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Development and evaluation of a multiplex polymerase chain reaction for the detection of salmonella species

The present study aims to develop a system which consists of four pairs of primers that specifically detects Salmonella spp., Salmonella serovar Typhi and Salmonella serovar Paratyphi A with an internal amplification control. The system, when applied in Polymerase Chain Reaction (PCR) under specific...

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Main Authors: Thong, Kwai Lin, Teh, C.S.J., Chua, K.H.
Format: Article
Published: Malaysian Society of Parasitology and Tropical Medicine 2014
Subjects:
Q Science (General)
Online Access:http://eprints.um.edu.my/15425/
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spelling my.um.eprints.154252019-02-13T09:11:21Z http://eprints.um.edu.my/15425/ Development and evaluation of a multiplex polymerase chain reaction for the detection of salmonella species Thong, Kwai Lin Teh, C.S.J. Chua, K.H. Q Science (General) The present study aims to develop a system which consists of four pairs of primers that specifically detects Salmonella spp., Salmonella serovar Typhi and Salmonella serovar Paratyphi A with an internal amplification control. The system, when applied in Polymerase Chain Reaction (PCR) under specific conditions, reaction mixture and cycling temperatures produced four bands; 784 bp, 496 bp, 332 bp and 187 bp. The DNA band 784 bp is present in all Salmonella spp., while the bands of 496 bp and 332 bp are only present in S. Paratyphi A and S. Typhi, respectively. An internal amplification control as indicated by the 187 bp shows the system is working in optimum condition in all the tests. This multiplex PCR was evaluated on 241 bacterial cultures and 691 naturally contaminated samples. Overall, this multiplex PCR detection system provides a single step for simultaneous detection of DNAs of Salmonella spp., S. Typhi and S. Paratyphi A. Malaysian Society of Parasitology and Tropical Medicine 2014 Article PeerReviewed Thong, Kwai Lin and Teh, C.S.J. and Chua, K.H. (2014) Development and evaluation of a multiplex polymerase chain reaction for the detection of salmonella species. Tropical Biomedicine, 31 (4). pp. 689-697. ISSN 0127-5720
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
spellingShingle Q Science (General)
Thong, Kwai Lin
Teh, C.S.J.
Chua, K.H.
Development and evaluation of a multiplex polymerase chain reaction for the detection of salmonella species
description The present study aims to develop a system which consists of four pairs of primers that specifically detects Salmonella spp., Salmonella serovar Typhi and Salmonella serovar Paratyphi A with an internal amplification control. The system, when applied in Polymerase Chain Reaction (PCR) under specific conditions, reaction mixture and cycling temperatures produced four bands; 784 bp, 496 bp, 332 bp and 187 bp. The DNA band 784 bp is present in all Salmonella spp., while the bands of 496 bp and 332 bp are only present in S. Paratyphi A and S. Typhi, respectively. An internal amplification control as indicated by the 187 bp shows the system is working in optimum condition in all the tests. This multiplex PCR was evaluated on 241 bacterial cultures and 691 naturally contaminated samples. Overall, this multiplex PCR detection system provides a single step for simultaneous detection of DNAs of Salmonella spp., S. Typhi and S. Paratyphi A.
format Article
author Thong, Kwai Lin
Teh, C.S.J.
Chua, K.H.
author_facet Thong, Kwai Lin
Teh, C.S.J.
Chua, K.H.
author_sort Thong, Kwai Lin
title Development and evaluation of a multiplex polymerase chain reaction for the detection of salmonella species
title_short Development and evaluation of a multiplex polymerase chain reaction for the detection of salmonella species
title_full Development and evaluation of a multiplex polymerase chain reaction for the detection of salmonella species
title_fullStr Development and evaluation of a multiplex polymerase chain reaction for the detection of salmonella species
title_full_unstemmed Development and evaluation of a multiplex polymerase chain reaction for the detection of salmonella species
title_sort development and evaluation of a multiplex polymerase chain reaction for the detection of salmonella species
publisher Malaysian Society of Parasitology and Tropical Medicine
publishDate 2014
url http://eprints.um.edu.my/15425/
_version_ 1643690051267198976
score 13.211869

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