors: Lim, Michelle Wen San, Tan, Kar Mem, Chew, Lye Yee, Kong, Kin Weng, Yan, See Wan
Format: Article
Published: Taylor & Francis 2018
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/21439/
https://doi.org/10.1080/10498850.2018.1448918
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.21439
record_format eprints
spelling my.um.eprints.214392019-05-31T03:33:15Z http://eprints.um.edu.my/21439/ Application of Two-Level Full Factorial Design for the Extraction of Fucoxanthin and Antioxidant Activities from Sargassum siliquosum and Sargassum polycystum Lim, Michelle Wen San Tan, Kar Mem Chew, Lye Yee Kong, Kin Weng Yan, See Wan R Medicine Two-level full factorial design was employed to identify the extraction parameters that can improve the derivation of fucoxanthin content (FC), total carotenoid content (TCC), and antioxidant from two brown seaweeds, Sargassum siliquosum (SS) and Sargassum polycystum (SP). These parameters included temperature (A: 4–45°C), time (B: 30–1,440 min), and solvent-to-solid ratio (C: 10–50 ml/g). Antioxidant activities were determined as trolox equivalent antioxidant capacity (TEAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, and ferric-reducing antioxidant power (FRAP). Results showed that all three factors were significant (p < 0.05) in providing higher FC in both species. These factors were also significant in obtaining higher TCC in SS; whereas in SP, TCC was only affected by solvent-to-solid ratio. Only temperature was found to contribute significantly to a higher TEAC in both species. However, none of the factors improved DPPH for SS, except temperature and time for SP. For SS, only time was significant in obtaining higher FRAP; whereas temperature and time were significant for SP. Hence, results indicate that a simple modification in the extraction temperature, time, and solvent-to-solid ratio will be able to improve the derivation of fucoxanthin, carotenoids, and antioxidant activities. Taylor & Francis 2018 Article PeerReviewed Lim, Michelle Wen San and Tan, Kar Mem and Chew, Lye Yee and Kong, Kin Weng and Yan, See Wan (2018) Application of Two-Level Full Factorial Design for the Extraction of Fucoxanthin and Antioxidant Activities from Sargassum siliquosum and Sargassum polycystum. Journal of Aquatic Food Product Technology, 27 (4). pp. 446-463. ISSN 1049-8850 https://doi.org/10.1080/10498850.2018.1448918 doi:10.1080/10498850.2018.1448918
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Lim, Michelle Wen San
Tan, Kar Mem
Chew, Lye Yee
Kong, Kin Weng
Yan, See Wan
Application of Two-Level Full Factorial Design for the Extraction of Fucoxanthin and Antioxidant Activities from Sargassum siliquosum and Sargassum polycystum
description Two-level full factorial design was employed to identify the extraction parameters that can improve the derivation of fucoxanthin content (FC), total carotenoid content (TCC), and antioxidant from two brown seaweeds, Sargassum siliquosum (SS) and Sargassum polycystum (SP). These parameters included temperature (A: 4–45°C), time (B: 30–1,440 min), and solvent-to-solid ratio (C: 10–50 ml/g). Antioxidant activities were determined as trolox equivalent antioxidant capacity (TEAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, and ferric-reducing antioxidant power (FRAP). Results showed that all three factors were significant (p < 0.05) in providing higher FC in both species. These factors were also significant in obtaining higher TCC in SS; whereas in SP, TCC was only affected by solvent-to-solid ratio. Only temperature was found to contribute significantly to a higher TEAC in both species. However, none of the factors improved DPPH for SS, except temperature and time for SP. For SS, only time was significant in obtaining higher FRAP; whereas temperature and time were significant for SP. Hence, results indicate that a simple modification in the extraction temperature, time, and solvent-to-solid ratio will be able to improve the derivation of fucoxanthin, carotenoids, and antioxidant activities.
format Article
author Lim, Michelle Wen San
Tan, Kar Mem
Chew, Lye Yee
Kong, Kin Weng
Yan, See Wan
author_facet Lim, Michelle Wen San
Tan, Kar Mem
Chew, Lye Yee
Kong, Kin Weng
Yan, See Wan
author_sort Lim, Michelle Wen San
title Application of Two-Level Full Factorial Design for the Extraction of Fucoxanthin and Antioxidant Activities from Sargassum siliquosum and Sargassum polycystum
title_short Application of Two-Level Full Factorial Design for the Extraction of Fucoxanthin and Antioxidant Activities from Sargassum siliquosum and Sargassum polycystum
title_full Application of Two-Level Full Factorial Design for the Extraction of Fucoxanthin and Antioxidant Activities from Sargassum siliquosum and Sargassum polycystum
title_fullStr Application of Two-Level Full Factorial Design for the Extraction of Fucoxanthin and Antioxidant Activities from Sargassum siliquosum and Sargassum polycystum
title_full_unstemmed Application of Two-Level Full Factorial Design for the Extraction of Fucoxanthin and Antioxidant Activities from Sargassum siliquosum and Sargassum polycystum
title_sort application of two-level full factorial design for the extraction of fucoxanthin and antioxidant activities from sargassum siliquosum and sargassum polycystum
publisher Taylor & Francis
publishDate 2018
url http://eprints.um.edu.my/21439/
https://doi.org/10.1080/10498850.2018.1448918
_version_ 1643691564869877760
score 13.211869

Similar Items