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In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method

The in vitro susceptibility of clinical Candida isolates towards fluconazole and voriconazole was determined using the E-test method. A total of 41 clinical isolates recovered from patients since 2004 until 2009 from two local hospitals in Kuala Lumpur, Malaysia were used. These comprised Candida tr...

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Main Authors: Madhavan, P., Jamal, F., Chong, P.P., Ng, K.P.
Format: Article
Published: Malaysian Soc Parasitology Tropical Medicine 2010
Subjects:
Q Science (General)
Online Access:http://eprints.um.edu.my/12672/
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spelling my.um.eprints.126722015-02-10T07:43:01Z http://eprints.um.edu.my/12672/ In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method Madhavan, P. Jamal, F. Chong, P.P. Ng, K.P. Q Science (General) The in vitro susceptibility of clinical Candida isolates towards fluconazole and voriconazole was determined using the E-test method. A total of 41 clinical isolates recovered from patients since 2004 until 2009 from two local hospitals in Kuala Lumpur, Malaysia were used. These comprised Candida tropicalis, Candida albicans, Candida krusei, Candida parapsilosis, Candida rugosa, Candida dubliniensis and Candida glabrata. Strains from American Type Culture Collection were used as quality control. Lawn cultures of the isolates on RPMI-1640 agar medium supplemented with 2% glucose were incubated with the E-test strips at 35 degrees C for 48 h. Our results show that 71% were susceptible to fluconazole and 90% were susceptible to voriconazole. All strains of C. krusei were resistant to fluconazole and 50% were susceptible in a dose-dependent manner to voriconazole. There were 66% and 33% of C. glabrata that were resistant to fluconazole and voriconazole. Our study revealed that majority of the clinical Candida isolates was susceptible to fluconazole and voriconazole with a small percentage being resistant to both the drugs. Malaysian Soc Parasitology Tropical Medicine 2010 Article PeerReviewed Madhavan, P. and Jamal, F. and Chong, P.P. and Ng, K.P. (2010) In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method. Tropical Biomedicine, 27 (2). pp. 200-207.
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic Q Science (General)
spellingShingle Q Science (General)
Madhavan, P.
Jamal, F.
Chong, P.P.
Ng, K.P.
In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method
description The in vitro susceptibility of clinical Candida isolates towards fluconazole and voriconazole was determined using the E-test method. A total of 41 clinical isolates recovered from patients since 2004 until 2009 from two local hospitals in Kuala Lumpur, Malaysia were used. These comprised Candida tropicalis, Candida albicans, Candida krusei, Candida parapsilosis, Candida rugosa, Candida dubliniensis and Candida glabrata. Strains from American Type Culture Collection were used as quality control. Lawn cultures of the isolates on RPMI-1640 agar medium supplemented with 2% glucose were incubated with the E-test strips at 35 degrees C for 48 h. Our results show that 71% were susceptible to fluconazole and 90% were susceptible to voriconazole. All strains of C. krusei were resistant to fluconazole and 50% were susceptible in a dose-dependent manner to voriconazole. There were 66% and 33% of C. glabrata that were resistant to fluconazole and voriconazole. Our study revealed that majority of the clinical Candida isolates was susceptible to fluconazole and voriconazole with a small percentage being resistant to both the drugs.
format Article
author Madhavan, P.
Jamal, F.
Chong, P.P.
Ng, K.P.
author_facet Madhavan, P.
Jamal, F.
Chong, P.P.
Ng, K.P.
author_sort Madhavan, P.
title In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method
title_short In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method
title_full In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method
title_fullStr In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method
title_full_unstemmed In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method
title_sort in vitro activity of fluconazole and voriconazole against clinical isolates of candida spp. by e-test method
publisher Malaysian Soc Parasitology Tropical Medicine
publishDate 2010
url http://eprints.um.edu.my/12672/
_version_ 1643689350645415936
score 13.211869

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