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Comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (Dioscorea alata L)

Antioxidants are compounds that protect cells against the damaging effects of reactive oxygen species such as singlet oxygen, superoxide, peroxyl radicals, and peroxy nitrite which result in oxidative stress leading to cellular damage. Natural antioxidant are in high demand because of their potentia...

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Main Authors: Sakinah, A., Rosna, M.T.
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
Q Science (General)
QH Natural history
Online Access:http://eprints.um.edu.my/15051/1/0001.pdf
http://eprints.um.edu.my/15051/
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spelling my.um.eprints.150512015-12-14T06:21:05Z http://eprints.um.edu.my/15051/ Comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (Dioscorea alata L) Sakinah, A. Rosna, M.T. Q Science (General) QH Natural history Antioxidants are compounds that protect cells against the damaging effects of reactive oxygen species such as singlet oxygen, superoxide, peroxyl radicals, and peroxy nitrite which result in oxidative stress leading to cellular damage. Natural antioxidant are in high demand because of their potential in health promotion and disease prevention and their improved safety and consumer acceptability. Plants are rich sources of natural antioxidant. Dioscorea a/ata L. known as "ubi badak" in Malaysia were well known for their antioxidant content but this plant was seasonal. Thus, tissue culture technique was used to mass propagate this plant. In the present work, a comparative study between in vitro (from tissue culture) and in vivo (from intact plant) samples of Dioscorea alata L. for their antioxidant potential by 2,2-diphenil -1- picrylhydrazyl (DPPH) radical scavenging activity method and their total phenolic and flavonoid contents were carried out. All samples had better radical scavenging activity but in vivo samples had the strongest radical scavenging activity compared to in vitro samples. Furthermore, tubers from in vivo samples showed the greatest free radical scavenging effect and comparatively greater phenolic content than in vitro samples. 2015-11 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.um.edu.my/15051/1/0001.pdf Sakinah, A. and Rosna, M.T. (2015) Comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (Dioscorea alata L). In: 17th International Conference on Biotechnology (ICBT 2015 : 17th), 12-13 November 2015, Madrid, Spain.
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Sakinah, A.
Rosna, M.T.
Comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (Dioscorea alata L)
description Antioxidants are compounds that protect cells against the damaging effects of reactive oxygen species such as singlet oxygen, superoxide, peroxyl radicals, and peroxy nitrite which result in oxidative stress leading to cellular damage. Natural antioxidant are in high demand because of their potential in health promotion and disease prevention and their improved safety and consumer acceptability. Plants are rich sources of natural antioxidant. Dioscorea a/ata L. known as "ubi badak" in Malaysia were well known for their antioxidant content but this plant was seasonal. Thus, tissue culture technique was used to mass propagate this plant. In the present work, a comparative study between in vitro (from tissue culture) and in vivo (from intact plant) samples of Dioscorea alata L. for their antioxidant potential by 2,2-diphenil -1- picrylhydrazyl (DPPH) radical scavenging activity method and their total phenolic and flavonoid contents were carried out. All samples had better radical scavenging activity but in vivo samples had the strongest radical scavenging activity compared to in vitro samples. Furthermore, tubers from in vivo samples showed the greatest free radical scavenging effect and comparatively greater phenolic content than in vitro samples.
format Conference or Workshop Item
author Sakinah, A.
Rosna, M.T.
author_facet Sakinah, A.
Rosna, M.T.
author_sort Sakinah, A.
title Comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (Dioscorea alata L)
title_short Comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (Dioscorea alata L)
title_full Comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (Dioscorea alata L)
title_fullStr Comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (Dioscorea alata L)
title_full_unstemmed Comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (Dioscorea alata L)
title_sort comparative study of antioxidant activity in in vivo and in vitro samples of purple greater yarn (dioscorea alata l)
publishDate 2015
url http://eprints.um.edu.my/15051/1/0001.pdf
http://eprints.um.edu.my/15051/
_version_ 1643689968201105408
score 13.211869

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