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Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates

Objective To establish a suitable method of diagnosis of visceral leishmaniasis (VL) using peripheral blood, spleen or bone marrow aspirates. Methods Peripheral blood, bone marrow and spleen aspirate samples were collected from clinically suspected VL patients (n = 26). A new PCR primer pair (MK1F/R...

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Main Authors: Khatun, M., Alam, S.M.S., Khan, A.H., Hossain, M.A., Haq, J.A., Alam Jilani, M.S., Rahman, M.T., Karim, M.M.
Format: Article
Published: Elsevier 2017
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/19136/
http://dx.doi.org/10.1016/j.apjtm.2017.08.002
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spelling my.um.eprints.191362018-09-06T01:45:56Z http://eprints.um.edu.my/19136/ Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates Khatun, M. Alam, S.M.S. Khan, A.H. Hossain, M.A. Haq, J.A. Alam Jilani, M.S. Rahman, M.T. Karim, M.M. R Medicine Objective To establish a suitable method of diagnosis of visceral leishmaniasis (VL) using peripheral blood, spleen or bone marrow aspirates. Methods Peripheral blood, bone marrow and spleen aspirate samples were collected from clinically suspected VL patients (n = 26). A new PCR primer pair (MK1F/R) was designed targeting kinetoplast mini circle DNA sequences of Leishmania donovani, and Leishmania infantum, and was used to diagnose VL along with some other established primers for VL in polymerase chain reactions. Test was validated by comparing with several other diagnostic methods. Results The designed primer set showed 100% specificity and 98% sensitivity in detecting VL using blood samples, when compared with more invasive samples: bone marrow or spleen aspirates. Conclusions The newly designed primer MK1F/R could be a better alternative for PCR based diagnosis of VL using less invasive sample, peripheral blood instead of bone marrow or spleen aspirates. Elsevier 2017 Article PeerReviewed Khatun, M. and Alam, S.M.S. and Khan, A.H. and Hossain, M.A. and Haq, J.A. and Alam Jilani, M.S. and Rahman, M.T. and Karim, M.M. (2017) Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates. Asian Pacific Journal of Tropical Medicine, 10 (8). pp. 753-759. ISSN 1995-7645 http://dx.doi.org/10.1016/j.apjtm.2017.08.002 doi:10.1016/j.apjtm.2017.08.002
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Khatun, M.
Alam, S.M.S.
Khan, A.H.
Hossain, M.A.
Haq, J.A.
Alam Jilani, M.S.
Rahman, M.T.
Karim, M.M.
Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates
description Objective To establish a suitable method of diagnosis of visceral leishmaniasis (VL) using peripheral blood, spleen or bone marrow aspirates. Methods Peripheral blood, bone marrow and spleen aspirate samples were collected from clinically suspected VL patients (n = 26). A new PCR primer pair (MK1F/R) was designed targeting kinetoplast mini circle DNA sequences of Leishmania donovani, and Leishmania infantum, and was used to diagnose VL along with some other established primers for VL in polymerase chain reactions. Test was validated by comparing with several other diagnostic methods. Results The designed primer set showed 100% specificity and 98% sensitivity in detecting VL using blood samples, when compared with more invasive samples: bone marrow or spleen aspirates. Conclusions The newly designed primer MK1F/R could be a better alternative for PCR based diagnosis of VL using less invasive sample, peripheral blood instead of bone marrow or spleen aspirates.
format Article
author Khatun, M.
Alam, S.M.S.
Khan, A.H.
Hossain, M.A.
Haq, J.A.
Alam Jilani, M.S.
Rahman, M.T.
Karim, M.M.
author_facet Khatun, M.
Alam, S.M.S.
Khan, A.H.
Hossain, M.A.
Haq, J.A.
Alam Jilani, M.S.
Rahman, M.T.
Karim, M.M.
author_sort Khatun, M.
title Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates
title_short Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates
title_full Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates
title_fullStr Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates
title_full_unstemmed Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates
title_sort novel pcr primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates
publisher Elsevier
publishDate 2017
url http://eprints.um.edu.my/19136/
http://dx.doi.org/10.1016/j.apjtm.2017.08.002
_version_ 1643690898075156480
score 13.211869

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