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Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains

The biofilm-forming-capability of Helicobacter pylori has been suggested to be among factors influencing treatment outcome. However, H. pylori exhibit strain-to-strain differences in biofilm-forming-capability. Metabolomics enables the inference of spatial and temporal changes of metabolic activitie...

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Main Authors: Wong, E.H.J., Ng, C.G., Goh, K.L., Vadivelu, J., Ho, B., Loke, M.F.
Format: Article
Language:English
Published: Nature Publishing Group 2018
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/18975/1/Metabolomic_analysis_of_low_and_high_biofilm-forming_Helicobacter_pylori_strains.pdf
http://eprints.um.edu.my/18975/
http://dx.doi.org/10.1038/s41598-018-19697-0
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spelling my.um.eprints.189752018-08-07T07:42:23Z http://eprints.um.edu.my/18975/ Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains Wong, E.H.J. Ng, C.G. Goh, K.L. Vadivelu, J. Ho, B. Loke, M.F. R Medicine The biofilm-forming-capability of Helicobacter pylori has been suggested to be among factors influencing treatment outcome. However, H. pylori exhibit strain-to-strain differences in biofilm-forming-capability. Metabolomics enables the inference of spatial and temporal changes of metabolic activities during biofilm formation. Our study seeks to examine the differences in metabolome of low and high biofilm-formers using the metabolomic approach. Eight H. pylori clinical strains with different biofilm-forming-capability were chosen for metabolomic analysis. Bacterial metabolites were extracted using Bligh and Dyer method and analyzed by Liquid Chromatography/Quadrupole Time-of-Flight mass spectrometry. The data was processed and analyzed using the MassHunter Qualitative Analysis and the Mass Profiler Professional programs. Based on global metabolomic profiles, low and high biofilm-formers presented as two distinctly different groups. Interestingly, low-biofilm-formers produced more metabolites than high-biofilm-formers. Further analysis was performed to identify metabolites that differed significantly (p-value < 0.005) between low and high biofilm-formers. These metabolites include major categories of lipids and metabolites involve in prostaglandin and folate metabolism. Our findings suggest that biofilm formation in H. pylori is complex and probably driven by the bacterium' endogenous metabolism. Understanding the underlying metabolic differences between low and high biofilm-formers may enhance our current understanding of pathogenesis, extragastric survival and transmission of H. pylori infections. Nature Publishing Group 2018 Article PeerReviewed application/pdf en http://eprints.um.edu.my/18975/1/Metabolomic_analysis_of_low_and_high_biofilm-forming_Helicobacter_pylori_strains.pdf Wong, E.H.J. and Ng, C.G. and Goh, K.L. and Vadivelu, J. and Ho, B. and Loke, M.F. (2018) Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains. Scientific Reports, 8 (1). pp. 1-9. ISSN 2045-2322 http://dx.doi.org/10.1038/s41598-018-19697-0 doi:10.1038/s41598-018-19697-0
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Wong, E.H.J.
Ng, C.G.
Goh, K.L.
Vadivelu, J.
Ho, B.
Loke, M.F.
Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
description The biofilm-forming-capability of Helicobacter pylori has been suggested to be among factors influencing treatment outcome. However, H. pylori exhibit strain-to-strain differences in biofilm-forming-capability. Metabolomics enables the inference of spatial and temporal changes of metabolic activities during biofilm formation. Our study seeks to examine the differences in metabolome of low and high biofilm-formers using the metabolomic approach. Eight H. pylori clinical strains with different biofilm-forming-capability were chosen for metabolomic analysis. Bacterial metabolites were extracted using Bligh and Dyer method and analyzed by Liquid Chromatography/Quadrupole Time-of-Flight mass spectrometry. The data was processed and analyzed using the MassHunter Qualitative Analysis and the Mass Profiler Professional programs. Based on global metabolomic profiles, low and high biofilm-formers presented as two distinctly different groups. Interestingly, low-biofilm-formers produced more metabolites than high-biofilm-formers. Further analysis was performed to identify metabolites that differed significantly (p-value < 0.005) between low and high biofilm-formers. These metabolites include major categories of lipids and metabolites involve in prostaglandin and folate metabolism. Our findings suggest that biofilm formation in H. pylori is complex and probably driven by the bacterium' endogenous metabolism. Understanding the underlying metabolic differences between low and high biofilm-formers may enhance our current understanding of pathogenesis, extragastric survival and transmission of H. pylori infections.
format Article
author Wong, E.H.J.
Ng, C.G.
Goh, K.L.
Vadivelu, J.
Ho, B.
Loke, M.F.
author_facet Wong, E.H.J.
Ng, C.G.
Goh, K.L.
Vadivelu, J.
Ho, B.
Loke, M.F.
author_sort Wong, E.H.J.
title Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
title_short Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
title_full Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
title_fullStr Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
title_full_unstemmed Metabolomic analysis of low and high biofilm-forming Helicobacter pylori strains
title_sort metabolomic analysis of low and high biofilm-forming helicobacter pylori strains
publisher Nature Publishing Group
publishDate 2018
url http://eprints.um.edu.my/18975/1/Metabolomic_analysis_of_low_and_high_biofilm-forming_Helicobacter_pylori_strains.pdf
http://eprints.um.edu.my/18975/
http://dx.doi.org/10.1038/s41598-018-19697-0
_version_ 1643690849377189888
score 13.211869

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