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Integration process of fermentation and liquid biphasic flotation for lipase separation from Burkholderia cepacia

Liquid Biphasic Flotation (LBF) is an advanced recovery method that has been effectively applied for biomolecules extraction. The objective of this investigation is to incorporate the fermentation and extraction process of lipase from Burkholderia cepacia using flotation system. Initial study was co...

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Main Authors: Sankaran, Revathy, Show, Pau Loke, Lee, Sze Ying, Yap, Yee Jiun, Ling, Tau Chuan
Format: Article
Published: Elsevier 2018
Subjects:
Q Science (General)
QH Natural history
Online Access:http://eprints.um.edu.my/20779/
https://doi.org/10.1016/j.biortech.2017.11.050
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spelling my.um.eprints.207792019-03-22T02:02:59Z http://eprints.um.edu.my/20779/ Integration process of fermentation and liquid biphasic flotation for lipase separation from Burkholderia cepacia Sankaran, Revathy Show, Pau Loke Lee, Sze Ying Yap, Yee Jiun Ling, Tau Chuan Q Science (General) QH Natural history Liquid Biphasic Flotation (LBF) is an advanced recovery method that has been effectively applied for biomolecules extraction. The objective of this investigation is to incorporate the fermentation and extraction process of lipase from Burkholderia cepacia using flotation system. Initial study was conducted to compare the performance of bacteria growth and lipase production using flotation and shaker system. From the results obtained, bacteria shows quicker growth and high lipase yield via flotation system. Integration process for lipase separation was investigated and the result showed high efficiency reaching 92.29% and yield of 95.73%. Upscaling of the flotation system exhibited consistent result with the lab-scale which are 89.53% efficiency and 93.82% yield. The combination of upstream and downstream processes in a single system enables the acceleration of product formation, improves the product yield and facilitates downstream processing. This integration system demonstrated its potential for biomolecules fermentation and separation that possibly open new opportunities for industrial production. Elsevier 2018 Article PeerReviewed Sankaran, Revathy and Show, Pau Loke and Lee, Sze Ying and Yap, Yee Jiun and Ling, Tau Chuan (2018) Integration process of fermentation and liquid biphasic flotation for lipase separation from Burkholderia cepacia. Bioresource Technology, 250. pp. 306-316. ISSN 0960-8524 https://doi.org/10.1016/j.biortech.2017.11.050 doi:10.1016/j.biortech.2017.11.050
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Sankaran, Revathy
Show, Pau Loke
Lee, Sze Ying
Yap, Yee Jiun
Ling, Tau Chuan
Integration process of fermentation and liquid biphasic flotation for lipase separation from Burkholderia cepacia
description Liquid Biphasic Flotation (LBF) is an advanced recovery method that has been effectively applied for biomolecules extraction. The objective of this investigation is to incorporate the fermentation and extraction process of lipase from Burkholderia cepacia using flotation system. Initial study was conducted to compare the performance of bacteria growth and lipase production using flotation and shaker system. From the results obtained, bacteria shows quicker growth and high lipase yield via flotation system. Integration process for lipase separation was investigated and the result showed high efficiency reaching 92.29% and yield of 95.73%. Upscaling of the flotation system exhibited consistent result with the lab-scale which are 89.53% efficiency and 93.82% yield. The combination of upstream and downstream processes in a single system enables the acceleration of product formation, improves the product yield and facilitates downstream processing. This integration system demonstrated its potential for biomolecules fermentation and separation that possibly open new opportunities for industrial production.
format Article
author Sankaran, Revathy
Show, Pau Loke
Lee, Sze Ying
Yap, Yee Jiun
Ling, Tau Chuan
author_facet Sankaran, Revathy
Show, Pau Loke
Lee, Sze Ying
Yap, Yee Jiun
Ling, Tau Chuan
author_sort Sankaran, Revathy
title Integration process of fermentation and liquid biphasic flotation for lipase separation from Burkholderia cepacia
title_short Integration process of fermentation and liquid biphasic flotation for lipase separation from Burkholderia cepacia
title_full Integration process of fermentation and liquid biphasic flotation for lipase separation from Burkholderia cepacia
title_fullStr Integration process of fermentation and liquid biphasic flotation for lipase separation from Burkholderia cepacia
title_full_unstemmed Integration process of fermentation and liquid biphasic flotation for lipase separation from Burkholderia cepacia
title_sort integration process of fermentation and liquid biphasic flotation for lipase separation from burkholderia cepacia
publisher Elsevier
publishDate 2018
url http://eprints.um.edu.my/20779/
https://doi.org/10.1016/j.biortech.2017.11.050
_version_ 1643691377628807168
score 13.211869

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