Staff View: Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering

Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering

We developed tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu) by using the freeze-drying technique. The fabricated biocomposite scaffolds were analyzed by spectroscopy and porosity measurement. The biomechanical properties of scaffolds were assessed by compression test and th...

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Main Authors:	Puvaneswary, S., Talebian, S., Raghavendran, H.B., Murali, M.R., Mehrali, M., Afifi, A.M., Abu Kasim, N.H., Kamarul, Tunku
Format:	Article
Published:	Elsevier 2015
Subjects:	Q Science (General) QD Chemistry
Online Access:	http://eprints.um.edu.my/16213/ https://doi.org/10.1016/j.carbpol.2015.07.098
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spelling	my.um.eprints.162132018-10-10T09:08:56Z http://eprints.um.edu.my/16213/ Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering Puvaneswary, S. Talebian, S. Raghavendran, H.B. Murali, M.R. Mehrali, M. Afifi, A.M. Abu Kasim, N.H. Kamarul, Tunku Q Science (General) QD Chemistry We developed tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu) by using the freeze-drying technique. The fabricated biocomposite scaffolds were analyzed by spectroscopy and porosity measurement. The biomechanical properties of scaffolds were assessed by compression test and the results suggested that the incorporation of Fucoidan into the biocomposite improves the compression strength of scaffolds. Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion. Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold. The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture. Thus, TCP-Ch-Fu could be a potential candidate for bone-tissue engineering applications. (C) 2015 Elsevier Ltd. All rights reserved. Elsevier 2015 Article PeerReviewed Puvaneswary, S. and Talebian, S. and Raghavendran, H.B. and Murali, M.R. and Mehrali, M. and Afifi, A.M. and Abu Kasim, N.H. and Kamarul, Tunku (2015) Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering. Carbohydrate Polymers, 134. pp. 799-807. ISSN 0144-8617 https://doi.org/10.1016/j.carbpol.2015.07.098 doi:10.1016/j.carbpol.2015.07.098
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	Q Science (General) QD Chemistry
spellingShingle	Q Science (General) QD Chemistry Puvaneswary, S. Talebian, S. Raghavendran, H.B. Murali, M.R. Mehrali, M. Afifi, A.M. Abu Kasim, N.H. Kamarul, Tunku Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering
description	We developed tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu) by using the freeze-drying technique. The fabricated biocomposite scaffolds were analyzed by spectroscopy and porosity measurement. The biomechanical properties of scaffolds were assessed by compression test and the results suggested that the incorporation of Fucoidan into the biocomposite improves the compression strength of scaffolds. Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion. Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold. The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture. Thus, TCP-Ch-Fu could be a potential candidate for bone-tissue engineering applications. (C) 2015 Elsevier Ltd. All rights reserved.
format	Article
author	Puvaneswary, S. Talebian, S. Raghavendran, H.B. Murali, M.R. Mehrali, M. Afifi, A.M. Abu Kasim, N.H. Kamarul, Tunku
author_facet	Puvaneswary, S. Talebian, S. Raghavendran, H.B. Murali, M.R. Mehrali, M. Afifi, A.M. Abu Kasim, N.H. Kamarul, Tunku
author_sort	Puvaneswary, S.
title	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering
title_short	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering
title_full	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering
title_fullStr	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering
title_full_unstemmed	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering
title_sort	fabrication and in vitro biological activity of βtcp-chitosan-fucoidan composite for bone tissue engineering
publisher	Elsevier
publishDate	2015
url	http://eprints.um.edu.my/16213/ https://doi.org/10.1016/j.carbpol.2015.07.098
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score	13.211869

Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering

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