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In vitro cytotoxic potential of Swietenia macrophylla King seeds against human carcinoma cell lines

Fruits of Swietenia macrophylla King (sky fruit) have been widely used in traditional medicine to treat various diseases including hypertension and diarrhea. The cytotoxic activity of the crude ethanol extract of the seeds of S. macrophylla and its fractions was assessed against selected human cance...

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Main Authors: Goh, B.H., Kadir, A.H.
Format: Article
Language:English
Published: Academic Journals 2011
Subjects:
QD Chemistry
RS Pharmacy and materia medica
Online Access:http://eprints.um.edu.my/1848/1/In_vitro_cytotoxic_potential_of_Swietenia_macrophylla_King_seeds_against_human_carcinoma_cell_lines.pdf
http://eprints.um.edu.my/1848/
http://www.academicjournals.org/jmpr/abstracts/abstracts/abstracts2011/18April/Goh%20and%20Kadir.htm
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spelling my.um.eprints.18482011-08-01T01:12:54Z http://eprints.um.edu.my/1848/ In vitro cytotoxic potential of Swietenia macrophylla King seeds against human carcinoma cell lines Goh, B.H. Kadir, A.H. QD Chemistry RS Pharmacy and materia medica Fruits of Swietenia macrophylla King (sky fruit) have been widely used in traditional medicine to treat various diseases including hypertension and diarrhea. The cytotoxic activity of the crude ethanol extract of the seeds of S. macrophylla and its fractions was assessed against selected human cancer cell lines, namely, HCT 116 (colon carcinoma), KB (nasopharyngeal epidermoid carcinoma), Ca Ski(cervical carcinoma) and MCF-7 (breast carcinoma) by using MTT assay. The S. macrophylla ethyl acetate fraction (SMEAF) showed the most potent activity against HCT116 cell line (IC50 = 35.35 μg/ml ± 0.50 ug/ml) in a dose- and time-dependent manner and was further investigated for its possible mechanisms using flow cytometric analysis. Annexin V and PI binding of treated HCT116 cells indicated apoptosis induction by SMEAF in a dose-dependent manner. The induction of apoptosis was further confirmed both by DNA fragmentation using TUNEL assay and the externalization of phosphatidylserine using Annexin V/PI stain. The cell cycle analysis revealed a prominent increase in sub-G1 population at concentrations of 0.05 mg/ml and above. Results also showed that SMEAF induced collapse of the mitochondrial membrane potential after 24 h and caused depletion in total intracellular glutathione. The result of the present investigation is the first report on the potential anticancer activity of S. macrophylla seeds and its possible mechanisms of action on cancer cell proliferation. Academic Journals 2011-02-09 Article PeerReviewed application/pdf en http://eprints.um.edu.my/1848/1/In_vitro_cytotoxic_potential_of_Swietenia_macrophylla_King_seeds_against_human_carcinoma_cell_lines.pdf Goh, B.H. and Kadir, A.H. (2011) In vitro cytotoxic potential of Swietenia macrophylla King seeds against human carcinoma cell lines. Journal Medicinal Plants Research, 5 (8). pp. 1395-1404. ISSN 1996-0875 http://www.academicjournals.org/jmpr/abstracts/abstracts/abstracts2011/18April/Goh%20and%20Kadir.htm
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic QD Chemistry
RS Pharmacy and materia medica
spellingShingle QD Chemistry
RS Pharmacy and materia medica
Goh, B.H.
Kadir, A.H.
In vitro cytotoxic potential of Swietenia macrophylla King seeds against human carcinoma cell lines
description Fruits of Swietenia macrophylla King (sky fruit) have been widely used in traditional medicine to treat various diseases including hypertension and diarrhea. The cytotoxic activity of the crude ethanol extract of the seeds of S. macrophylla and its fractions was assessed against selected human cancer cell lines, namely, HCT 116 (colon carcinoma), KB (nasopharyngeal epidermoid carcinoma), Ca Ski(cervical carcinoma) and MCF-7 (breast carcinoma) by using MTT assay. The S. macrophylla ethyl acetate fraction (SMEAF) showed the most potent activity against HCT116 cell line (IC50 = 35.35 μg/ml ± 0.50 ug/ml) in a dose- and time-dependent manner and was further investigated for its possible mechanisms using flow cytometric analysis. Annexin V and PI binding of treated HCT116 cells indicated apoptosis induction by SMEAF in a dose-dependent manner. The induction of apoptosis was further confirmed both by DNA fragmentation using TUNEL assay and the externalization of phosphatidylserine using Annexin V/PI stain. The cell cycle analysis revealed a prominent increase in sub-G1 population at concentrations of 0.05 mg/ml and above. Results also showed that SMEAF induced collapse of the mitochondrial membrane potential after 24 h and caused depletion in total intracellular glutathione. The result of the present investigation is the first report on the potential anticancer activity of S. macrophylla seeds and its possible mechanisms of action on cancer cell proliferation.
format Article
author Goh, B.H.
Kadir, A.H.
author_facet Goh, B.H.
Kadir, A.H.
author_sort Goh, B.H.
title In vitro cytotoxic potential of Swietenia macrophylla King seeds against human carcinoma cell lines
title_short In vitro cytotoxic potential of Swietenia macrophylla King seeds against human carcinoma cell lines
title_full In vitro cytotoxic potential of Swietenia macrophylla King seeds against human carcinoma cell lines
title_fullStr In vitro cytotoxic potential of Swietenia macrophylla King seeds against human carcinoma cell lines
title_full_unstemmed In vitro cytotoxic potential of Swietenia macrophylla King seeds against human carcinoma cell lines
title_sort in vitro cytotoxic potential of swietenia macrophylla king seeds against human carcinoma cell lines
publisher Academic Journals
publishDate 2011
url http://eprints.um.edu.my/1848/1/In_vitro_cytotoxic_potential_of_Swietenia_macrophylla_King_seeds_against_human_carcinoma_cell_lines.pdf
http://eprints.um.edu.my/1848/
http://www.academicjournals.org/jmpr/abstracts/abstracts/abstracts2011/18April/Goh%20and%20Kadir.htm
_version_ 1643686786434596864
score 13.211869

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