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Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells

Cryopreservation represents an effective technique to maintain the functional properties of human adipose-derived stem cells (ASCs) and allows pooling of cells via long-term storage for clinical applications, e.g., cell-based therapies. It is crucial to reduce freezing injury during the cryopreserva...

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Main Authors: Yong, K.W., Pingguan-Murphy, Belinda, Xu, F., Wan Abas, Wan Abu Bakar, Choi, J.R., Omar, Siti Zawiah, Noor Azmi, Mat Adenan, Chua, K.H., Wan Kamarul Zaman, Wan Safwani
Format: Article
Published: Nature Publishing Group 2015
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R Medicine
Online Access:http://eprints.um.edu.my/19353/
http://dx.doi.org/10.1038/srep09596
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spelling my.um.eprints.193532019-12-16T03:36:41Z http://eprints.um.edu.my/19353/ Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells Yong, K.W. Pingguan-Murphy, Belinda Xu, F. Wan Abas, Wan Abu Bakar Choi, J.R. Omar, Siti Zawiah Noor Azmi, Mat Adenan Chua, K.H. Wan Kamarul Zaman, Wan Safwani R Medicine Cryopreservation represents an effective technique to maintain the functional properties of human adipose-derived stem cells (ASCs) and allows pooling of cells via long-term storage for clinical applications, e.g., cell-based therapies. It is crucial to reduce freezing injury during the cryopreservation process by loading the ASCs with the optimum concentration of suitable cryoprotective agents (CPAs). In this study, human ASCs were preserved for 3 months in different combinations of CPAs, including 1) 0.25 M trehalose; 2) 5% dimethylsulfoxide (DMSO); 3) 10% DMSO; 4) 5% DMSO + 20% fetal bovine serum (FBS); 5) 10% DMSO + 20% FBS; 6) 10% DMSO + 90% FBS. Interestingly, even with a reduction of DMSO to 5% and without FBS, cryopreserved ASCs maintained high cell viability comparable with standard cryomedium (10% DMSO + 90% FBS), with normal cell phenotype and proliferation rate. Cryopreserved ASCs also maintained their differentiation capability (e.g., to adipocytes, osteocytes and chondrocytes) and showed an enhanced expression level of stemness markers (e.g., NANOG, OCT-4, SOX-2 and REX-1). Our findings suggest that 5% DMSO without FBS may be an ideal CPA for an efficient long-term cryopreservation of human ASCs. These results aid in establishing standardized xeno-free long-term cryopreservation of human ASCs for clinical applications. Nature Publishing Group 2015 Article PeerReviewed Yong, K.W. and Pingguan-Murphy, Belinda and Xu, F. and Wan Abas, Wan Abu Bakar and Choi, J.R. and Omar, Siti Zawiah and Noor Azmi, Mat Adenan and Chua, K.H. and Wan Kamarul Zaman, Wan Safwani (2015) Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells. Scientific Reports, 5 (1). p. 9596. ISSN 2045-2322 http://dx.doi.org/10.1038/srep09596 doi:10.1038/srep09596
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Yong, K.W.
Pingguan-Murphy, Belinda
Xu, F.
Wan Abas, Wan Abu Bakar
Choi, J.R.
Omar, Siti Zawiah
Noor Azmi, Mat Adenan
Chua, K.H.
Wan Kamarul Zaman, Wan Safwani
Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells
description Cryopreservation represents an effective technique to maintain the functional properties of human adipose-derived stem cells (ASCs) and allows pooling of cells via long-term storage for clinical applications, e.g., cell-based therapies. It is crucial to reduce freezing injury during the cryopreservation process by loading the ASCs with the optimum concentration of suitable cryoprotective agents (CPAs). In this study, human ASCs were preserved for 3 months in different combinations of CPAs, including 1) 0.25 M trehalose; 2) 5% dimethylsulfoxide (DMSO); 3) 10% DMSO; 4) 5% DMSO + 20% fetal bovine serum (FBS); 5) 10% DMSO + 20% FBS; 6) 10% DMSO + 90% FBS. Interestingly, even with a reduction of DMSO to 5% and without FBS, cryopreserved ASCs maintained high cell viability comparable with standard cryomedium (10% DMSO + 90% FBS), with normal cell phenotype and proliferation rate. Cryopreserved ASCs also maintained their differentiation capability (e.g., to adipocytes, osteocytes and chondrocytes) and showed an enhanced expression level of stemness markers (e.g., NANOG, OCT-4, SOX-2 and REX-1). Our findings suggest that 5% DMSO without FBS may be an ideal CPA for an efficient long-term cryopreservation of human ASCs. These results aid in establishing standardized xeno-free long-term cryopreservation of human ASCs for clinical applications.
format Article
author Yong, K.W.
Pingguan-Murphy, Belinda
Xu, F.
Wan Abas, Wan Abu Bakar
Choi, J.R.
Omar, Siti Zawiah
Noor Azmi, Mat Adenan
Chua, K.H.
Wan Kamarul Zaman, Wan Safwani
author_facet Yong, K.W.
Pingguan-Murphy, Belinda
Xu, F.
Wan Abas, Wan Abu Bakar
Choi, J.R.
Omar, Siti Zawiah
Noor Azmi, Mat Adenan
Chua, K.H.
Wan Kamarul Zaman, Wan Safwani
author_sort Yong, K.W.
title Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells
title_short Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells
title_full Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells
title_fullStr Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells
title_full_unstemmed Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells
title_sort phenotypic and functional characterization of long-term cryopreserved human adipose-derived stem cells
publisher Nature Publishing Group
publishDate 2015
url http://eprints.um.edu.my/19353/
http://dx.doi.org/10.1038/srep09596
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score 13.211869

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