Cover Image

Genetic variation of the mitochondrial genes, CO1 and ND5, in aedes aegypti from various regions of Peninsular Malaysia

Aedes aegypti is the primary vector of dengue viruses in many parts of the world. In peninsular Malaysia, epidemics of dengue occur more at certain sites particularly in the west central region compared to the eastern region. In this study, we determined the genetic diversity of Ae. aegypti collecte...

Full description

Saved in:
Bibliographic Details
Main Authors: Yugavathy, N., Kim-Sung, L., Joanne, S., Vythilingam, I.
Format: Article
Published: Malaysian Society for Parasitology 2016
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/18749/
https://drive.google.com/file/d/0B75lcx0mfp2OcDlrUVF6ZS1Wems/view
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.18749
record_format eprints
spelling my.um.eprints.187492018-05-28T04:42:39Z http://eprints.um.edu.my/18749/ Genetic variation of the mitochondrial genes, CO1 and ND5, in aedes aegypti from various regions of Peninsular Malaysia Yugavathy, N. Kim-Sung, L. Joanne, S. Vythilingam, I. R Medicine Aedes aegypti is the primary vector of dengue viruses in many parts of the world. In peninsular Malaysia, epidemics of dengue occur more at certain sites particularly in the west central region compared to the eastern region. In this study, we determined the genetic diversity of Ae. aegypti collected from 19 localities in 5 regions of peninsular Malaysia based on two mitochondrial DNA genes; CO1 and ND5. The total number of haplotypes obtained for CO1 and ND5 gene were 14 (C1-C14) and five (N1-N5), respectively. Haplotype genealogical network constructed using concatenated CO1-ND5 gene sequences revealed 18 haplotypes (H1-H18) that were separated into two distinct lineages. Phylogenetic analysis showed that the first lineage (C11 and C3) was more closely related to those from Brazil and France. Unique low frequencies haplotypes detected in the current sampling was closely related to those previously found in India, Pakistan and Vietnam samples, suggesting recent invasion of these haplotypes through human movement or transportation. This is the first study in Malaysia which serves as an initial preliminary phase for a much larger study throughout the country. The understanding of the population diversity of Ae. aegypti and its impact on dengue transmission will be essential for planning of effective control programmes to reduce the burden of dengue. Malaysian Society for Parasitology 2016 Article PeerReviewed Yugavathy, N. and Kim-Sung, L. and Joanne, S. and Vythilingam, I. (2016) Genetic variation of the mitochondrial genes, CO1 and ND5, in aedes aegypti from various regions of Peninsular Malaysia. Tropical Biomedicine, 33 (3). pp. 543-560. ISSN 0127-5720 https://drive.google.com/file/d/0B75lcx0mfp2OcDlrUVF6ZS1Wems/view
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Yugavathy, N.
Kim-Sung, L.
Joanne, S.
Vythilingam, I.
Genetic variation of the mitochondrial genes, CO1 and ND5, in aedes aegypti from various regions of Peninsular Malaysia
description Aedes aegypti is the primary vector of dengue viruses in many parts of the world. In peninsular Malaysia, epidemics of dengue occur more at certain sites particularly in the west central region compared to the eastern region. In this study, we determined the genetic diversity of Ae. aegypti collected from 19 localities in 5 regions of peninsular Malaysia based on two mitochondrial DNA genes; CO1 and ND5. The total number of haplotypes obtained for CO1 and ND5 gene were 14 (C1-C14) and five (N1-N5), respectively. Haplotype genealogical network constructed using concatenated CO1-ND5 gene sequences revealed 18 haplotypes (H1-H18) that were separated into two distinct lineages. Phylogenetic analysis showed that the first lineage (C11 and C3) was more closely related to those from Brazil and France. Unique low frequencies haplotypes detected in the current sampling was closely related to those previously found in India, Pakistan and Vietnam samples, suggesting recent invasion of these haplotypes through human movement or transportation. This is the first study in Malaysia which serves as an initial preliminary phase for a much larger study throughout the country. The understanding of the population diversity of Ae. aegypti and its impact on dengue transmission will be essential for planning of effective control programmes to reduce the burden of dengue.
format Article
author Yugavathy, N.
Kim-Sung, L.
Joanne, S.
Vythilingam, I.
author_facet Yugavathy, N.
Kim-Sung, L.
Joanne, S.
Vythilingam, I.
author_sort Yugavathy, N.
title Genetic variation of the mitochondrial genes, CO1 and ND5, in aedes aegypti from various regions of Peninsular Malaysia
title_short Genetic variation of the mitochondrial genes, CO1 and ND5, in aedes aegypti from various regions of Peninsular Malaysia
title_full Genetic variation of the mitochondrial genes, CO1 and ND5, in aedes aegypti from various regions of Peninsular Malaysia
title_fullStr Genetic variation of the mitochondrial genes, CO1 and ND5, in aedes aegypti from various regions of Peninsular Malaysia
title_full_unstemmed Genetic variation of the mitochondrial genes, CO1 and ND5, in aedes aegypti from various regions of Peninsular Malaysia
title_sort genetic variation of the mitochondrial genes, co1 and nd5, in aedes aegypti from various regions of peninsular malaysia
publisher Malaysian Society for Parasitology
publishDate 2016
url http://eprints.um.edu.my/18749/
https://drive.google.com/file/d/0B75lcx0mfp2OcDlrUVF6ZS1Wems/view
_version_ 1643690784948486144
score 13.211869

Similar Items