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An electrochemical immunobiosensor for direct detection of veterinary drug residues in undiluted complex matrices

An electrochemical immunobiosensor is developed that allows the detection of small molecules, such as drugs, in undiluted complex samples, with no washing or rinsing steps via a displacement assay. This is achieved using an interface comprised of a mixed layer of oligo(phenylethynylene) molecular wi...

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Main Authors: Khor, S.M., Liu, G., Peterson, J.R., Iyengar, S.G., Gooding, J.J.
Format: Article
Language:English
Published: 2011
Subjects:
QD Chemistry
Online Access:http://eprints.um.edu.my/15521/1/Electroanalysis_23%282011%291797-1804_%28post-print_version%29_%28abstract%29.pdf
http://eprints.um.edu.my/15521/
http://dx.doi.org/10.1002/elan.201100205 http://onlinelibrary.wiley.com/doi/10.1002/elan.201100205/abstract
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spelling my.um.eprints.155212016-01-12T02:48:45Z http://eprints.um.edu.my/15521/ An electrochemical immunobiosensor for direct detection of veterinary drug residues in undiluted complex matrices Khor, S.M. Liu, G. Peterson, J.R. Iyengar, S.G. Gooding, J.J. QD Chemistry An electrochemical immunobiosensor is developed that allows the detection of small molecules, such as drugs, in undiluted complex samples, with no washing or rinsing steps via a displacement assay. This is achieved using an interface comprised of a mixed layer of oligo(phenylethynylene) molecular wire (MW), to allow electrochemical communication, and oligo(ethylene glycol) (OEG) to control the interaction of proteins and electroactive interferences with the electrode surface. The mixed layer is formed from in situ-generated aryl diazonium cations. To the distal end of the MW, a redox probe 1,1’-di(aminomethyl)ferrocene (FDMA) is attached followed by an epitope (the structural feature the antibody selectively recognizes) to which an antibody would bind. Association or dissociation of the antibody with the sensing interface causes a modulation of the ferrocene electrochemistry. Antibody complexed electrodes are exposed to samples containing spiked enrofloxacin (unbound target analyte), in milk and environmental water and interrogated using square wave voltammetry (SWV). The lowest detected concentration of free enrofloxacin was 10 pg/mL in phosphate buffer, 50 mM, pH 7. For free enrofloxacin detection in undiluted complex matrices, by adding disodium EDTA (50 mM), the recovery obtained was 94.1 % in skim milk and 88.5 % in stream water, respectively as compared to clean phosphate buffer. The immunobiosensor response time was 10–15 minutes. The sensor performance in milk was shown to be superior to a standard method based on Liquid Chromatography Mass Spectroscopy (LC-MS/MS). 2011 Article PeerReviewed application/pdf en http://eprints.um.edu.my/15521/1/Electroanalysis_23%282011%291797-1804_%28post-print_version%29_%28abstract%29.pdf Khor, S.M. and Liu, G. and Peterson, J.R. and Iyengar, S.G. and Gooding, J.J. (2011) An electrochemical immunobiosensor for direct detection of veterinary drug residues in undiluted complex matrices. Electroanalysis, 23 (8). pp. 1797-1804. http://dx.doi.org/10.1002/elan.201100205 http://onlinelibrary.wiley.com/doi/10.1002/elan.201100205/abstract
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Khor, S.M.
Liu, G.
Peterson, J.R.
Iyengar, S.G.
Gooding, J.J.
An electrochemical immunobiosensor for direct detection of veterinary drug residues in undiluted complex matrices
description An electrochemical immunobiosensor is developed that allows the detection of small molecules, such as drugs, in undiluted complex samples, with no washing or rinsing steps via a displacement assay. This is achieved using an interface comprised of a mixed layer of oligo(phenylethynylene) molecular wire (MW), to allow electrochemical communication, and oligo(ethylene glycol) (OEG) to control the interaction of proteins and electroactive interferences with the electrode surface. The mixed layer is formed from in situ-generated aryl diazonium cations. To the distal end of the MW, a redox probe 1,1’-di(aminomethyl)ferrocene (FDMA) is attached followed by an epitope (the structural feature the antibody selectively recognizes) to which an antibody would bind. Association or dissociation of the antibody with the sensing interface causes a modulation of the ferrocene electrochemistry. Antibody complexed electrodes are exposed to samples containing spiked enrofloxacin (unbound target analyte), in milk and environmental water and interrogated using square wave voltammetry (SWV). The lowest detected concentration of free enrofloxacin was 10 pg/mL in phosphate buffer, 50 mM, pH 7. For free enrofloxacin detection in undiluted complex matrices, by adding disodium EDTA (50 mM), the recovery obtained was 94.1 % in skim milk and 88.5 % in stream water, respectively as compared to clean phosphate buffer. The immunobiosensor response time was 10–15 minutes. The sensor performance in milk was shown to be superior to a standard method based on Liquid Chromatography Mass Spectroscopy (LC-MS/MS).
format Article
author Khor, S.M.
Liu, G.
Peterson, J.R.
Iyengar, S.G.
Gooding, J.J.
author_facet Khor, S.M.
Liu, G.
Peterson, J.R.
Iyengar, S.G.
Gooding, J.J.
author_sort Khor, S.M.
title An electrochemical immunobiosensor for direct detection of veterinary drug residues in undiluted complex matrices
title_short An electrochemical immunobiosensor for direct detection of veterinary drug residues in undiluted complex matrices
title_full An electrochemical immunobiosensor for direct detection of veterinary drug residues in undiluted complex matrices
title_fullStr An electrochemical immunobiosensor for direct detection of veterinary drug residues in undiluted complex matrices
title_full_unstemmed An electrochemical immunobiosensor for direct detection of veterinary drug residues in undiluted complex matrices
title_sort electrochemical immunobiosensor for direct detection of veterinary drug residues in undiluted complex matrices
publishDate 2011
url http://eprints.um.edu.my/15521/1/Electroanalysis_23%282011%291797-1804_%28post-print_version%29_%28abstract%29.pdf
http://eprints.um.edu.my/15521/
http://dx.doi.org/10.1002/elan.201100205 http://onlinelibrary.wiley.com/doi/10.1002/elan.201100205/abstract
_version_ 1643690073161465856
score 13.211869

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