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Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana

The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems a...

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Main Authors: Gan, Khai Swan, Tan, Chon Seng, Othman, Rofina Yasmin, Harikrishna, Jennifer Ann
Format: Article
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Subjects:
Q Science (General)
QH Natural history
Online Access:http://eprints.um.edu.my/20589/
https://doi.org/10.17576/jsm-2018-4703-09
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spelling my.um.eprints.205892019-03-04T08:17:05Z http://eprints.um.edu.my/20589/ Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana Gan, Khai Swan Tan, Chon Seng Othman, Rofina Yasmin Harikrishna, Jennifer Ann Q Science (General) QH Natural history The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems are gaining recognition as a viable alternative for the production of vaccine antigens. In this study, we expressed the Nipah virus G protein heterologously in Nicotiana benthamiana using an agroinfiltration approach. The highest expression of recombinant G protein in N. benthamiana at RNA and protein levels was detected on day 9 post-infiltration. Western blot analysis demonstrated that the purified G protein reacted specifically with rabbit anti-Nipah Virus serum, indicating its potential for vaccine use. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed Gan, Khai Swan and Tan, Chon Seng and Othman, Rofina Yasmin and Harikrishna, Jennifer Ann (2018) Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana. Sains Malaysiana, 47 (3). pp. 499-509. ISSN 0126-6039 https://doi.org/10.17576/jsm-2018-4703-09 doi:10.17576/jsm-2018-4703-09
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Gan, Khai Swan
Tan, Chon Seng
Othman, Rofina Yasmin
Harikrishna, Jennifer Ann
Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana
description The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems are gaining recognition as a viable alternative for the production of vaccine antigens. In this study, we expressed the Nipah virus G protein heterologously in Nicotiana benthamiana using an agroinfiltration approach. The highest expression of recombinant G protein in N. benthamiana at RNA and protein levels was detected on day 9 post-infiltration. Western blot analysis demonstrated that the purified G protein reacted specifically with rabbit anti-Nipah Virus serum, indicating its potential for vaccine use.
format Article
author Gan, Khai Swan
Tan, Chon Seng
Othman, Rofina Yasmin
Harikrishna, Jennifer Ann
author_facet Gan, Khai Swan
Tan, Chon Seng
Othman, Rofina Yasmin
Harikrishna, Jennifer Ann
author_sort Gan, Khai Swan
title Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana
title_short Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana
title_full Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana
title_fullStr Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana
title_full_unstemmed Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana
title_sort transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in nicotiana benthamiana
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://eprints.um.edu.my/20589/
https://doi.org/10.17576/jsm-2018-4703-09
_version_ 1643691323640774656
score 13.211869

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