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Inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection

Quorum sensing (QS) is a key regulator of virulence factors and biofilm formation in Gram-negative bacteria such as Pseudomonas aeruginosa. Microorganisms that inhabit soil are of strategic importance in the discovery of compounds with anti-QS properties. The objective of the study was to test the c...

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Main Authors: Alasil, S.M., Omar, R., Ismail, S., Yusof, M.Y.
Format: Article
Published: Hindawi Publishing Corporation 2015
Subjects:
Q Science (General)
QH Natural history
Online Access:http://eprints.um.edu.my/14505/
http://www.hindawi.com/journals/ijb/2015/671562/abs/
http://dx.doi.org/10.1155/2015/671562
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spelling my.um.eprints.145052015-12-30T03:45:07Z http://eprints.um.edu.my/14505/ Inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection Alasil, S.M. Omar, R. Ismail, S. Yusof, M.Y. Q Science (General) QH Natural history Quorum sensing (QS) is a key regulator of virulence factors and biofilm formation in Gram-negative bacteria such as Pseudomonas aeruginosa. Microorganisms that inhabit soil are of strategic importance in the discovery of compounds with anti-QS properties. The objective of the study was to test the culture extract of a taxonomically novel species of Paenibacillus strain 139SI for its inhibitory effects on the QS-controlled virulence factors and biofilm formation of Pseudomonas aeruginosa both in vitro and in vivo. The Paenibacillus sp. culture extract was used to test its anti-QS effects on the LasA protease, LasB elastase, pyoverdin production, and biofilm formation of P. aeruginosa as well as evaluate its therapeutic effects on lung bacteriology, pathology, hematological profile, and serum antibody responses of experimental animals in a rat model of chronic lung infection. Results showed significant decrease in the activities of QS-controlled LasA protease, LasB elastase pyoverdin, and biofilm formation of P. aeruginosa caused by the culture extract. Moreover, the extract significantly prolonged the survival times of rats and facilitated the clearance of biofilm infections from infected lungs. In conclusion, the antiquorum sensing effects of culture extract from a novel species of Paenibacillus provide new insights to combat biofilm-associated infections. Hindawi Publishing Corporation 2015 Article PeerReviewed Alasil, S.M. and Omar, R. and Ismail, S. and Yusof, M.Y. (2015) Inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection. International Journal of Bacteriology, 2015. pp. 1-16. ISSN 2356-6957 http://www.hindawi.com/journals/ijb/2015/671562/abs/ http://dx.doi.org/10.1155/2015/671562
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
QH Natural history
spellingShingle Q Science (General)
QH Natural history
Alasil, S.M.
Omar, R.
Ismail, S.
Yusof, M.Y.
Inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection
description Quorum sensing (QS) is a key regulator of virulence factors and biofilm formation in Gram-negative bacteria such as Pseudomonas aeruginosa. Microorganisms that inhabit soil are of strategic importance in the discovery of compounds with anti-QS properties. The objective of the study was to test the culture extract of a taxonomically novel species of Paenibacillus strain 139SI for its inhibitory effects on the QS-controlled virulence factors and biofilm formation of Pseudomonas aeruginosa both in vitro and in vivo. The Paenibacillus sp. culture extract was used to test its anti-QS effects on the LasA protease, LasB elastase, pyoverdin production, and biofilm formation of P. aeruginosa as well as evaluate its therapeutic effects on lung bacteriology, pathology, hematological profile, and serum antibody responses of experimental animals in a rat model of chronic lung infection. Results showed significant decrease in the activities of QS-controlled LasA protease, LasB elastase pyoverdin, and biofilm formation of P. aeruginosa caused by the culture extract. Moreover, the extract significantly prolonged the survival times of rats and facilitated the clearance of biofilm infections from infected lungs. In conclusion, the antiquorum sensing effects of culture extract from a novel species of Paenibacillus provide new insights to combat biofilm-associated infections.
format Article
author Alasil, S.M.
Omar, R.
Ismail, S.
Yusof, M.Y.
author_facet Alasil, S.M.
Omar, R.
Ismail, S.
Yusof, M.Y.
author_sort Alasil, S.M.
title Inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection
title_short Inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection
title_full Inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection
title_fullStr Inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection
title_full_unstemmed Inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection
title_sort inhibition of quorum sensing-controlled virulence factors and biofilm formation in pseudomonas aeruginosa by culture extract from novel bacterial species of paenibacillus using a rat model of chronic lung infection
publisher Hindawi Publishing Corporation
publishDate 2015
url http://eprints.um.edu.my/14505/
http://www.hindawi.com/journals/ijb/2015/671562/abs/
http://dx.doi.org/10.1155/2015/671562
_version_ 1643689832686288896
score 13.211869

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