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Testosterone Down-regulates Expression of αVβ3-integrin, E-cadherin and Mucin-1 during Uterine Receptivity Period in Rats

Adequate development of uterine receptivity is crucial for establishment of pregnancy. Expression of uterine receptivity molecules i.e. αvβ3 integrin, E-cadherin and mucin-1 could be affected by testosterone. The objective of this study was to investigate effect of testosterone on expression of thes...

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Main Authors: Mohd Mokhtar, Helmy, Giribabu, Nelli, Salleh, Naguib
Format: Article
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/21225/
https://doi.org/10.17576/jsm-2018-4710-28
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spelling my.um.eprints.212252019-05-14T08:14:04Z http://eprints.um.edu.my/21225/ Testosterone Down-regulates Expression of αVβ3-integrin, E-cadherin and Mucin-1 during Uterine Receptivity Period in Rats Mohd Mokhtar, Helmy Giribabu, Nelli Salleh, Naguib R Medicine Adequate development of uterine receptivity is crucial for establishment of pregnancy. Expression of uterine receptivity molecules i.e. αvβ3 integrin, E-cadherin and mucin-1 could be affected by testosterone. The objective of this study was to investigate effect of testosterone on expression of these molecules during early pregnancy. 30 ovariectomised female Sprague-Dawley rats were divided into 5 groups that consisted of vehicle control, rats received eight days sex-steroid replacement regime (intended to mimic the hormonal changes in early pregnancy) and three groups of rats given testosterone (1 mg/kg/day) subcutaneously with or without flutamide or finasteride between day 6 and 8 representing the period of uterine receptivity. At the end of the treatment, rats were sacrificed and uteri were removed. Expression and distribution of αvβ3 integrin, E-cadherin and mucin-1 were examined by immunoflourescence and levels of messenger RNA (mRNAs) were evaluated by real-time PCR. Expression of αvβ3 integrin, E-cadherin and mucin-1 in the uteri of rats receiving sex-steroid replacement regime increased significantly as compared to control (p<0.05). In these rats, concomitant administration of testosterone between day 6 and 8 resulted in expression of αvβ3 integrin, E-cadherin and mucin-1 to decrease significantly (p<0.05) as compared to rats receiving sex-steroid replacement regime without testosterone treatment. Moreover, the testosterone effects were not antagonized by either flutamide or finasteride. As a result, reduced expression of uterine receptivity molecules by testosterone might interfere with early pregnancy establishment, therefore could adversely affect the female fertility. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed Mohd Mokhtar, Helmy and Giribabu, Nelli and Salleh, Naguib (2018) Testosterone Down-regulates Expression of αVβ3-integrin, E-cadherin and Mucin-1 during Uterine Receptivity Period in Rats. Sains Malaysiana, 47 (10). pp. 2509-2517. ISSN 0126-6039 https://doi.org/10.17576/jsm-2018-4710-28 doi:10.17576/jsm-2018-4710-28
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Mohd Mokhtar, Helmy
Giribabu, Nelli
Salleh, Naguib
Testosterone Down-regulates Expression of αVβ3-integrin, E-cadherin and Mucin-1 during Uterine Receptivity Period in Rats
description Adequate development of uterine receptivity is crucial for establishment of pregnancy. Expression of uterine receptivity molecules i.e. αvβ3 integrin, E-cadherin and mucin-1 could be affected by testosterone. The objective of this study was to investigate effect of testosterone on expression of these molecules during early pregnancy. 30 ovariectomised female Sprague-Dawley rats were divided into 5 groups that consisted of vehicle control, rats received eight days sex-steroid replacement regime (intended to mimic the hormonal changes in early pregnancy) and three groups of rats given testosterone (1 mg/kg/day) subcutaneously with or without flutamide or finasteride between day 6 and 8 representing the period of uterine receptivity. At the end of the treatment, rats were sacrificed and uteri were removed. Expression and distribution of αvβ3 integrin, E-cadherin and mucin-1 were examined by immunoflourescence and levels of messenger RNA (mRNAs) were evaluated by real-time PCR. Expression of αvβ3 integrin, E-cadherin and mucin-1 in the uteri of rats receiving sex-steroid replacement regime increased significantly as compared to control (p<0.05). In these rats, concomitant administration of testosterone between day 6 and 8 resulted in expression of αvβ3 integrin, E-cadherin and mucin-1 to decrease significantly (p<0.05) as compared to rats receiving sex-steroid replacement regime without testosterone treatment. Moreover, the testosterone effects were not antagonized by either flutamide or finasteride. As a result, reduced expression of uterine receptivity molecules by testosterone might interfere with early pregnancy establishment, therefore could adversely affect the female fertility.
format Article
author Mohd Mokhtar, Helmy
Giribabu, Nelli
Salleh, Naguib
author_facet Mohd Mokhtar, Helmy
Giribabu, Nelli
Salleh, Naguib
author_sort Mohd Mokhtar, Helmy
title Testosterone Down-regulates Expression of αVβ3-integrin, E-cadherin and Mucin-1 during Uterine Receptivity Period in Rats
title_short Testosterone Down-regulates Expression of αVβ3-integrin, E-cadherin and Mucin-1 during Uterine Receptivity Period in Rats
title_full Testosterone Down-regulates Expression of αVβ3-integrin, E-cadherin and Mucin-1 during Uterine Receptivity Period in Rats
title_fullStr Testosterone Down-regulates Expression of αVβ3-integrin, E-cadherin and Mucin-1 during Uterine Receptivity Period in Rats
title_full_unstemmed Testosterone Down-regulates Expression of αVβ3-integrin, E-cadherin and Mucin-1 during Uterine Receptivity Period in Rats
title_sort testosterone down-regulates expression of αvβ3-integrin, e-cadherin and mucin-1 during uterine receptivity period in rats
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://eprints.um.edu.my/21225/
https://doi.org/10.17576/jsm-2018-4710-28
_version_ 1643691502736506880
score 13.211869

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