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First report on a novel Nigrospora sphaerica isolated from Catharanthus roseus plant with anticarcinogenic properties

This paper reports on the vinca alkaloid produced by a novel Nigrospora sphaerica isolated from Catharanthus roseus. Through liquid chromatography–mass spectrometry (LCMS), only the crude mycelia extract of this fungus was positive for determination of vinblastine. This vinca alkaloid was then purif...

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Main Authors: Ayob, F.W., Simarani, K., Abidin, N.Z., Mohamad, J.
Format: Article
Published: Wiley Open Access 2017
Subjects:
Q Science (General)
QH Natural history
Online Access:http://eprints.um.edu.my/19234/
http://dx.doi.org/10.1111/1751-7915.12603
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spelling my.um.eprints.192342018-09-18T01:48:50Z http://eprints.um.edu.my/19234/ First report on a novel Nigrospora sphaerica isolated from Catharanthus roseus plant with anticarcinogenic properties Ayob, F.W. Simarani, K. Abidin, N.Z. Mohamad, J. Q Science (General) QH Natural history This paper reports on the vinca alkaloid produced by a novel Nigrospora sphaerica isolated from Catharanthus roseus. Through liquid chromatography–mass spectrometry (LCMS), only the crude mycelia extract of this fungus was positive for determination of vinblastine. This vinca alkaloid was then purified by using high-performance liquid chromatography (HPLC) and tested for cytotoxicity activity using MTT assays. The breast cell line cancer (MDA-MB 231) was treated with a purified vinblastine which was intracellulary produced by N. sphaerica. The purified vinblastine from extracted leaf of C. roseus was used as a standard comparison. A positive result with a value of half maximal inhibitory concentration (IC50) of > 32 μg ml−1 was observed compared with standard (IC50) of 350 μg ml−1 only. It showed that a vinblastine produced by N. sphaerica has a high cytotoxicity activity even though the concentration of vinblastine produced by this endophytic fungus was only 0.868 μg ml−1. Wiley Open Access 2017 Article PeerReviewed Ayob, F.W. and Simarani, K. and Abidin, N.Z. and Mohamad, J. (2017) First report on a novel Nigrospora sphaerica isolated from Catharanthus roseus plant with anticarcinogenic properties. Microbial Biotechnology, 10 (4). pp. 926-932. ISSN 1751-7915 http://dx.doi.org/10.1111/1751-7915.12603 doi:10.1111/1751-7915.12603
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Ayob, F.W.
Simarani, K.
Abidin, N.Z.
Mohamad, J.
First report on a novel Nigrospora sphaerica isolated from Catharanthus roseus plant with anticarcinogenic properties
description This paper reports on the vinca alkaloid produced by a novel Nigrospora sphaerica isolated from Catharanthus roseus. Through liquid chromatography–mass spectrometry (LCMS), only the crude mycelia extract of this fungus was positive for determination of vinblastine. This vinca alkaloid was then purified by using high-performance liquid chromatography (HPLC) and tested for cytotoxicity activity using MTT assays. The breast cell line cancer (MDA-MB 231) was treated with a purified vinblastine which was intracellulary produced by N. sphaerica. The purified vinblastine from extracted leaf of C. roseus was used as a standard comparison. A positive result with a value of half maximal inhibitory concentration (IC50) of > 32 μg ml−1 was observed compared with standard (IC50) of 350 μg ml−1 only. It showed that a vinblastine produced by N. sphaerica has a high cytotoxicity activity even though the concentration of vinblastine produced by this endophytic fungus was only 0.868 μg ml−1.
format Article
author Ayob, F.W.
Simarani, K.
Abidin, N.Z.
Mohamad, J.
author_facet Ayob, F.W.
Simarani, K.
Abidin, N.Z.
Mohamad, J.
author_sort Ayob, F.W.
title First report on a novel Nigrospora sphaerica isolated from Catharanthus roseus plant with anticarcinogenic properties
title_short First report on a novel Nigrospora sphaerica isolated from Catharanthus roseus plant with anticarcinogenic properties
title_full First report on a novel Nigrospora sphaerica isolated from Catharanthus roseus plant with anticarcinogenic properties
title_fullStr First report on a novel Nigrospora sphaerica isolated from Catharanthus roseus plant with anticarcinogenic properties
title_full_unstemmed First report on a novel Nigrospora sphaerica isolated from Catharanthus roseus plant with anticarcinogenic properties
title_sort first report on a novel nigrospora sphaerica isolated from catharanthus roseus plant with anticarcinogenic properties
publisher Wiley Open Access
publishDate 2017
url http://eprints.um.edu.my/19234/
http://dx.doi.org/10.1111/1751-7915.12603
_version_ 1643690926862761984
score 13.211869

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