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A microfluidic Lab-on-a-Disc (LOD) for antioxidant activities of plant extracts

Antioxidants are an important substance that can fight the deterioration of free radicals and can easily oxidize when exposed to light. There are many methods to measure the antioxidant activity in a biological sample, for example 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity test, which...

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Main Authors: Rahman, Nurhaslina Abd, Ibrahim, Fatimah, Aeinehvand, Mohammad Mahdi, Yusof, Rohana, Madou, Marc J.
Format: Article
Published: MDPI 2018
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/20963/
https://doi.org/10.3390/mi9040140
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spelling my.um.eprints.209632019-04-17T07:54:53Z http://eprints.um.edu.my/20963/ A microfluidic Lab-on-a-Disc (LOD) for antioxidant activities of plant extracts Rahman, Nurhaslina Abd Ibrahim, Fatimah Aeinehvand, Mohammad Mahdi Yusof, Rohana Madou, Marc J. R Medicine Antioxidants are an important substance that can fight the deterioration of free radicals and can easily oxidize when exposed to light. There are many methods to measure the antioxidant activity in a biological sample, for example 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity test, which is one of the simplest methods used. Despite its simplicity, the organic solvent that has been used to dilute DPPH is easily evaporated and degraded with respect to light exposure and time. Thus, it needs to be used at the earliest convenient time prior to the experiment. To overcome this issue, a rapid and close system for antioxidant activity is required. In this paper, we introduced the Lab-on-a-Disc (LoD) method that integrates the DPPH antioxidant activity test on a microfluidic compact disc (CD).We used ascorbic acid, quercetin, Areca catechu, Polygonum minus, and Syzygium polyanthum plant extracts to compare the results of our proposed LoD method with the conventional method. Contrasted to the arduous laborious conventional method, our proposed method offer rapid analysis and simple determination of antioxidant. This proposed LoD method for antioxidant activity in plants would be a platform for the further development of antioxidant assay. MDPI 2018 Article PeerReviewed Rahman, Nurhaslina Abd and Ibrahim, Fatimah and Aeinehvand, Mohammad Mahdi and Yusof, Rohana and Madou, Marc J. (2018) A microfluidic Lab-on-a-Disc (LOD) for antioxidant activities of plant extracts. Micromachines, 9 (4). p. 140. ISSN 2072-666X https://doi.org/10.3390/mi9040140
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Rahman, Nurhaslina Abd
Ibrahim, Fatimah
Aeinehvand, Mohammad Mahdi
Yusof, Rohana
Madou, Marc J.
A microfluidic Lab-on-a-Disc (LOD) for antioxidant activities of plant extracts
description Antioxidants are an important substance that can fight the deterioration of free radicals and can easily oxidize when exposed to light. There are many methods to measure the antioxidant activity in a biological sample, for example 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity test, which is one of the simplest methods used. Despite its simplicity, the organic solvent that has been used to dilute DPPH is easily evaporated and degraded with respect to light exposure and time. Thus, it needs to be used at the earliest convenient time prior to the experiment. To overcome this issue, a rapid and close system for antioxidant activity is required. In this paper, we introduced the Lab-on-a-Disc (LoD) method that integrates the DPPH antioxidant activity test on a microfluidic compact disc (CD).We used ascorbic acid, quercetin, Areca catechu, Polygonum minus, and Syzygium polyanthum plant extracts to compare the results of our proposed LoD method with the conventional method. Contrasted to the arduous laborious conventional method, our proposed method offer rapid analysis and simple determination of antioxidant. This proposed LoD method for antioxidant activity in plants would be a platform for the further development of antioxidant assay.
format Article
author Rahman, Nurhaslina Abd
Ibrahim, Fatimah
Aeinehvand, Mohammad Mahdi
Yusof, Rohana
Madou, Marc J.
author_facet Rahman, Nurhaslina Abd
Ibrahim, Fatimah
Aeinehvand, Mohammad Mahdi
Yusof, Rohana
Madou, Marc J.
author_sort Rahman, Nurhaslina Abd
title A microfluidic Lab-on-a-Disc (LOD) for antioxidant activities of plant extracts
title_short A microfluidic Lab-on-a-Disc (LOD) for antioxidant activities of plant extracts
title_full A microfluidic Lab-on-a-Disc (LOD) for antioxidant activities of plant extracts
title_fullStr A microfluidic Lab-on-a-Disc (LOD) for antioxidant activities of plant extracts
title_full_unstemmed A microfluidic Lab-on-a-Disc (LOD) for antioxidant activities of plant extracts
title_sort microfluidic lab-on-a-disc (lod) for antioxidant activities of plant extracts
publisher MDPI
publishDate 2018
url http://eprints.um.edu.my/20963/
https://doi.org/10.3390/mi9040140
_version_ 1643691428734304256
score 13.211869

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