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Idala: An unnamed Function Peptide Vaccine for Tuberculosis

Purpose: To evaluate Myt272 protein antigenicity and immunogenicity by trial vaccination in mice and its in silico analysis as a potential peptide vaccine for tuberculosis. Methods: Myt272 gene, which has 100 % identity with Mycobacterium tuberculosis H37Rv unknown function gene Rv3424c, was liga...

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Main Authors: Ismail, Salmah, Darwish, N.T., Teoh, T.C., Abdulla, Mahmood Ameen, Omar, R.
Format: Article
Published: Pharmacotherapy Group, University of Benin, Benin City 2012
Subjects:
QH Natural history
R Medicine
Online Access:http://eprints.um.edu.my/14590/
http://www.tjpr.org/vol11_no6/2012_11_6_2.php
http://dx.doi.org/10.4314/tjpr.v11i6.2
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spelling my.um.eprints.145902019-02-13T09:00:03Z http://eprints.um.edu.my/14590/ Idala: An unnamed Function Peptide Vaccine for Tuberculosis Ismail, Salmah Darwish, N.T. Teoh, T.C. Abdulla, Mahmood Ameen Omar, R. QH Natural history R Medicine Purpose: To evaluate Myt272 protein antigenicity and immunogenicity by trial vaccination in mice and its in silico analysis as a potential peptide vaccine for tuberculosis. Methods: Myt272 gene, which has 100 % identity with Mycobacterium tuberculosis H37Rv unknown function gene Rv3424c, was ligated by genomic shotgun approach into the expression vector pQE32, and transformed into Escherichia coli SG13009. Expression during cell growth was induced by isopropyl-β-D-thiogalactopyranoside. The recombinant protein was isolated from the harvested cell lysate and injected in mice for immunogenicity experiment up to 42 days. ELISA tests with anti-His antibodies were performed on the collected individual blood samples’ sera. Color development in a microplate reader was measured at 450 nm. Results: The protein was predicted to have a mass of approximately 13 kDa and was present in the soluble fraction of the cell lysate. The immunogenicity test on Myt272 protein revealed very statistically significant high levels of antibodies detected by ELISA in the sera of immunized group of mice compared to negative controls. Conclusion: A 10.1 kD unnamed function (IDALA) protein from Rv3424 gene could be the potential peptide vaccine for tuberculosis tested by mice immunogenicity experiment. Pharmacotherapy Group, University of Benin, Benin City 2012-12 Article PeerReviewed Ismail, Salmah and Darwish, N.T. and Teoh, T.C. and Abdulla, Mahmood Ameen and Omar, R. (2012) Idala: An unnamed Function Peptide Vaccine for Tuberculosis. Tropical Journal of Pharmaceutical Research, 11 (6). pp. 879-886. ISSN 1596-5996 http://www.tjpr.org/vol11_no6/2012_11_6_2.php http://dx.doi.org/10.4314/tjpr.v11i6.2
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QH Natural history
R Medicine
spellingShingle QH Natural history
R Medicine
Ismail, Salmah
Darwish, N.T.
Teoh, T.C.
Abdulla, Mahmood Ameen
Omar, R.
Idala: An unnamed Function Peptide Vaccine for Tuberculosis
description Purpose: To evaluate Myt272 protein antigenicity and immunogenicity by trial vaccination in mice and its in silico analysis as a potential peptide vaccine for tuberculosis. Methods: Myt272 gene, which has 100 % identity with Mycobacterium tuberculosis H37Rv unknown function gene Rv3424c, was ligated by genomic shotgun approach into the expression vector pQE32, and transformed into Escherichia coli SG13009. Expression during cell growth was induced by isopropyl-β-D-thiogalactopyranoside. The recombinant protein was isolated from the harvested cell lysate and injected in mice for immunogenicity experiment up to 42 days. ELISA tests with anti-His antibodies were performed on the collected individual blood samples’ sera. Color development in a microplate reader was measured at 450 nm. Results: The protein was predicted to have a mass of approximately 13 kDa and was present in the soluble fraction of the cell lysate. The immunogenicity test on Myt272 protein revealed very statistically significant high levels of antibodies detected by ELISA in the sera of immunized group of mice compared to negative controls. Conclusion: A 10.1 kD unnamed function (IDALA) protein from Rv3424 gene could be the potential peptide vaccine for tuberculosis tested by mice immunogenicity experiment.
format Article
author Ismail, Salmah
Darwish, N.T.
Teoh, T.C.
Abdulla, Mahmood Ameen
Omar, R.
author_facet Ismail, Salmah
Darwish, N.T.
Teoh, T.C.
Abdulla, Mahmood Ameen
Omar, R.
author_sort Ismail, Salmah
title Idala: An unnamed Function Peptide Vaccine for Tuberculosis
title_short Idala: An unnamed Function Peptide Vaccine for Tuberculosis
title_full Idala: An unnamed Function Peptide Vaccine for Tuberculosis
title_fullStr Idala: An unnamed Function Peptide Vaccine for Tuberculosis
title_full_unstemmed Idala: An unnamed Function Peptide Vaccine for Tuberculosis
title_sort idala: an unnamed function peptide vaccine for tuberculosis
publisher Pharmacotherapy Group, University of Benin, Benin City
publishDate 2012
url http://eprints.um.edu.my/14590/
http://www.tjpr.org/vol11_no6/2012_11_6_2.php
http://dx.doi.org/10.4314/tjpr.v11i6.2
_version_ 1643689852401614848
score 13.211869

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