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Evaluation of the routine use of the anaerobic bottle when using the BACTEC blood culture system

The established practice of sending blood cultures in an aerobic-anaerobic pair of bottles has been questioned in recent years, and this study was conducted to evaluate the routine use of an anaerobic bottle in the BACTEC blood culture set at the University of Malaya Medical Centre, Kuala Lumpur, fr...

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Main Authors: Karunakaran, R., Raja, N.S., Quek, K.F., Hoe, V.C.W., Navaratnam, P.
Format: Article
Published: Elsevier 2007
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/1782/
http://myais.fsktm.um.edu.my/3363/
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spelling my.um.eprints.17822019-09-24T08:20:25Z http://eprints.um.edu.my/1782/ Evaluation of the routine use of the anaerobic bottle when using the BACTEC blood culture system Karunakaran, R. Raja, N.S. Quek, K.F. Hoe, V.C.W. Navaratnam, P. R Medicine The established practice of sending blood cultures in an aerobic-anaerobic pair of bottles has been questioned in recent years, and this study was conducted to evaluate the routine use of an anaerobic bottle in the BACTEC blood culture set at the University of Malaya Medical Centre, Kuala Lumpur, from January to December 2004. A total of 11,663 paired blood culture sets were received, of which 3326 were from pediatric patients and 8337 were from adult patients. The overall positive isolation rate was 15%; the positive isolation rate on excluding the anaerobic bottles was 13%. Overall, there were significantly more organisms isolated from the aerobic bottle (p<0.05); however, the best yield was obtained on using the paired aerobic-anaerobic bottles. Among the positive blood culture sets, organisms were isolated from the anaerobic bottle alone in 15.2% of the pediatric sets and in 18.1% of the adult sets. Organisms that grew more frequently in the anaerobic bottle were anaerobes and some facultative anaerobes; however, the difference was not statistically significant except for anaerobes in the adult sets. We recommend that when culturing blood, an aerobic-anaerobic pair of bottles be used rather than an aerobic-aerobic pair, to optimize the recovery of a wider spectrum of organisms, including obligatory anaerobes. Elsevier 2007 Article PeerReviewed Karunakaran, R. and Raja, N.S. and Quek, K.F. and Hoe, V.C.W. and Navaratnam, P. (2007) Evaluation of the routine use of the anaerobic bottle when using the BACTEC blood culture system. Journal of Microbiology, Immunology and Infection, 40. pp. 445-449. ISSN 1684-1182 http://myais.fsktm.um.edu.my/3363/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Karunakaran, R.
Raja, N.S.
Quek, K.F.
Hoe, V.C.W.
Navaratnam, P.
Evaluation of the routine use of the anaerobic bottle when using the BACTEC blood culture system
description The established practice of sending blood cultures in an aerobic-anaerobic pair of bottles has been questioned in recent years, and this study was conducted to evaluate the routine use of an anaerobic bottle in the BACTEC blood culture set at the University of Malaya Medical Centre, Kuala Lumpur, from January to December 2004. A total of 11,663 paired blood culture sets were received, of which 3326 were from pediatric patients and 8337 were from adult patients. The overall positive isolation rate was 15%; the positive isolation rate on excluding the anaerobic bottles was 13%. Overall, there were significantly more organisms isolated from the aerobic bottle (p<0.05); however, the best yield was obtained on using the paired aerobic-anaerobic bottles. Among the positive blood culture sets, organisms were isolated from the anaerobic bottle alone in 15.2% of the pediatric sets and in 18.1% of the adult sets. Organisms that grew more frequently in the anaerobic bottle were anaerobes and some facultative anaerobes; however, the difference was not statistically significant except for anaerobes in the adult sets. We recommend that when culturing blood, an aerobic-anaerobic pair of bottles be used rather than an aerobic-aerobic pair, to optimize the recovery of a wider spectrum of organisms, including obligatory anaerobes.
format Article
author Karunakaran, R.
Raja, N.S.
Quek, K.F.
Hoe, V.C.W.
Navaratnam, P.
author_facet Karunakaran, R.
Raja, N.S.
Quek, K.F.
Hoe, V.C.W.
Navaratnam, P.
author_sort Karunakaran, R.
title Evaluation of the routine use of the anaerobic bottle when using the BACTEC blood culture system
title_short Evaluation of the routine use of the anaerobic bottle when using the BACTEC blood culture system
title_full Evaluation of the routine use of the anaerobic bottle when using the BACTEC blood culture system
title_fullStr Evaluation of the routine use of the anaerobic bottle when using the BACTEC blood culture system
title_full_unstemmed Evaluation of the routine use of the anaerobic bottle when using the BACTEC blood culture system
title_sort evaluation of the routine use of the anaerobic bottle when using the bactec blood culture system
publisher Elsevier
publishDate 2007
url http://eprints.um.edu.my/1782/
http://myais.fsktm.um.edu.my/3363/
_version_ 1646210097191321600
score 13.211869

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