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Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route

Natural biopolymers have many attractive medical applications; however, complications due to fibrosis caused a reduction in diffusion and dispersal of nutrients and waste products. Consequently, severe immunocompatibility problems and poor mechanical and degradation properties in synthetic polymers...

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Main Authors: Latfi, Ahmad Safwan Ahmad, Pramanik, Sumit, Poon, Chi Tat, Gumel, Ahmad Mohammed, Lai, Khin Wee, Annuar, Mohamad Suffian Mohamad, Pingguan-Murphy, Belinda
Format: Article
Published: SAGE Publications 2019
Subjects:
Q Science (General)
QH Natural history
R Medicine
Online Access:http://eprints.um.edu.my/20041/
https://doi.org/10.1177/0885328218812490
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spelling my.um.eprints.200412019-02-27T00:45:51Z http://eprints.um.edu.my/20041/ Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route Latfi, Ahmad Safwan Ahmad Pramanik, Sumit Poon, Chi Tat Gumel, Ahmad Mohammed Lai, Khin Wee Annuar, Mohamad Suffian Mohamad Pingguan-Murphy, Belinda Q Science (General) QH Natural history R Medicine Natural biopolymers have many attractive medical applications; however, complications due to fibrosis caused a reduction in diffusion and dispersal of nutrients and waste products. Consequently, severe immunocompatibility problems and poor mechanical and degradation properties in synthetic polymers ensue. Hence, the present study investigates a novel hydrogel material synthesized from caprolactone, ethylene glycol, ethylenediamine, polyethylene glycol, ammonium persulfate, and tetramethylethylenediamine via chemo-enzymatic route. Spectroscopic analyses indicated the formation of polyurea and polyhydroxyurethane as the primary building block of the hydrogel starting material. Biocompatibility studies showed positive observation in biosafety test using direct contact cytotoxicity assay in addition to active cellular growth on the hydrogel scaffold based on fluorescence observation. The synthesized hydrogel also exhibited (self)fluorescence properties under specific wavelength excitation. Hence, synthesized hydrogel could be a potential candidate for medical imaging as well as tissue engineering applications as a tissue expander, coating material, biosensor, and drug delivery system. SAGE Publications 2019 Article PeerReviewed Latfi, Ahmad Safwan Ahmad and Pramanik, Sumit and Poon, Chi Tat and Gumel, Ahmad Mohammed and Lai, Khin Wee and Annuar, Mohamad Suffian Mohamad and Pingguan-Murphy, Belinda (2019) Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route. Journal of Biomaterials Applications, 33 (6). pp. 854-865. ISSN 0885-3282 https://doi.org/10.1177/0885328218812490 doi:10.1177/0885328218812490
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QH Natural history
R Medicine
spellingShingle Q Science (General)
QH Natural history
R Medicine
Latfi, Ahmad Safwan Ahmad
Pramanik, Sumit
Poon, Chi Tat
Gumel, Ahmad Mohammed
Lai, Khin Wee
Annuar, Mohamad Suffian Mohamad
Pingguan-Murphy, Belinda
Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route
description Natural biopolymers have many attractive medical applications; however, complications due to fibrosis caused a reduction in diffusion and dispersal of nutrients and waste products. Consequently, severe immunocompatibility problems and poor mechanical and degradation properties in synthetic polymers ensue. Hence, the present study investigates a novel hydrogel material synthesized from caprolactone, ethylene glycol, ethylenediamine, polyethylene glycol, ammonium persulfate, and tetramethylethylenediamine via chemo-enzymatic route. Spectroscopic analyses indicated the formation of polyurea and polyhydroxyurethane as the primary building block of the hydrogel starting material. Biocompatibility studies showed positive observation in biosafety test using direct contact cytotoxicity assay in addition to active cellular growth on the hydrogel scaffold based on fluorescence observation. The synthesized hydrogel also exhibited (self)fluorescence properties under specific wavelength excitation. Hence, synthesized hydrogel could be a potential candidate for medical imaging as well as tissue engineering applications as a tissue expander, coating material, biosensor, and drug delivery system.
format Article
author Latfi, Ahmad Safwan Ahmad
Pramanik, Sumit
Poon, Chi Tat
Gumel, Ahmad Mohammed
Lai, Khin Wee
Annuar, Mohamad Suffian Mohamad
Pingguan-Murphy, Belinda
author_facet Latfi, Ahmad Safwan Ahmad
Pramanik, Sumit
Poon, Chi Tat
Gumel, Ahmad Mohammed
Lai, Khin Wee
Annuar, Mohamad Suffian Mohamad
Pingguan-Murphy, Belinda
author_sort Latfi, Ahmad Safwan Ahmad
title Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route
title_short Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route
title_full Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route
title_fullStr Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route
title_full_unstemmed Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route
title_sort structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route
publisher SAGE Publications
publishDate 2019
url http://eprints.um.edu.my/20041/
https://doi.org/10.1177/0885328218812490
_version_ 1643691161616908288
score 13.211869

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