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Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil.

Introduction: Plectranthus amboinicus(Lour) Spreng is a known medicinal plant used in Siddha and Ayurveda medicines in India. It has enormous medicinal potential to treat various diseases. Methods: The present study focused on the use of essential oil obtained from the leaves of Plectranthus amboini...

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Main Authors: Thirugnanasampandan, R., Ramya, G., Gogulramnath, M., Rajarajeswaran, J., Kanthimathi, M.S.
Format: Article
Published: Emanuscript Services 2014
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R Medicine
Online Access:http://eprints.um.edu.my/19550/
http://dx.doi.org/10.5530/pj.2015.7.3
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spelling my.um.eprints.195502018-10-03T03:25:20Z http://eprints.um.edu.my/19550/ Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil. Thirugnanasampandan, R. Ramya, G. Gogulramnath, M. Rajarajeswaran, J. Kanthimathi, M.S. R Medicine Introduction: Plectranthus amboinicus(Lour) Spreng is a known medicinal plant used in Siddha and Ayurveda medicines in India. It has enormous medicinal potential to treat various diseases. Methods: The present study focused on the use of essential oil obtained from the leaves of Plectranthus amboinicus to test cytotoxicity against breast (MCF-7) and colorectal (HT-29) cancer cell lines, to protect DNA from H2O2 induced genotoxicity through comet assay and to treat inflammation in lipopolysaccharide (LPS) induced over expression of matrix metalloproteinase-9 (MMP-9) in human peripheral blood mononuclear cells (PBMCs) by gelatin zymogram and inhibition at transcriptional level confirmed using RT-PCR (reverse transcriptase polymerase chain reaction). Results: Cytotoxicity of essential oil against MCF-7 and HT-29 cancer cell lines revealed the IC50 values of 53±0.01 and 87±0.01μg/mL respectively. At 200μg/mL essential oil protected against 75% of DNA damage in 3T3-L1 fibroblast cells. Essential oil showed significant reduction in production of MMP-9 in a concentration dependent manner. Conclusion: Overall, the results showed that essential oil of P. amboinicus is a potent bioactive substance and it could be used in herbal medicine preparations. Emanuscript Services 2014 Article PeerReviewed Thirugnanasampandan, R. and Ramya, G. and Gogulramnath, M. and Rajarajeswaran, J. and Kanthimathi, M.S. (2014) Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil. Pharmacognosy Journal, 7 (1). pp. 32-36. ISSN 0975-3575 http://dx.doi.org/10.5530/pj.2015.7.3 doi:10.5530/pj.2015.7.3
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Thirugnanasampandan, R.
Ramya, G.
Gogulramnath, M.
Rajarajeswaran, J.
Kanthimathi, M.S.
Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil.
description Introduction: Plectranthus amboinicus(Lour) Spreng is a known medicinal plant used in Siddha and Ayurveda medicines in India. It has enormous medicinal potential to treat various diseases. Methods: The present study focused on the use of essential oil obtained from the leaves of Plectranthus amboinicus to test cytotoxicity against breast (MCF-7) and colorectal (HT-29) cancer cell lines, to protect DNA from H2O2 induced genotoxicity through comet assay and to treat inflammation in lipopolysaccharide (LPS) induced over expression of matrix metalloproteinase-9 (MMP-9) in human peripheral blood mononuclear cells (PBMCs) by gelatin zymogram and inhibition at transcriptional level confirmed using RT-PCR (reverse transcriptase polymerase chain reaction). Results: Cytotoxicity of essential oil against MCF-7 and HT-29 cancer cell lines revealed the IC50 values of 53±0.01 and 87±0.01μg/mL respectively. At 200μg/mL essential oil protected against 75% of DNA damage in 3T3-L1 fibroblast cells. Essential oil showed significant reduction in production of MMP-9 in a concentration dependent manner. Conclusion: Overall, the results showed that essential oil of P. amboinicus is a potent bioactive substance and it could be used in herbal medicine preparations.
format Article
author Thirugnanasampandan, R.
Ramya, G.
Gogulramnath, M.
Rajarajeswaran, J.
Kanthimathi, M.S.
author_facet Thirugnanasampandan, R.
Ramya, G.
Gogulramnath, M.
Rajarajeswaran, J.
Kanthimathi, M.S.
author_sort Thirugnanasampandan, R.
title Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil.
title_short Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil.
title_full Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil.
title_fullStr Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil.
title_full_unstemmed Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil.
title_sort evaluation of cytotoxic, dna protecting and lps induced mmp-9 down regulation activities of plectranthus amboinicus (lour) spreng. essential oil.
publisher Emanuscript Services
publishDate 2014
url http://eprints.um.edu.my/19550/
http://dx.doi.org/10.5530/pj.2015.7.3
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score 13.211869

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