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Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains

Listeria monocytogenes is a major foodborne pathogen causing increased morbidity worldwide. It forms resistant biofilm structures in food processing facilities after sanitization, consequently creating a public health concern. Many studies on the metabolism and transmission of L. monocytogenes has p...

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Main Authors: Sharar, Nowshin Shyara, Chai, Lay Ching, Thong, Kwai Lin
Format: Article
Published: Springer International Publishing 2018
Subjects:
Q Science (General)
QH Natural history
Online Access:http://eprints.um.edu.my/20707/
https://doi.org/10.1007/s00003-018-1169-6
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spelling my.um.eprints.207072019-03-18T03:15:41Z http://eprints.um.edu.my/20707/ Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains Sharar, Nowshin Shyara Chai, Lay Ching Thong, Kwai Lin Q Science (General) QH Natural history Listeria monocytogenes is a major foodborne pathogen causing increased morbidity worldwide. It forms resistant biofilm structures in food processing facilities after sanitization, consequently creating a public health concern. Many studies on the metabolism and transmission of L. monocytogenes has provided insights into its intracellular infection process, however there is limited understanding on the substrate utilization of the bacteria. Therefore, the main objective of this study was to investigate the carbon and nitrogen substrate catabolism and the biofilm forming potential of 3 Malaysian L. monocytogenes strains (LM41, LM92 and LM115) previously isolated from ready-to-eat foods. Biolog Phenotype Microarray (PM) system was used to study the catabolic activity of the foodborne strains in 190 carbon and 380 nitrogen sources. PM analysis showed that the carbon and nitrogen catabolic activity of L. monocytogenes strains were considerably limited and these strains utilised Tween 40 and Tween 80, which are commonly used for the sanitation in food and meat processing industries. Furthermore, all 3 strains showed strong biofilm forming potential in nutrient-rich and nutrient-limited media, irrespective of the serogroups. The data generated could be utilised to develop alternative measure to inhibit biofilm formation in L. monocytogenes in the food processing environment. Springer International Publishing 2018 Article PeerReviewed Sharar, Nowshin Shyara and Chai, Lay Ching and Thong, Kwai Lin (2018) Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains. Journal of Consumer Protection and Food Safety, 13 (3). pp. 289-298. ISSN 1661-5751 https://doi.org/10.1007/s00003-018-1169-6 doi:10.1007/s00003-018-1169-6
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Sharar, Nowshin Shyara
Chai, Lay Ching
Thong, Kwai Lin
Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains
description Listeria monocytogenes is a major foodborne pathogen causing increased morbidity worldwide. It forms resistant biofilm structures in food processing facilities after sanitization, consequently creating a public health concern. Many studies on the metabolism and transmission of L. monocytogenes has provided insights into its intracellular infection process, however there is limited understanding on the substrate utilization of the bacteria. Therefore, the main objective of this study was to investigate the carbon and nitrogen substrate catabolism and the biofilm forming potential of 3 Malaysian L. monocytogenes strains (LM41, LM92 and LM115) previously isolated from ready-to-eat foods. Biolog Phenotype Microarray (PM) system was used to study the catabolic activity of the foodborne strains in 190 carbon and 380 nitrogen sources. PM analysis showed that the carbon and nitrogen catabolic activity of L. monocytogenes strains were considerably limited and these strains utilised Tween 40 and Tween 80, which are commonly used for the sanitation in food and meat processing industries. Furthermore, all 3 strains showed strong biofilm forming potential in nutrient-rich and nutrient-limited media, irrespective of the serogroups. The data generated could be utilised to develop alternative measure to inhibit biofilm formation in L. monocytogenes in the food processing environment.
format Article
author Sharar, Nowshin Shyara
Chai, Lay Ching
Thong, Kwai Lin
author_facet Sharar, Nowshin Shyara
Chai, Lay Ching
Thong, Kwai Lin
author_sort Sharar, Nowshin Shyara
title Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains
title_short Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains
title_full Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains
title_fullStr Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains
title_full_unstemmed Catabolic activity and biofilm formation of foodborne Listeria monocytogenes strains
title_sort catabolic activity and biofilm formation of foodborne listeria monocytogenes strains
publisher Springer International Publishing
publishDate 2018
url http://eprints.um.edu.my/20707/
https://doi.org/10.1007/s00003-018-1169-6
_version_ 1643691356241002496
score 13.211869

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