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Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae)

Vindogentianine, a new indole alkaloid together with six known alkaloids, vindoline, vindolidine, vindolicine, vindolinine, perivine and serpentine were isolated from leaf extract (DA) of Catharanthus roseus (L.) G. Don. Their structures were elucidated by spectroscopic methods; NMR, MS, UV and IR....

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Main Authors: Tiong, S.H., Looi, C.Y., Arya, Aditya, Wong, Won Fen, Hazni, Hazrina, Mustafa, Mohd Rais, Awang, Khalijah
Format: Article
Published: Elsevier 2015
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/19539/
http://dx.doi.org/10.1016/j.fitote.2015.01.019
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spelling my.um.eprints.195392019-03-01T09:24:34Z http://eprints.um.edu.my/19539/ Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae) Tiong, S.H. Looi, C.Y. Arya, Aditya Wong, Won Fen Hazni, Hazrina Mustafa, Mohd Rais Awang, Khalijah R Medicine Vindogentianine, a new indole alkaloid together with six known alkaloids, vindoline, vindolidine, vindolicine, vindolinine, perivine and serpentine were isolated from leaf extract (DA) of Catharanthus roseus (L.) G. Don. Their structures were elucidated by spectroscopic methods; NMR, MS, UV and IR. Vindogentianine is a dimer containing a vindoline moiety coupled to a gentianine moiety. After 24 h incubation, vindogentianine exhibited no cytotoxic effect in C2C12 mouse myoblast and β-TC6 mouse pancreatic cells (IC50 > 50 μg/mL). Real-time cell proliferation monitoring also indicated vindogentianine had little or no effect on C2C12 mouse myoblast cell growth at the highest dose tested (200 μg/mL), without inducing cell death. Vindogentianine exhibited potential hypoglycemic activity in β-TC6 and C2C12 cells by inducing higher glucose uptake and significant in vitro PTP-1B inhibition. However, in vitro α-amylase and α-glucosidase inhibition assay showed low inhibition under treatment of vindogentianine. This suggests that hypoglycemic activity of vindogentianine may be due to the enhancement of glucose uptake and PTP-1B inhibition, implying its therapeutic potential against type 2 diabetes. Elsevier 2015 Article PeerReviewed Tiong, S.H. and Looi, C.Y. and Arya, Aditya and Wong, Won Fen and Hazni, Hazrina and Mustafa, Mohd Rais and Awang, Khalijah (2015) Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae). Fitoterapia, 102. pp. 182-188. ISSN 0367-326X http://dx.doi.org/10.1016/j.fitote.2015.01.019 doi:10.1016/j.fitote.2015.01.019
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Tiong, S.H.
Looi, C.Y.
Arya, Aditya
Wong, Won Fen
Hazni, Hazrina
Mustafa, Mohd Rais
Awang, Khalijah
Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae)
description Vindogentianine, a new indole alkaloid together with six known alkaloids, vindoline, vindolidine, vindolicine, vindolinine, perivine and serpentine were isolated from leaf extract (DA) of Catharanthus roseus (L.) G. Don. Their structures were elucidated by spectroscopic methods; NMR, MS, UV and IR. Vindogentianine is a dimer containing a vindoline moiety coupled to a gentianine moiety. After 24 h incubation, vindogentianine exhibited no cytotoxic effect in C2C12 mouse myoblast and β-TC6 mouse pancreatic cells (IC50 > 50 μg/mL). Real-time cell proliferation monitoring also indicated vindogentianine had little or no effect on C2C12 mouse myoblast cell growth at the highest dose tested (200 μg/mL), without inducing cell death. Vindogentianine exhibited potential hypoglycemic activity in β-TC6 and C2C12 cells by inducing higher glucose uptake and significant in vitro PTP-1B inhibition. However, in vitro α-amylase and α-glucosidase inhibition assay showed low inhibition under treatment of vindogentianine. This suggests that hypoglycemic activity of vindogentianine may be due to the enhancement of glucose uptake and PTP-1B inhibition, implying its therapeutic potential against type 2 diabetes.
format Article
author Tiong, S.H.
Looi, C.Y.
Arya, Aditya
Wong, Won Fen
Hazni, Hazrina
Mustafa, Mohd Rais
Awang, Khalijah
author_facet Tiong, S.H.
Looi, C.Y.
Arya, Aditya
Wong, Won Fen
Hazni, Hazrina
Mustafa, Mohd Rais
Awang, Khalijah
author_sort Tiong, S.H.
title Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae)
title_short Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae)
title_full Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae)
title_fullStr Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae)
title_full_unstemmed Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae)
title_sort vindogentianine, a hypoglycemic alkaloid from catharanthus roseus (l.) g. don (apocynaceae)
publisher Elsevier
publishDate 2015
url http://eprints.um.edu.my/19539/
http://dx.doi.org/10.1016/j.fitote.2015.01.019
_version_ 1643691018809245696
score 13.211869

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