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A lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin A1c in whole blood

An immunosensor that operates based on the principles of lateral flow was developed for direct detection of hemoglobin A1c (HbA1c) in whole blood. We utilized colloidal gold-functionalized antibodies to transduce the specific signal generated when sandwich immuno-complexes were formed on the strip i...

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Main Authors: Ang, S.H., Thevarajah, T.M., Woi, P.M., Alias, Y., Khor, S.M.
Format: Article
Published: Elsevier 2016
Subjects:
QD Chemistry
R Medicine
Online Access:http://eprints.um.edu.my/18310/
https://doi.org/10.1016/j.jchromb.2016.01.059
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spelling my.um.eprints.183102017-11-17T06:34:01Z http://eprints.um.edu.my/18310/ A lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin A1c in whole blood Ang, S.H. Thevarajah, T.M. Woi, P.M. Alias, Y. Khor, S.M. QD Chemistry R Medicine An immunosensor that operates based on the principles of lateral flow was developed for direct detection of hemoglobin A1c (HbA1c) in whole blood. We utilized colloidal gold-functionalized antibodies to transduce the specific signal generated when sandwich immuno-complexes were formed on the strip in the presence of HbA1c. The number and intensity of the test lines on the strips indicate normal, under control, and elevated levels of HbA1c. In addition, a linear relationship between HbA1c levels and immunosensor signal intensity was confirmed, with a dynamic range of 4-14% (20-130 mmol mol-1) HbA1c. Using this linear relationship, we determined the HbA1c levels in blood as a function of the signal intensity on the strips. Measurements were validated using the Bio-Rad Variant II HPLC and DCA Vantage tests. Moreover, the immunosensor was verified to be highly selective for detection of HbA1c against HbA0, glycated species of HbA0, and HbA2. The limit of detection was found to be 42.5 μg mL-1 (1.35 mmol mol-1) HbA1c, which is reasonably sensitive compared to the values reported for microarray immunoassays. The shelf life of the immunosensor was estimated to be 1.4 months when stored at ambient temperature, indicating that the immunoassay is stable. Thus, the lateral flow immunosensor developed here was shown to be capable of performing selective, accurate, rapid, and stable detection of HbA1c in human blood samples. Elsevier 2016 Article PeerReviewed Ang, S.H. and Thevarajah, T.M. and Woi, P.M. and Alias, Y. and Khor, S.M. (2016) A lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin A1c in whole blood. Journal of Chromatography B, 1015. pp. 157-165. ISSN 1570-0232 https://doi.org/10.1016/j.jchromb.2016.01.059 doi:10.1016/j.jchromb.2016.01.059
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QD Chemistry
R Medicine
spellingShingle QD Chemistry
R Medicine
Ang, S.H.
Thevarajah, T.M.
Woi, P.M.
Alias, Y.
Khor, S.M.
A lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin A1c in whole blood
description An immunosensor that operates based on the principles of lateral flow was developed for direct detection of hemoglobin A1c (HbA1c) in whole blood. We utilized colloidal gold-functionalized antibodies to transduce the specific signal generated when sandwich immuno-complexes were formed on the strip in the presence of HbA1c. The number and intensity of the test lines on the strips indicate normal, under control, and elevated levels of HbA1c. In addition, a linear relationship between HbA1c levels and immunosensor signal intensity was confirmed, with a dynamic range of 4-14% (20-130 mmol mol-1) HbA1c. Using this linear relationship, we determined the HbA1c levels in blood as a function of the signal intensity on the strips. Measurements were validated using the Bio-Rad Variant II HPLC and DCA Vantage tests. Moreover, the immunosensor was verified to be highly selective for detection of HbA1c against HbA0, glycated species of HbA0, and HbA2. The limit of detection was found to be 42.5 μg mL-1 (1.35 mmol mol-1) HbA1c, which is reasonably sensitive compared to the values reported for microarray immunoassays. The shelf life of the immunosensor was estimated to be 1.4 months when stored at ambient temperature, indicating that the immunoassay is stable. Thus, the lateral flow immunosensor developed here was shown to be capable of performing selective, accurate, rapid, and stable detection of HbA1c in human blood samples.
format Article
author Ang, S.H.
Thevarajah, T.M.
Woi, P.M.
Alias, Y.
Khor, S.M.
author_facet Ang, S.H.
Thevarajah, T.M.
Woi, P.M.
Alias, Y.
Khor, S.M.
author_sort Ang, S.H.
title A lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin A1c in whole blood
title_short A lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin A1c in whole blood
title_full A lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin A1c in whole blood
title_fullStr A lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin A1c in whole blood
title_full_unstemmed A lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin A1c in whole blood
title_sort lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin a1c in whole blood
publisher Elsevier
publishDate 2016
url http://eprints.um.edu.my/18310/
https://doi.org/10.1016/j.jchromb.2016.01.059
_version_ 1643690670256291840
score 13.211869

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