Fusion PCR generated Japanese encephalitis virus/dengue 4 virus chimera exhibits lack of neuroinvasiveness, attenuated neurovirulence, and a dual-flavi immune response in mice
The first flavivirus chimera encoding dengue 4 virus (D4) PrM and E structural proteins in a Japanese encephalitis virus (JEV) backbone was successfully generated using the long-PCR based cDNA-fragment stitching (LPCRcFS) technique, demonstrating the technique's applicability for rapid preparat...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Published: |
Society for General Microbiology
2004
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/13172/ http://vir.sgmjournals.org/content/85/9/2503.long http://dx.doi.org/10.1099/vir.0.80120-0 |
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