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Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king

This study aimed to isolate compounds from Polyalthia lateriflora (Bl.) King stem bark, and to test the crude extracts and the isolated compounds for their antioxidant and cytotoxic activities. Three different solvents were used to prepare the crude extracts; hexane, dichloromethane (DCM) and methan...

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Main Authors:	Obaid, Ahmed Kareem, Abdul Azziz, Saripah Salbiah Syed, Bakri, Yuhanis Mhd, Nafiah, Mohd Azlan, Awang, Khalijah, Aowda, Saadon Abdulla, Hassan, Norazian Mohd, Hashim, Yumi Zuhanis Has Yun, Abdullah, Fauziah Hanum
Format:	Article
Published:	Pharmainfo Publications 2018
Subjects:	Q Science (General) QD Chemistry
Online Access:	http://eprints.um.edu.my/21090/ https://www.jpsr.pharmainfo.in/Documents/Volumes/vol10Issue11/jpsr10111807.pdf
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spelling	my.um.eprints.210902019-04-26T08:44:14Z http://eprints.um.edu.my/21090/ Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king Obaid, Ahmed Kareem Abdul Azziz, Saripah Salbiah Syed Bakri, Yuhanis Mhd Nafiah, Mohd Azlan Awang, Khalijah Aowda, Saadon Abdulla Hassan, Norazian Mohd Hashim, Yumi Zuhanis Has Yun Abdullah, Fauziah Hanum Q Science (General) QD Chemistry This study aimed to isolate compounds from Polyalthia lateriflora (Bl.) King stem bark, and to test the crude extracts and the isolated compounds for their antioxidant and cytotoxic activities. Three different solvents were used to prepare the crude extracts; hexane, dichloromethane (DCM) and methanol. Phytochemical investigation of the crude extracts has led to the isolation of four oxoaporphine alkaloids (O-methylmoschatoline, atherospermidine, lysicamine, and liriodenine) and two steroids (stigmasterol and lupeol). The chemical structure of the compounds was elucidated using1 H-NMR,13 C-NMR, 2D-NMR, and mass spectral analysis. All the crude extracts and isolated compounds were tested for their in vitro DPPH radicals scavenging activity and cytotoxicity against MCF-7 breast cancer cells. Methanol extract showed the strongest antioxidant activity with percentage inhibition at 500µg/mL of 89.52±1.81 (IC50, 128.22± 6.73 µg/ml) in comparison with DCM (32.45±8.56), atherospermidine (20.17±9.74) and liriodenine (10.73±0.45), while other extracts were inactive. All the isolated compounds showed cytotoxic against MCF-7 cells, at IC50 values in the range of 42-89 µg/ml. Based on thorough literature review, all compounds identified in this study were isolated for the first time from this plant. In particular, lupeol is reported for the first time in the genus Polyalthia and possessed the most potent cytotoxic effect compared to other isolated compounds. Pharmainfo Publications 2018 Article PeerReviewed Obaid, Ahmed Kareem and Abdul Azziz, Saripah Salbiah Syed and Bakri, Yuhanis Mhd and Nafiah, Mohd Azlan and Awang, Khalijah and Aowda, Saadon Abdulla and Hassan, Norazian Mohd and Hashim, Yumi Zuhanis Has Yun and Abdullah, Fauziah Hanum (2018) Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king. Journal of Pharmaceutical Sciences and Research, 10 (11). pp. 2718-2721. ISSN 0975-1459 https://www.jpsr.pharmainfo.in/Documents/Volumes/vol10Issue11/jpsr10111807.pdf
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QD Chemistry
spellingShingle	Q Science (General) QD Chemistry Obaid, Ahmed Kareem Abdul Azziz, Saripah Salbiah Syed Bakri, Yuhanis Mhd Nafiah, Mohd Azlan Awang, Khalijah Aowda, Saadon Abdulla Hassan, Norazian Mohd Hashim, Yumi Zuhanis Has Yun Abdullah, Fauziah Hanum Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king
description	This study aimed to isolate compounds from Polyalthia lateriflora (Bl.) King stem bark, and to test the crude extracts and the isolated compounds for their antioxidant and cytotoxic activities. Three different solvents were used to prepare the crude extracts; hexane, dichloromethane (DCM) and methanol. Phytochemical investigation of the crude extracts has led to the isolation of four oxoaporphine alkaloids (O-methylmoschatoline, atherospermidine, lysicamine, and liriodenine) and two steroids (stigmasterol and lupeol). The chemical structure of the compounds was elucidated using1 H-NMR,13 C-NMR, 2D-NMR, and mass spectral analysis. All the crude extracts and isolated compounds were tested for their in vitro DPPH radicals scavenging activity and cytotoxicity against MCF-7 breast cancer cells. Methanol extract showed the strongest antioxidant activity with percentage inhibition at 500µg/mL of 89.52±1.81 (IC50, 128.22± 6.73 µg/ml) in comparison with DCM (32.45±8.56), atherospermidine (20.17±9.74) and liriodenine (10.73±0.45), while other extracts were inactive. All the isolated compounds showed cytotoxic against MCF-7 cells, at IC50 values in the range of 42-89 µg/ml. Based on thorough literature review, all compounds identified in this study were isolated for the first time from this plant. In particular, lupeol is reported for the first time in the genus Polyalthia and possessed the most potent cytotoxic effect compared to other isolated compounds.
format	Article
author	Obaid, Ahmed Kareem Abdul Azziz, Saripah Salbiah Syed Bakri, Yuhanis Mhd Nafiah, Mohd Azlan Awang, Khalijah Aowda, Saadon Abdulla Hassan, Norazian Mohd Hashim, Yumi Zuhanis Has Yun Abdullah, Fauziah Hanum
author_facet	Obaid, Ahmed Kareem Abdul Azziz, Saripah Salbiah Syed Bakri, Yuhanis Mhd Nafiah, Mohd Azlan Awang, Khalijah Aowda, Saadon Abdulla Hassan, Norazian Mohd Hashim, Yumi Zuhanis Has Yun Abdullah, Fauziah Hanum
author_sort	Obaid, Ahmed Kareem
title	Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king
title_short	Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king
title_full	Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king
title_fullStr	Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king
title_full_unstemmed	Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king
title_sort	antioxidative and cytotoxic activities of crude and isolated compounds of p. lateriflora (bl.) king
publisher	Pharmainfo Publications
publishDate	2018
url	http://eprints.um.edu.my/21090/ https://www.jpsr.pharmainfo.in/Documents/Volumes/vol10Issue11/jpsr10111807.pdf
_version_	1643691463304806400
score	13.211869

Antioxidative and cytotoxic activities of crude and isolated compounds of p. Lateriflora (bl.) king

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