Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria
The present study aims to develop a method for rapid diagnosis of malaria using loop-mediated isothermal amplification (LAMP) combined with a lateral flow device (LFD). By adding the biotin-labeled and fluorescein amiditelabeled loop primers to the LAMP reaction solution, the end product can be visu...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Published: |
American Society of Tropical Medicine and Hygiene
2018
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/21352/ https://doi.org/10.4269/ajtmh.18-0177 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my.um.eprints.21352 |
|---|---|
| record_format |
eprints |
| spelling |
my.um.eprints.213522019-05-28T03:07:51Z http://eprints.um.edu.my/21352/ Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria Mallepaddi, Prudhvi Chand Lai, Meng Yee Podha, Sudhakar Ooi, Choo Huck Liew, Jonathan Wee Kent Polavarapu, Rathnagiri Lau, Yee Ling R Medicine The present study aims to develop a method for rapid diagnosis of malaria using loop-mediated isothermal amplification (LAMP) combined with a lateral flow device (LFD). By adding the biotin-labeled and fluorescein amiditelabeled loop primers to the LAMP reaction solution, the end product can be visualized on a LFD. The entire procedure takes approximately 42 minutes tocomplete, LAMP assay exhibited high sensitivity, asthe detection limit was 0.01 pg/μL for all five Plasmodium species. Itwas demonstrated that all Plasmodium knowlesi (N = 90) and Plasmodium vivax(N= 56) were positively amplified by LAMP-LFD assay, whereas healthy donor samples (N = 8) were negative. However, not all mixed infections were positive, and other infected nonmalaria samples were negative. Loop-mediated isothermal amplification-LFD representsarobust approach with potential suitability for useinresource-constrained laboratories. We believe that LAMP-LFD has a potential to be developed as point-of-care diagnostic tool in future. American Society of Tropical Medicine and Hygiene 2018 Article PeerReviewed Mallepaddi, Prudhvi Chand and Lai, Meng Yee and Podha, Sudhakar and Ooi, Choo Huck and Liew, Jonathan Wee Kent and Polavarapu, Rathnagiri and Lau, Yee Ling (2018) Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria. The American Journal of Tropical Medicine and Hygiene, 99 (3). pp. 704-708. ISSN 0002-9637 https://doi.org/10.4269/ajtmh.18-0177 doi:10.4269/ajtmh.18-0177 |
| institution |
Universiti Malaya |
| building |
UM Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Malaya |
| content_source |
UM Research Repository |
| url_provider |
http://eprints.um.edu.my/ |
| topic |
R Medicine |
| spellingShingle |
R Medicine Mallepaddi, Prudhvi Chand Lai, Meng Yee Podha, Sudhakar Ooi, Choo Huck Liew, Jonathan Wee Kent Polavarapu, Rathnagiri Lau, Yee Ling Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria |
| description |
The present study aims to develop a method for rapid diagnosis of malaria using loop-mediated isothermal amplification (LAMP) combined with a lateral flow device (LFD). By adding the biotin-labeled and fluorescein amiditelabeled loop primers to the LAMP reaction solution, the end product can be visualized on a LFD. The entire procedure takes approximately 42 minutes tocomplete, LAMP assay exhibited high sensitivity, asthe detection limit was 0.01 pg/μL for all five Plasmodium species. Itwas demonstrated that all Plasmodium knowlesi (N = 90) and Plasmodium vivax(N= 56) were positively amplified by LAMP-LFD assay, whereas healthy donor samples (N = 8) were negative. However, not all mixed infections were positive, and other infected nonmalaria samples were negative. Loop-mediated isothermal amplification-LFD representsarobust approach with potential suitability for useinresource-constrained laboratories. We believe that LAMP-LFD has a potential to be developed as point-of-care diagnostic tool in future. |
| format |
Article |
| author |
Mallepaddi, Prudhvi Chand Lai, Meng Yee Podha, Sudhakar Ooi, Choo Huck Liew, Jonathan Wee Kent Polavarapu, Rathnagiri Lau, Yee Ling |
| author_facet |
Mallepaddi, Prudhvi Chand Lai, Meng Yee Podha, Sudhakar Ooi, Choo Huck Liew, Jonathan Wee Kent Polavarapu, Rathnagiri Lau, Yee Ling |
| author_sort |
Mallepaddi, Prudhvi Chand |
| title |
Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria |
| title_short |
Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria |
| title_full |
Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria |
| title_fullStr |
Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria |
| title_full_unstemmed |
Development of Loop-Mediated Isothermal Amplification–Based Lateral Flow Device Method for the Detection of Malaria |
| title_sort |
development of loop-mediated isothermal amplification–based lateral flow device method for the detection of malaria |
| publisher |
American Society of Tropical Medicine and Hygiene |
| publishDate |
2018 |
| url |
http://eprints.um.edu.my/21352/ https://doi.org/10.4269/ajtmh.18-0177 |
| _version_ |
1643691540127678464 |
| score |
13.244367 |