Staff View: Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli

Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli

This study investigated the bactericidal mechanism of peppermint essential oil (PEO) when used singly and in combination with meropenem against multidrug resistant Escherichia coli. Chemical compositions of PEO were identified via GC-MS, followed by time-kill analysis which was performed to evaluate...

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Main Authors:	Yang, Shun Kai, Yap, Polly Soo Xi, Krishnan, Thiba, Yusoff, Khatijah, Chan, Kok Gan, Yap, Wai Sum, Lai, Kok Song, Lim, Swee Hua Erin
Format:	Article
Published:	ACG Publications 2018
Subjects:	Q Science (General) QH Natural history
Online Access:	http://eprints.um.edu.my/20321/ https://doi.org/10.25135/rnp.59.17.12.078
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spelling	my.um.eprints.203212019-02-14T08:14:12Z http://eprints.um.edu.my/20321/ Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli Yang, Shun Kai Yap, Polly Soo Xi Krishnan, Thiba Yusoff, Khatijah Chan, Kok Gan Yap, Wai Sum Lai, Kok Song Lim, Swee Hua Erin Q Science (General) QH Natural history This study investigated the bactericidal mechanism of peppermint essential oil (PEO) when used singly and in combination with meropenem against multidrug resistant Escherichia coli. Chemical compositions of PEO were identified via GC-MS, followed by time-kill analysis which was performed to evaluate the antibacterial activities of PEO and meropenem. Furthermore, outer membrane permeability test, zeta potential measurement and scanning electron microscopy were performed to evaluate the ability of PEO in bacterial membrane disruption. Next, anti-quorum sensing assay was performed to assess the ability of PEO in quorum sensing inhibition. A complete killing activity was observed within five minutes of treatment with PEO and meropenem at sub-lethal concentrations. In addition, the outer membrane permeability test and zeta potential measurement performed indicated increase in the membrane permeability and membrane disruption which can be observed in the scanning electron micrograph. Furthermore, significant decrease in the light production of E. coli pSB1075 treated by PEO indicates the presence of quorum sensing inhibitors within PEO. The findings suggest that PEO has the ability to disrupt the bacterial outer membrane which increases membrane permeability, in addition to the possible inhibition of bacterial quorum sensing ability in multidrug resistant E. coli, aiding in the reversal of antibiotic resistance. ACG Publications 2018 Article PeerReviewed Yang, Shun Kai and Yap, Polly Soo Xi and Krishnan, Thiba and Yusoff, Khatijah and Chan, Kok Gan and Yap, Wai Sum and Lai, Kok Song and Lim, Swee Hua Erin (2018) Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli. Records of Natural Products, 12 (6). pp. 582-594. ISSN 1307-6167 https://doi.org/10.25135/rnp.59.17.12.078 doi:10.25135/rnp.59.17.12.078
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QH Natural history
spellingShingle	Q Science (General) QH Natural history Yang, Shun Kai Yap, Polly Soo Xi Krishnan, Thiba Yusoff, Khatijah Chan, Kok Gan Yap, Wai Sum Lai, Kok Song Lim, Swee Hua Erin Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli
description	This study investigated the bactericidal mechanism of peppermint essential oil (PEO) when used singly and in combination with meropenem against multidrug resistant Escherichia coli. Chemical compositions of PEO were identified via GC-MS, followed by time-kill analysis which was performed to evaluate the antibacterial activities of PEO and meropenem. Furthermore, outer membrane permeability test, zeta potential measurement and scanning electron microscopy were performed to evaluate the ability of PEO in bacterial membrane disruption. Next, anti-quorum sensing assay was performed to assess the ability of PEO in quorum sensing inhibition. A complete killing activity was observed within five minutes of treatment with PEO and meropenem at sub-lethal concentrations. In addition, the outer membrane permeability test and zeta potential measurement performed indicated increase in the membrane permeability and membrane disruption which can be observed in the scanning electron micrograph. Furthermore, significant decrease in the light production of E. coli pSB1075 treated by PEO indicates the presence of quorum sensing inhibitors within PEO. The findings suggest that PEO has the ability to disrupt the bacterial outer membrane which increases membrane permeability, in addition to the possible inhibition of bacterial quorum sensing ability in multidrug resistant E. coli, aiding in the reversal of antibiotic resistance.
format	Article
author	Yang, Shun Kai Yap, Polly Soo Xi Krishnan, Thiba Yusoff, Khatijah Chan, Kok Gan Yap, Wai Sum Lai, Kok Song Lim, Swee Hua Erin
author_facet	Yang, Shun Kai Yap, Polly Soo Xi Krishnan, Thiba Yusoff, Khatijah Chan, Kok Gan Yap, Wai Sum Lai, Kok Song Lim, Swee Hua Erin
author_sort	Yang, Shun Kai
title	Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli
title_short	Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli
title_full	Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli
title_fullStr	Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli
title_full_unstemmed	Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli
title_sort	mode of action: synergistic interaction of peppermint (mentha x piperita l. carl) essential oil and meropenem against plasmid-mediated resistant e. coli
publisher	ACG Publications
publishDate	2018
url	http://eprints.um.edu.my/20321/ https://doi.org/10.25135/rnp.59.17.12.078
_version_	1643691244859162624
score	13.211869

Mode of Action: Synergistic Interaction of Peppermint (Mentha x piperita L. Carl) Essential Oil and Meropenem Against Plasmid-Mediated Resistant E. coli

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