Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression
Candida albicans is an opportunistic human pathogen with the ability to differentiate and grow in filamentous forms and exist as biofilms. The biofilms are a barrier to treatment as they are often resistant to the antifungal drugs. In this study, we investigated the antifungal activity of allicin, a...
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my.um.eprints.145412019-12-23T08:06:51Z http://eprints.um.edu.my/14541/ Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression Khodavandi, A. Harmal, N.S. Alizadeh, F. Scully, O.J. Sidik, S.M. Othman, Fauziah Sekawi, Z. Ng, Kee Peng Chong, P.P. Q Science (General) R Medicine Candida albicans is an opportunistic human pathogen with the ability to differentiate and grow in filamentous forms and exist as biofilms. The biofilms are a barrier to treatment as they are often resistant to the antifungal drugs. In this study, we investigated the antifungal activity of allicin, an active compound of garlic on various isolates of C. albicans. The effect of allicin on biofilm production in C. albicans as compared to fluconazole, an antifungal drug, was investigated using the tetrazolium (XXT) reduction-dependent growth and crystal violet assays as well as scanning electron microscopy (SEM). Allicin-treated cells exhibited significant reduction in biofilm growth (p<0.05) compared to fluconazole-treated and also growth control cells. Moreover, observation by SEM of allicin and fluconazole-treated cells confirmed a dose-dependent membrane disruption and decreased production of organisms. Finally, the expression of selected genes involved in biofilm formation such as HWP1 was evaluated by semi-quantitative RT-PCR and relative real time RT-PCR. Allicin was shown to down-regulate the expression of HWP1. (C) 2011 Elsevier GmbH. All rights reserved. Elsevier 2011 Article PeerReviewed Khodavandi, A. and Harmal, N.S. and Alizadeh, F. and Scully, O.J. and Sidik, S.M. and Othman, Fauziah and Sekawi, Z. and Ng, Kee Peng and Chong, P.P. (2011) Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression. Phytomedicine, 19 (1). pp. 56-63. https://doi.org/10.1016/j.phymed.2011.08.060 |
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Q Science (General) R Medicine Khodavandi, A. Harmal, N.S. Alizadeh, F. Scully, O.J. Sidik, S.M. Othman, Fauziah Sekawi, Z. Ng, Kee Peng Chong, P.P. Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression |
| description |
Candida albicans is an opportunistic human pathogen with the ability to differentiate and grow in filamentous forms and exist as biofilms. The biofilms are a barrier to treatment as they are often resistant to the antifungal drugs. In this study, we investigated the antifungal activity of allicin, an active compound of garlic on various isolates of C. albicans. The effect of allicin on biofilm production in C. albicans as compared to fluconazole, an antifungal drug, was investigated using the tetrazolium (XXT) reduction-dependent growth and crystal violet assays as well as scanning electron microscopy (SEM). Allicin-treated cells exhibited significant reduction in biofilm growth (p<0.05) compared to fluconazole-treated and also growth control cells. Moreover, observation by SEM of allicin and fluconazole-treated cells confirmed a dose-dependent membrane disruption and decreased production of organisms. Finally, the expression of selected genes involved in biofilm formation such as HWP1 was evaluated by semi-quantitative RT-PCR and relative real time RT-PCR. Allicin was shown to down-regulate the expression of HWP1. (C) 2011 Elsevier GmbH. All rights reserved. |
| format |
Article |
| author |
Khodavandi, A. Harmal, N.S. Alizadeh, F. Scully, O.J. Sidik, S.M. Othman, Fauziah Sekawi, Z. Ng, Kee Peng Chong, P.P. |
| author_facet |
Khodavandi, A. Harmal, N.S. Alizadeh, F. Scully, O.J. Sidik, S.M. Othman, Fauziah Sekawi, Z. Ng, Kee Peng Chong, P.P. |
| author_sort |
Khodavandi, A. |
| title |
Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression |
| title_short |
Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression |
| title_full |
Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression |
| title_fullStr |
Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression |
| title_full_unstemmed |
Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression |
| title_sort |
comparison between allicin and fluconazole in candida albicans biofilm inhibition and in suppression of hwp1 gene expression |
| publisher |
Elsevier |
| publishDate |
2011 |
| url |
http://eprints.um.edu.my/14541/ https://doi.org/10.1016/j.phymed.2011.08.060 |
| _version_ |
1654960664263786496 |
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13.211869 |