Cover Image

Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide

This experiment evaluated Panduratin A (PA), a chalcone isolated from Boesenbergia rotunda rhizomes, for its hepatoprotectivity. Rats were subjected to liver damage induced by intra-peritoneal injection of thioacetamide (TAA). PA was tested first for its acute toxicity and then administered by oral...

Full description

Saved in:
Bibliographic Details
Main Authors: Salama, Suzy Munir, Ibrahim, Ibrahim Abdel Aziz, Shahzad, Naiyer, Al-Ghamdi, Saeed, Ayoub, Nahla, AlRashdi, Ahmed Salim, Abdulla, Mahmood Ameen, Salehen, Nur'ain, Bilgen, Mehmet
Format: Article
Published: Wiley 2018
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/20805/
https://doi.org/10.1111/apm.12878
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.20805
record_format eprints
spelling my.um.eprints.208052019-04-03T09:06:16Z http://eprints.um.edu.my/20805/ Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide Salama, Suzy Munir Ibrahim, Ibrahim Abdel Aziz Shahzad, Naiyer Al-Ghamdi, Saeed Ayoub, Nahla AlRashdi, Ahmed Salim Abdulla, Mahmood Ameen Salehen, Nur'ain Bilgen, Mehmet R Medicine This experiment evaluated Panduratin A (PA), a chalcone isolated from Boesenbergia rotunda rhizomes, for its hepatoprotectivity. Rats were subjected to liver damage induced by intra-peritoneal injection of thioacetamide (TAA). PA was tested first for its acute toxicity and then administered by oral gavage at doses 5, 10, and 50 mg/kg to rats. At the end of the 8th week, livers from all rats were excised and evaluated ex vivo. Measurements included alkaline phosphatase (AP), alanine transaminase (ALT), aspartate transaminase (AST) and gamma-glutamyl transferase (GGT), serum platelet-derived growth factor (PDGF) and transforming growth factor (TGF-β1), and hepatic metalloproteinase enzyme (MMP-2) and its inhibitor extracellular matrix protein (TIMP-1). Oxidative stress was measured by liver malondialdehyde (MDA) and nitrotyrosine levels, urinary 8-hydroxy 2- deoxyguanosine (8-OH-dG), and hepatic antioxidant enzyme activities. The immunohistochemistry of TGF-β1 was additionally performed. PA revealed safe dose of 250 mg/kg on experimental rats and positive effect on the liver. The results suggested reduced hepatic stellate cells (HSCs) activity as verified from the attenuation of serum PDGF and TGF-β1, hepatic MMP-2 and TIMP-1, and oxidative stress. The extensive data altogether conclude that PA treatment could protect the liver from the progression of cirrhosis through a possible mechanism inhibiting HSCs activity. Wiley 2018 Article PeerReviewed Salama, Suzy Munir and Ibrahim, Ibrahim Abdel Aziz and Shahzad, Naiyer and Al-Ghamdi, Saeed and Ayoub, Nahla and AlRashdi, Ahmed Salim and Abdulla, Mahmood Ameen and Salehen, Nur'ain and Bilgen, Mehmet (2018) Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide. APMIS, 126 (9). pp. 710-721. ISSN 0903-4641 https://doi.org/10.1111/apm.12878 doi:10.1111/apm.12878
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Salama, Suzy Munir
Ibrahim, Ibrahim Abdel Aziz
Shahzad, Naiyer
Al-Ghamdi, Saeed
Ayoub, Nahla
AlRashdi, Ahmed Salim
Abdulla, Mahmood Ameen
Salehen, Nur'ain
Bilgen, Mehmet
Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide
description This experiment evaluated Panduratin A (PA), a chalcone isolated from Boesenbergia rotunda rhizomes, for its hepatoprotectivity. Rats were subjected to liver damage induced by intra-peritoneal injection of thioacetamide (TAA). PA was tested first for its acute toxicity and then administered by oral gavage at doses 5, 10, and 50 mg/kg to rats. At the end of the 8th week, livers from all rats were excised and evaluated ex vivo. Measurements included alkaline phosphatase (AP), alanine transaminase (ALT), aspartate transaminase (AST) and gamma-glutamyl transferase (GGT), serum platelet-derived growth factor (PDGF) and transforming growth factor (TGF-β1), and hepatic metalloproteinase enzyme (MMP-2) and its inhibitor extracellular matrix protein (TIMP-1). Oxidative stress was measured by liver malondialdehyde (MDA) and nitrotyrosine levels, urinary 8-hydroxy 2- deoxyguanosine (8-OH-dG), and hepatic antioxidant enzyme activities. The immunohistochemistry of TGF-β1 was additionally performed. PA revealed safe dose of 250 mg/kg on experimental rats and positive effect on the liver. The results suggested reduced hepatic stellate cells (HSCs) activity as verified from the attenuation of serum PDGF and TGF-β1, hepatic MMP-2 and TIMP-1, and oxidative stress. The extensive data altogether conclude that PA treatment could protect the liver from the progression of cirrhosis through a possible mechanism inhibiting HSCs activity.
format Article
author Salama, Suzy Munir
Ibrahim, Ibrahim Abdel Aziz
Shahzad, Naiyer
Al-Ghamdi, Saeed
Ayoub, Nahla
AlRashdi, Ahmed Salim
Abdulla, Mahmood Ameen
Salehen, Nur'ain
Bilgen, Mehmet
author_facet Salama, Suzy Munir
Ibrahim, Ibrahim Abdel Aziz
Shahzad, Naiyer
Al-Ghamdi, Saeed
Ayoub, Nahla
AlRashdi, Ahmed Salim
Abdulla, Mahmood Ameen
Salehen, Nur'ain
Bilgen, Mehmet
author_sort Salama, Suzy Munir
title Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide
title_short Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide
title_full Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide
title_fullStr Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide
title_full_unstemmed Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide
title_sort hepatoprotectivity of panduratin a against liver damage: in vivo demonstration with a rat model of cirrhosis induced by thioacetamide
publisher Wiley
publishDate 2018
url http://eprints.um.edu.my/20805/
https://doi.org/10.1111/apm.12878
_version_ 1643691384892293120
score 13.211869

Similar Items