Recombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B
In this study the potential of the previously contracted recombinant clone ABA392 derived from Pasteurella multocida serotype B to protect laboratory animal against haemorrhagic septicaemia was determined. After reconfirmation of plasmid DNA size, its stability and pathological effect of the clone,...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
Academic Journals
2011
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/14592/ http://www.academicjournals.org/journal/AJMR/article-abstract/16709AF12581 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my.um.eprints.14592 |
|---|---|
| record_format |
eprints |
| spelling |
my.um.eprints.145922015-11-06T01:57:00Z http://eprints.um.edu.my/14592/ Recombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B Hussaini, J. Nazmul, M.H.M. Abdullah, M.A. Ismail, S. QH Natural history R Medicine In this study the potential of the previously contracted recombinant clone ABA392 derived from Pasteurella multocida serotype B to protect laboratory animal against haemorrhagic septicaemia was determined. After reconfirmation of plasmid DNA size, its stability and pathological effect of the clone, passive mice protection test and active immunization was carried out. Pooled serum sample from rats vaccinated with live and killed form of the clone was administered to mice and provided 66% protection while active immunization with the recombinant clone conferred 83% immunity to mice when challenged with lethal dose of P. multocida. ELISA results were positive for presence of antibody in serum of immunized mice. Sub-cloning of the insert ABA392 into an expression vector pQE32 was carried out to express its protein. It was found that the recombinant clone ABA392 is immunogenic and could be used as vaccine in future. Academic Journals 2011-09 Article PeerReviewed Hussaini, J. and Nazmul, M.H.M. and Abdullah, M.A. and Ismail, S. (2011) Recombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B. African Journal of Microbiology Research, 5 (18). pp. 2596-2599. ISSN 1996-0808 http://www.academicjournals.org/journal/AJMR/article-abstract/16709AF12581 DOI: 10.5897/AJMR11.144 |
| institution |
Universiti Malaya |
| building |
UM Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Malaya |
| content_source |
UM Research Repository |
| url_provider |
http://eprints.um.edu.my/ |
| topic |
QH Natural history R Medicine |
| spellingShingle |
QH Natural history R Medicine Hussaini, J. Nazmul, M.H.M. Abdullah, M.A. Ismail, S. Recombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B |
| description |
In this study the potential of the previously contracted recombinant clone ABA392 derived from Pasteurella multocida serotype B to protect laboratory animal against haemorrhagic septicaemia was determined. After reconfirmation of plasmid DNA size, its stability and pathological effect of the clone, passive mice protection test and active immunization was carried out. Pooled serum sample from rats vaccinated with live and killed form of the clone was administered to mice and provided 66% protection while active immunization with the recombinant clone conferred 83% immunity to mice when challenged with lethal dose of P. multocida. ELISA results were positive for presence of antibody in serum of immunized mice. Sub-cloning of the insert ABA392 into an expression vector pQE32 was carried out to express its protein. It was found that the recombinant clone ABA392 is immunogenic and could be used as vaccine in future. |
| format |
Article |
| author |
Hussaini, J. Nazmul, M.H.M. Abdullah, M.A. Ismail, S. |
| author_facet |
Hussaini, J. Nazmul, M.H.M. Abdullah, M.A. Ismail, S. |
| author_sort |
Hussaini, J. |
| title |
Recombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B |
| title_short |
Recombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B |
| title_full |
Recombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B |
| title_fullStr |
Recombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B |
| title_full_unstemmed |
Recombinant Clone ABA392 protects laboratory animals from Pasteurella multocida Serotype B |
| title_sort |
recombinant clone aba392 protects laboratory animals from pasteurella multocida serotype b |
| publisher |
Academic Journals |
| publishDate |
2011 |
| url |
http://eprints.um.edu.my/14592/ http://www.academicjournals.org/journal/AJMR/article-abstract/16709AF12581 |
| _version_ |
1643689852983574528 |
| score |
13.211869 |