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Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology

Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was p...

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Main Authors: Sebayang, A.H., Masjuki, Haji Hassan, Ong, H.C., Dharma, S., Silitonga, A.S., Kusumo, F., Mahlia, T.M.I., Bahar, A.H.
Format: Article
Language:English
Published: MDPI 2017
Subjects:
TJ Mechanical engineering and machinery
Online Access:http://eprints.um.edu.my/19035/1/Optimization_of_Reducing_Sugar_Production_from_Manihot_glaziovii_Starch_Using_Response_Surface_Methodology.pdf
http://eprints.um.edu.my/19035/
http://dx.doi.org/10.3390/en10010035
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spelling my.um.eprints.190352018-10-16T05:22:30Z http://eprints.um.edu.my/19035/ Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology Sebayang, A.H. Masjuki, Haji Hassan Ong, H.C. Dharma, S. Silitonga, A.S. Kusumo, F. Mahlia, T.M.I. Bahar, A.H. TJ Mechanical engineering and machinery Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, α-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g α-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii (M. glaziovii) has great potential as bioethanol feedstock. MDPI 2017 Article PeerReviewed application/pdf en http://eprints.um.edu.my/19035/1/Optimization_of_Reducing_Sugar_Production_from_Manihot_glaziovii_Starch_Using_Response_Surface_Methodology.pdf Sebayang, A.H. and Masjuki, Haji Hassan and Ong, H.C. and Dharma, S. and Silitonga, A.S. and Kusumo, F. and Mahlia, T.M.I. and Bahar, A.H. (2017) Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology. Energies, 10 (1). p. 35. ISSN 1996-1073 http://dx.doi.org/10.3390/en10010035 doi:10.3390/en10010035
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic TJ Mechanical engineering and machinery
spellingShingle TJ Mechanical engineering and machinery
Sebayang, A.H.
Masjuki, Haji Hassan
Ong, H.C.
Dharma, S.
Silitonga, A.S.
Kusumo, F.
Mahlia, T.M.I.
Bahar, A.H.
Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology
description Bioethanol is known as a viable alternative fuel to solve both energy and environmental crises. This study used response surface methodology based on the Box-Behnken experimental design to obtain the optimum conditions for and quality of bioethanol production. Enzymatic hydrolysis optimization was performed with selected hydrolysis parameters, including substrate loading, stroke speed, α-amylase concentration and amyloglucosidase concentration. From the experiment, the resulting optimum conditions are 23.88% (w/v) substrate loading, 109.43 U/g α-amylase concentration, 65.44 U/mL amyloglucosidase concentration and 74.87 rpm stroke speed, which yielded 196.23 g/L reducing sugar. The fermentation process was also carried out, with a production value of 0.45 g ethanol/g reducing sugar, which is equivalent to 88.61% of ethanol yield after fermentation by using Saccharomyces cerevisiae (S. cerevisiae). The physical and chemical properties of the produced ethanol are within the specifications of the ASTM D4806 standard. The good quality of ethanol produced from this study indicates that Manihot glaziovii (M. glaziovii) has great potential as bioethanol feedstock.
format Article
author Sebayang, A.H.
Masjuki, Haji Hassan
Ong, H.C.
Dharma, S.
Silitonga, A.S.
Kusumo, F.
Mahlia, T.M.I.
Bahar, A.H.
author_facet Sebayang, A.H.
Masjuki, Haji Hassan
Ong, H.C.
Dharma, S.
Silitonga, A.S.
Kusumo, F.
Mahlia, T.M.I.
Bahar, A.H.
author_sort Sebayang, A.H.
title Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology
title_short Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology
title_full Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology
title_fullStr Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology
title_full_unstemmed Optimization of Reducing Sugar Production from Manihot glaziovii Starch Using Response Surface Methodology
title_sort optimization of reducing sugar production from manihot glaziovii starch using response surface methodology
publisher MDPI
publishDate 2017
url http://eprints.um.edu.my/19035/1/Optimization_of_Reducing_Sugar_Production_from_Manihot_glaziovii_Starch_Using_Response_Surface_Methodology.pdf
http://eprints.um.edu.my/19035/
http://dx.doi.org/10.3390/en10010035
_version_ 1643690867451494400
score 13.211869

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