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Accumulation of terpenoid indole alkaloids in jasmonic acid elicited catharanthus roseus plants before and during flowering

Jasmonates analogues including jasmonic acid (JA) and methyl-jasmonate (MeJA) are plant-signaling molecules involved in defense against insects and pathogens. In Catharanthus roseus, jasmonates play a key role in regulating the biosynthesis of pharmaceutically important terpenoid indole alkaloids (T...

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Main Authors: Pan, Qi Fang, Saiman, Mohd Zuwairi, Verpoorte, Robert, Tang, Kexuan
Format: Article
Language:English
Published: Pakistan Botanical Society 2018
Subjects:
Q Science (General)
QH Natural history
Online Access:http://eprints.um.edu.my/21047/1/MohdZuwairiSaiman2018.pdf
http://eprints.um.edu.my/21047/
http://www.pakbs.org/
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spelling my.um.eprints.210472019-04-24T03:48:49Z http://eprints.um.edu.my/21047/ Accumulation of terpenoid indole alkaloids in jasmonic acid elicited catharanthus roseus plants before and during flowering Pan, Qi Fang Saiman, Mohd Zuwairi Verpoorte, Robert Tang, Kexuan Q Science (General) QH Natural history Jasmonates analogues including jasmonic acid (JA) and methyl-jasmonate (MeJA) are plant-signaling molecules involved in defense against insects and pathogens. In Catharanthus roseus, jasmonates play a key role in regulating the biosynthesis of pharmaceutically important terpenoid indole alkaloids (TIAs). In the present study, C. roseus plants were elicited with JA before and during flowering to investigate the induction of TIA biosynthesis in different organs during the two developmental stages concerning via HPLC and qRT-PCR methods. The results showed that JA stimulates the TIA accumulation before flowering but had less effect during flowering. TIA accumulations in different organs (flower, leaf, and root) also showed a different response to JA elicitation. Moreover, transcriptional analysis showed that JA elicitation had a greater effect on the expression levels of key TIA biosynthetic genes (such as STR, SGD, DAT and PRX1) in C. roseus before flowering than during flowering. In C. roseus control (non-treated) plants the level of jasmonic acid was higher before flowering than during flowering, and in JA-treated plants JA was accumulated more before flowering than during flowering. The study provided an insight into the effect of flowering on JA-induced TIA biosynthesis in C. roseus plants. Pakistan Botanical Society 2018 Article PeerReviewed text en http://eprints.um.edu.my/21047/1/MohdZuwairiSaiman2018.pdf Pan, Qi Fang and Saiman, Mohd Zuwairi and Verpoorte, Robert and Tang, Kexuan (2018) Accumulation of terpenoid indole alkaloids in jasmonic acid elicited catharanthus roseus plants before and during flowering. Pakistan Journal of Botany, 50 (3). pp. 1077-1083. ISSN 0556-3321 http://www.pakbs.org/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Pan, Qi Fang
Saiman, Mohd Zuwairi
Verpoorte, Robert
Tang, Kexuan
Accumulation of terpenoid indole alkaloids in jasmonic acid elicited catharanthus roseus plants before and during flowering
description Jasmonates analogues including jasmonic acid (JA) and methyl-jasmonate (MeJA) are plant-signaling molecules involved in defense against insects and pathogens. In Catharanthus roseus, jasmonates play a key role in regulating the biosynthesis of pharmaceutically important terpenoid indole alkaloids (TIAs). In the present study, C. roseus plants were elicited with JA before and during flowering to investigate the induction of TIA biosynthesis in different organs during the two developmental stages concerning via HPLC and qRT-PCR methods. The results showed that JA stimulates the TIA accumulation before flowering but had less effect during flowering. TIA accumulations in different organs (flower, leaf, and root) also showed a different response to JA elicitation. Moreover, transcriptional analysis showed that JA elicitation had a greater effect on the expression levels of key TIA biosynthetic genes (such as STR, SGD, DAT and PRX1) in C. roseus before flowering than during flowering. In C. roseus control (non-treated) plants the level of jasmonic acid was higher before flowering than during flowering, and in JA-treated plants JA was accumulated more before flowering than during flowering. The study provided an insight into the effect of flowering on JA-induced TIA biosynthesis in C. roseus plants.
format Article
author Pan, Qi Fang
Saiman, Mohd Zuwairi
Verpoorte, Robert
Tang, Kexuan
author_facet Pan, Qi Fang
Saiman, Mohd Zuwairi
Verpoorte, Robert
Tang, Kexuan
author_sort Pan, Qi Fang
title Accumulation of terpenoid indole alkaloids in jasmonic acid elicited catharanthus roseus plants before and during flowering
title_short Accumulation of terpenoid indole alkaloids in jasmonic acid elicited catharanthus roseus plants before and during flowering
title_full Accumulation of terpenoid indole alkaloids in jasmonic acid elicited catharanthus roseus plants before and during flowering
title_fullStr Accumulation of terpenoid indole alkaloids in jasmonic acid elicited catharanthus roseus plants before and during flowering
title_full_unstemmed Accumulation of terpenoid indole alkaloids in jasmonic acid elicited catharanthus roseus plants before and during flowering
title_sort accumulation of terpenoid indole alkaloids in jasmonic acid elicited catharanthus roseus plants before and during flowering
publisher Pakistan Botanical Society
publishDate 2018
url http://eprints.um.edu.my/21047/1/MohdZuwairiSaiman2018.pdf
http://eprints.um.edu.my/21047/
http://www.pakbs.org/
_version_ 1643691451215773696
score 13.211869

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