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Extraction and quantification of toxic compound mimosine from leucaena leucocephala leaves

The existence of mimosine in Leucaena leucocephala prevents the utilization of its biomass residues (after energy conversion) as animal feed. In this study, mimosine quantification in Leucaena leucocephala was carried out by using rapid colorimetric method. In addition, two different extraction meth...

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Main Author: Ilham, Zul
Format: Article
Language:English
Published: Elsevier 2015
Subjects:
GE Environmental Sciences
QD Chemistry
QH301 Biology
Online Access:http://eprints.um.edu.my/16776/1/Ilhametal2015-ProChem.pdf
http://eprints.um.edu.my/16776/
http://dx.doi.org/10.1016/j.proche.2015.12.029
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spelling my.um.eprints.167762021-03-30T02:17:41Z http://eprints.um.edu.my/16776/ Extraction and quantification of toxic compound mimosine from leucaena leucocephala leaves Ilham, Zul GE Environmental Sciences QD Chemistry QH301 Biology The existence of mimosine in Leucaena leucocephala prevents the utilization of its biomass residues (after energy conversion) as animal feed. In this study, mimosine quantification in Leucaena leucocephala was carried out by using rapid colorimetric method. In addition, two different extraction methods which are soxhlet extraction with either distilled water or ethyl acetate as extraction solvent and digestion method were used to compare its efficiency in extracting the mimosine from Leucaena leucocephala leaves. The samples from both extraction methods were then clarified by boiling it with 30 mg activated carbon and filtered. The absorbance of diluted aliquot of 1-2-3-4-5 mL was then obtained from spectrophotometer at the wavelength of 535 nm. The concentration of mimosine from soxhlet extraction with distilled water was found to be higher compared to the digestion method but in contrast with ethyl acetate solvent extraction. Elsevier 2015 Article PeerReviewed application/pdf en http://eprints.um.edu.my/16776/1/Ilhametal2015-ProChem.pdf Ilham, Zul (2015) Extraction and quantification of toxic compound mimosine from leucaena leucocephala leaves. Procedia Chemistry, 16. pp. 164-170. ISSN 1876-6196 http://dx.doi.org/10.1016/j.proche.2015.12.029
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic GE Environmental Sciences
QD Chemistry
QH301 Biology
spellingShingle GE Environmental Sciences
QD Chemistry
QH301 Biology
Ilham, Zul
Extraction and quantification of toxic compound mimosine from leucaena leucocephala leaves
description The existence of mimosine in Leucaena leucocephala prevents the utilization of its biomass residues (after energy conversion) as animal feed. In this study, mimosine quantification in Leucaena leucocephala was carried out by using rapid colorimetric method. In addition, two different extraction methods which are soxhlet extraction with either distilled water or ethyl acetate as extraction solvent and digestion method were used to compare its efficiency in extracting the mimosine from Leucaena leucocephala leaves. The samples from both extraction methods were then clarified by boiling it with 30 mg activated carbon and filtered. The absorbance of diluted aliquot of 1-2-3-4-5 mL was then obtained from spectrophotometer at the wavelength of 535 nm. The concentration of mimosine from soxhlet extraction with distilled water was found to be higher compared to the digestion method but in contrast with ethyl acetate solvent extraction.
format Article
author Ilham, Zul
author_facet Ilham, Zul
author_sort Ilham, Zul
title Extraction and quantification of toxic compound mimosine from leucaena leucocephala leaves
title_short Extraction and quantification of toxic compound mimosine from leucaena leucocephala leaves
title_full Extraction and quantification of toxic compound mimosine from leucaena leucocephala leaves
title_fullStr Extraction and quantification of toxic compound mimosine from leucaena leucocephala leaves
title_full_unstemmed Extraction and quantification of toxic compound mimosine from leucaena leucocephala leaves
title_sort extraction and quantification of toxic compound mimosine from leucaena leucocephala leaves
publisher Elsevier
publishDate 2015
url http://eprints.um.edu.my/16776/1/Ilhametal2015-ProChem.pdf
http://eprints.um.edu.my/16776/
http://dx.doi.org/10.1016/j.proche.2015.12.029
_version_ 1696976579826024448
score 13.211869

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