Cover Image

Determination of sofosbuvir and velpatasvir in solid pharmaceutical dosage form by hplc-pda and its application to in vitro dissolution studies

In the present study, a newly developed method based on high performance liquid chromatography (HPLC) was optimized for the simultaneous determination of sofosbuvir (SOFOS) and velpatasvir (VELPA) in commercial tablet formulations. Isocratic separation of SOFOS and VELPA was performed at 40 ºC with...

Full description

Saved in:
Bibliographic Details
Main Authors: Tahir, Muhammad Saqlain, Ahmed, Mahmood, Qadir, Muhammad Abdul, Adnan, Ahmad, Syed, Quratulain, Low, Kah Hin, Ikram, Rabia
Format: Article
Published: Colegio de Farmaceuticos de la Provincia de Buenos Aires 2018
Subjects:
Q Science (General)
QD Chemistry
Online Access:http://eprints.um.edu.my/20579/
http://www.latamjpharm.org/resumenes/37/6/LAJOP_37_6_1_1.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.20579
record_format eprints
spelling my.um.eprints.205792019-03-04T06:19:10Z http://eprints.um.edu.my/20579/ Determination of sofosbuvir and velpatasvir in solid pharmaceutical dosage form by hplc-pda and its application to in vitro dissolution studies Tahir, Muhammad Saqlain Ahmed, Mahmood Qadir, Muhammad Abdul Adnan, Ahmad Syed, Quratulain Low, Kah Hin Ikram, Rabia Q Science (General) QD Chemistry In the present study, a newly developed method based on high performance liquid chromatography (HPLC) was optimized for the simultaneous determination of sofosbuvir (SOFOS) and velpatasvir (VELPA) in commercial tablet formulations. Isocratic separation of SOFOS and VELPA was performed at 40 ºC with Purospher Star C18 column (5 μm, 4.6 × 250 mm) at a flow rate of 1.0 mL/min whereas the mobile phase consisted of aceto nitrile, phosphate buffer and methanol (60:30:10 v/v, pH 3.0). Both analytes were detected at a wavelength of 262 nm and the injection volume was 5.0 μL. The overall run time per sample was 7.0 min with retention time of 3.251 and 4.512 min for SOFOS and VELPA, respectively. The calibration curve was linear from 10.0-70 μg/mL for SOFOS and 5.0-35.0 μg/mL for VEL-PA with a coefficient of determination equal to 0.9999 while repeatability and reproducibility (expressed as relative standard deviation) were ≤ 1.32 and 1.61 %, respectively. The proposed HPLC method was rapid and simple for the determination of SOFOS and VELPA in commercially available tablet formulations providing recoveries ≥ 99.4 % and also for dissolution studies. Colegio de Farmaceuticos de la Provincia de Buenos Aires 2018 Article PeerReviewed Tahir, Muhammad Saqlain and Ahmed, Mahmood and Qadir, Muhammad Abdul and Adnan, Ahmad and Syed, Quratulain and Low, Kah Hin and Ikram, Rabia (2018) Determination of sofosbuvir and velpatasvir in solid pharmaceutical dosage form by hplc-pda and its application to in vitro dissolution studies. Latin American Journal of Pharmacy, 37 (6). pp. 1067-1073. ISSN 0326-2383 http://www.latamjpharm.org/resumenes/37/6/LAJOP_37_6_1_1.pdf
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QD Chemistry
spellingShingle Q Science (General)
QD Chemistry
Tahir, Muhammad Saqlain
Ahmed, Mahmood
Qadir, Muhammad Abdul
Adnan, Ahmad
Syed, Quratulain
Low, Kah Hin
Ikram, Rabia
Determination of sofosbuvir and velpatasvir in solid pharmaceutical dosage form by hplc-pda and its application to in vitro dissolution studies
description In the present study, a newly developed method based on high performance liquid chromatography (HPLC) was optimized for the simultaneous determination of sofosbuvir (SOFOS) and velpatasvir (VELPA) in commercial tablet formulations. Isocratic separation of SOFOS and VELPA was performed at 40 ºC with Purospher Star C18 column (5 μm, 4.6 × 250 mm) at a flow rate of 1.0 mL/min whereas the mobile phase consisted of aceto nitrile, phosphate buffer and methanol (60:30:10 v/v, pH 3.0). Both analytes were detected at a wavelength of 262 nm and the injection volume was 5.0 μL. The overall run time per sample was 7.0 min with retention time of 3.251 and 4.512 min for SOFOS and VELPA, respectively. The calibration curve was linear from 10.0-70 μg/mL for SOFOS and 5.0-35.0 μg/mL for VEL-PA with a coefficient of determination equal to 0.9999 while repeatability and reproducibility (expressed as relative standard deviation) were ≤ 1.32 and 1.61 %, respectively. The proposed HPLC method was rapid and simple for the determination of SOFOS and VELPA in commercially available tablet formulations providing recoveries ≥ 99.4 % and also for dissolution studies.
format Article
author Tahir, Muhammad Saqlain
Ahmed, Mahmood
Qadir, Muhammad Abdul
Adnan, Ahmad
Syed, Quratulain
Low, Kah Hin
Ikram, Rabia
author_facet Tahir, Muhammad Saqlain
Ahmed, Mahmood
Qadir, Muhammad Abdul
Adnan, Ahmad
Syed, Quratulain
Low, Kah Hin
Ikram, Rabia
author_sort Tahir, Muhammad Saqlain
title Determination of sofosbuvir and velpatasvir in solid pharmaceutical dosage form by hplc-pda and its application to in vitro dissolution studies
title_short Determination of sofosbuvir and velpatasvir in solid pharmaceutical dosage form by hplc-pda and its application to in vitro dissolution studies
title_full Determination of sofosbuvir and velpatasvir in solid pharmaceutical dosage form by hplc-pda and its application to in vitro dissolution studies
title_fullStr Determination of sofosbuvir and velpatasvir in solid pharmaceutical dosage form by hplc-pda and its application to in vitro dissolution studies
title_full_unstemmed Determination of sofosbuvir and velpatasvir in solid pharmaceutical dosage form by hplc-pda and its application to in vitro dissolution studies
title_sort determination of sofosbuvir and velpatasvir in solid pharmaceutical dosage form by hplc-pda and its application to in vitro dissolution studies
publisher Colegio de Farmaceuticos de la Provincia de Buenos Aires
publishDate 2018
url http://eprints.um.edu.my/20579/
http://www.latamjpharm.org/resumenes/37/6/LAJOP_37_6_1_1.pdf
_version_ 1643691320744607744
score 13.211869

Similar Items