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Electrospin-coating of nitrocellulose membrane enhances sensitivity in nucleic acid-based lateral flow assay

Point-of-care biosensors are important tools developed to aid medical diagnosis and testing, food safety and environmental monitoring. Paper-based biosensors, especially nucleic acid-based lateral flow assays (LFA), are affordable, simple to produce and easy to use in remote settings. However, the s...

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Main Authors: Yew, Chee Hong, Azari, Pedram, Choi, Jane Ru, Li, Fei, Pingguan-Murphy, Belinda
Format: Article
Published: Elsevier 2018
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/20274/
https://doi.org/10.1016/j.aca.2018.01.016
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spelling my.um.eprints.202742019-02-12T08:33:11Z http://eprints.um.edu.my/20274/ Electrospin-coating of nitrocellulose membrane enhances sensitivity in nucleic acid-based lateral flow assay Yew, Chee Hong Azari, Pedram Choi, Jane Ru Li, Fei Pingguan-Murphy, Belinda R Medicine Point-of-care biosensors are important tools developed to aid medical diagnosis and testing, food safety and environmental monitoring. Paper-based biosensors, especially nucleic acid-based lateral flow assays (LFA), are affordable, simple to produce and easy to use in remote settings. However, the sensitivity of such assays to infectious diseases has always been a restrictive challenge. Here, we have successfully electrospun polycaprolactone (PCL) on nitrocellulose (NC) membrane to form a hydrophobic coating to reduce the flow rate and increase the interaction rate between the targets and gold nanoparticles-detecting probes conjugates, resulting in the binding of more complexes to the capture probes. With this approach, the sensitivity of the PCL electrospin-coated test strip has been increased by approximately ten-fold as compared to the unmodified test strip. As a proof of concept, this approach holds great potential for sensitive detection of targets at point-of-care testing. Elsevier 2018 Article PeerReviewed Yew, Chee Hong and Azari, Pedram and Choi, Jane Ru and Li, Fei and Pingguan-Murphy, Belinda (2018) Electrospin-coating of nitrocellulose membrane enhances sensitivity in nucleic acid-based lateral flow assay. Analytica Chimica Acta, 1009. pp. 81-88. ISSN 0003-2670 https://doi.org/10.1016/j.aca.2018.01.016 doi:10.1016/j.aca.2018.01.016
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Yew, Chee Hong
Azari, Pedram
Choi, Jane Ru
Li, Fei
Pingguan-Murphy, Belinda
Electrospin-coating of nitrocellulose membrane enhances sensitivity in nucleic acid-based lateral flow assay
description Point-of-care biosensors are important tools developed to aid medical diagnosis and testing, food safety and environmental monitoring. Paper-based biosensors, especially nucleic acid-based lateral flow assays (LFA), are affordable, simple to produce and easy to use in remote settings. However, the sensitivity of such assays to infectious diseases has always been a restrictive challenge. Here, we have successfully electrospun polycaprolactone (PCL) on nitrocellulose (NC) membrane to form a hydrophobic coating to reduce the flow rate and increase the interaction rate between the targets and gold nanoparticles-detecting probes conjugates, resulting in the binding of more complexes to the capture probes. With this approach, the sensitivity of the PCL electrospin-coated test strip has been increased by approximately ten-fold as compared to the unmodified test strip. As a proof of concept, this approach holds great potential for sensitive detection of targets at point-of-care testing.
format Article
author Yew, Chee Hong
Azari, Pedram
Choi, Jane Ru
Li, Fei
Pingguan-Murphy, Belinda
author_facet Yew, Chee Hong
Azari, Pedram
Choi, Jane Ru
Li, Fei
Pingguan-Murphy, Belinda
author_sort Yew, Chee Hong
title Electrospin-coating of nitrocellulose membrane enhances sensitivity in nucleic acid-based lateral flow assay
title_short Electrospin-coating of nitrocellulose membrane enhances sensitivity in nucleic acid-based lateral flow assay
title_full Electrospin-coating of nitrocellulose membrane enhances sensitivity in nucleic acid-based lateral flow assay
title_fullStr Electrospin-coating of nitrocellulose membrane enhances sensitivity in nucleic acid-based lateral flow assay
title_full_unstemmed Electrospin-coating of nitrocellulose membrane enhances sensitivity in nucleic acid-based lateral flow assay
title_sort electrospin-coating of nitrocellulose membrane enhances sensitivity in nucleic acid-based lateral flow assay
publisher Elsevier
publishDate 2018
url http://eprints.um.edu.my/20274/
https://doi.org/10.1016/j.aca.2018.01.016
_version_ 1643691231477235712
score 13.211869

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