Some morphological, anatomical studies and effects of hormones on in vitro culture of canav alia rosea (sw) dc
The current investigation encompasses some morphological, anatomical and tissue culture of Canavalia rosea (Sw) DC (leguminosea). which has the potential for soil stability especially on slopes.Morphological and anatomical studies comprised leaf venation, Scanning Electron Microscopy (SEM), hist...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/15765/1/0001.pdf http://eprints.um.edu.my/15765/ |
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| Summary: | The current investigation encompasses some morphological, anatomical and
tissue culture of Canavalia rosea (Sw) DC (leguminosea). which has the
potential for soil stability especially on slopes.Morphological and anatomical
studies comprised leaf venation, Scanning Electron Microscopy (SEM),
histology analysis .and epidermal peeling. Results obtained showed that the
leaftlorsiventral with bipinate venation and amphistomic withparacytic type
stomata embedded below the epidermis. Anatomical studies of leaf and root
were also done to determine the presence of specific cells such as cuticle; oil
gland, structure of vascular system, shape and arrangement of the cells and
other related structures. Regeneration from shoots was accomplished from
leaf and stem explants within 2 months after culturing in media containing
BAP only (1.0-4.0 mg/I). High percentage of regeneration (43.75%) from
stem explants was observed on MS media supplemented with 2.0 mg/I BAP.
Roots were formed on MS media containing a combination of 1.0mg/1NAA
and 0.5mg/L BAP and on MS media with NAA only. Simultaneously, callus
were induced on MS media supplemented with BAP. Longitudinal sections
of the callus showed the existence of meristematic cells, with leaf and shoot
primordia |
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