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Aging effect and antibody immobilization on -COOH exposed surfaces designed for dengue virus detection

Polymethylmethacrylate-co-methacrylic acid, poly(MMA-co-MAA) coatings were produced with different initial molar ratios of monomers (MMA and MAA) in free-radical polymerization reaction. Polymeric platforms were specifically designed with controlled concentration of surface-exposed carboxyl (-COOH)...

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Main Authors: Hosseini, S., Ibrahim, F., Rothan, H.A., Yusof, R., van der Marel, C., Djordjevic, I., Koole, L.H.
Format: Article
Language:English
Published: Elsevier 2015
Subjects:
T Technology (General)
TA Engineering (General). Civil engineering (General)
Online Access:http://eprints.um.edu.my/15695/1/Aging_effect_and_antibody_immobilization_on_-COOH_exposed_surfaces.pdf
http://eprints.um.edu.my/15695/
http://www.sciencedirect.com/science/article/pii/S1369703X15001266
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spelling my.um.eprints.156952017-11-01T06:07:02Z http://eprints.um.edu.my/15695/ Aging effect and antibody immobilization on -COOH exposed surfaces designed for dengue virus detection Hosseini, S. Ibrahim, F. Rothan, H.A. Yusof, R. van der Marel, C. Djordjevic, I. Koole, L.H. T Technology (General) TA Engineering (General). Civil engineering (General) Polymethylmethacrylate-co-methacrylic acid, poly(MMA-co-MAA) coatings were produced with different initial molar ratios of monomers (MMA and MAA) in free-radical polymerization reaction. Polymeric platforms were specifically designed with controlled concentration of surface-exposed carboxyl (-COOH) groups that can be used as a desirable functionality for protein immobilization. Spin-coated chips were used for antibody (Ab) immobilization in order to investigate the influence of -COOH surface concentration on dengue virus detection efficiency in enzyme-linked immunosorbent assay (ELISA) experiment. Successful immobilization of Ab was achieved by two different techniques: (1) physical adsorption; and (2) covalent immobilization by carbodiimide coupling between the surface -COOH groups and amine functionalities of dengue Ab molecules. Produced polymer coatings were characterized with surface spectroscopy techniques (Raman and X-ray photoelectron spectroscopy, XPS) and water-in-air contact angle (WCA) measurements. In particular, this research concentrated on the aging effect on the availability and activity of surface -COOH groups. For that reason, WCA and Ab immobilization (ELISA) experiments were repeated on coated biochips after 3,6 and 9 months of storage. Results in this paper describe the robust and sustainable functionalized polymeric platform that can be used effectively for protein activation and development of novel biosensors. (C) 2015 Elsevier B.V. All rights reserved. Elsevier 2015-07-15 Article PeerReviewed application/pdf en http://eprints.um.edu.my/15695/1/Aging_effect_and_antibody_immobilization_on_-COOH_exposed_surfaces.pdf Hosseini, S. and Ibrahim, F. and Rothan, H.A. and Yusof, R. and van der Marel, C. and Djordjevic, I. and Koole, L.H. (2015) Aging effect and antibody immobilization on -COOH exposed surfaces designed for dengue virus detection. Biochemical Engineering Journal, 99. pp. 183-192. ISSN 1369-703X http://www.sciencedirect.com/science/article/pii/S1369703X15001266 10.1016/j.bej.2015.04.001
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic T Technology (General)
TA Engineering (General). Civil engineering (General)
spellingShingle T Technology (General)
TA Engineering (General). Civil engineering (General)
Hosseini, S.
Ibrahim, F.
Rothan, H.A.
Yusof, R.
van der Marel, C.
Djordjevic, I.
Koole, L.H.
Aging effect and antibody immobilization on -COOH exposed surfaces designed for dengue virus detection
description Polymethylmethacrylate-co-methacrylic acid, poly(MMA-co-MAA) coatings were produced with different initial molar ratios of monomers (MMA and MAA) in free-radical polymerization reaction. Polymeric platforms were specifically designed with controlled concentration of surface-exposed carboxyl (-COOH) groups that can be used as a desirable functionality for protein immobilization. Spin-coated chips were used for antibody (Ab) immobilization in order to investigate the influence of -COOH surface concentration on dengue virus detection efficiency in enzyme-linked immunosorbent assay (ELISA) experiment. Successful immobilization of Ab was achieved by two different techniques: (1) physical adsorption; and (2) covalent immobilization by carbodiimide coupling between the surface -COOH groups and amine functionalities of dengue Ab molecules. Produced polymer coatings were characterized with surface spectroscopy techniques (Raman and X-ray photoelectron spectroscopy, XPS) and water-in-air contact angle (WCA) measurements. In particular, this research concentrated on the aging effect on the availability and activity of surface -COOH groups. For that reason, WCA and Ab immobilization (ELISA) experiments were repeated on coated biochips after 3,6 and 9 months of storage. Results in this paper describe the robust and sustainable functionalized polymeric platform that can be used effectively for protein activation and development of novel biosensors. (C) 2015 Elsevier B.V. All rights reserved.
format Article
author Hosseini, S.
Ibrahim, F.
Rothan, H.A.
Yusof, R.
van der Marel, C.
Djordjevic, I.
Koole, L.H.
author_facet Hosseini, S.
Ibrahim, F.
Rothan, H.A.
Yusof, R.
van der Marel, C.
Djordjevic, I.
Koole, L.H.
author_sort Hosseini, S.
title Aging effect and antibody immobilization on -COOH exposed surfaces designed for dengue virus detection
title_short Aging effect and antibody immobilization on -COOH exposed surfaces designed for dengue virus detection
title_full Aging effect and antibody immobilization on -COOH exposed surfaces designed for dengue virus detection
title_fullStr Aging effect and antibody immobilization on -COOH exposed surfaces designed for dengue virus detection
title_full_unstemmed Aging effect and antibody immobilization on -COOH exposed surfaces designed for dengue virus detection
title_sort aging effect and antibody immobilization on -cooh exposed surfaces designed for dengue virus detection
publisher Elsevier
publishDate 2015
url http://eprints.um.edu.my/15695/1/Aging_effect_and_antibody_immobilization_on_-COOH_exposed_surfaces.pdf
http://eprints.um.edu.my/15695/
http://www.sciencedirect.com/science/article/pii/S1369703X15001266
_version_ 1643690110227578880
score 13.211869

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