Quantitative, single-step dual measurement of hemoglobin A1c and total hemoglobin in human whole blood using a gold sandwich immunochromatographic assay for personalized medicine
We describe a gold nanoparticle-based sandwich immunoassay for the dual detection and measurement of hemoglobin A1c (HbA1c) and total hemoglobin in the whole blood (without pretreatment) in a single step for personalized medicine. The optimized antibody-functionalized gold nanoparticles immunoreac...
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/15330/1/Biosensors_and_Bioelectronics_78%282016%29187-193_%28post-print_version%29.pdf http://eprints.um.edu.my/15330/ |
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| Summary: | We describe a gold nanoparticle-based sandwich immunoassay for the dual detection and measurement
of hemoglobin A1c (HbA1c) and total hemoglobin in the whole blood (without pretreatment) in a single
step for personalized medicine. The optimized antibody-functionalized gold nanoparticles immunoreact
simultaneously with HbA1c and total hemoglobin to form a sandwich at distinctive test lines to transduce
visible signals. The applicability of this method as a personal management tool was demonstrated
by establishing a calibration curve to relate % HbA1c, a useful value for type 2 diabetes management, to
the signal ratio of captured HbA1c to all other forms of hemoglobin. The platform showed excellent
selectivity (100%) toward HbA1c at distinctive test lines when challenged with HbA0, glycated HbA0 and
HbA2. The reproducibility of the measurement was good (6.02%) owing to the dual measurement of
HbA1c and total hemoglobin. A blood sample stability test revealed that the quantitative measurement of
% HbA1c was consistent and no false-positive results were detected. Also, this method distinguished the
blood sample with elevated HbF from the normal samples and the variants. The findings of this study
highlight the potential of a lateral flow immunosensor as a simple, inexpensive, consistent, and convenient
strategy for the dual measurement of HbA1c and total Hb to provide useful % HbA1c values for
better on-site diabetes care. |
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