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Adult drosophila melanogaster glutathione S-transferases: effects of acute treatment with methyl parathion

The effect of acute treatment of methyl parathion (MP) on the expression of BSP/GSH-agarose purified glutathione S-transferases (GSTs) in Drosophila melanogaster was investigated. Using 2-D gel analysis of the identified Epsilon-class, only DmGSTE6 (100%) and DmGSTE7 (72%) demonstrated significant i...

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Main Authors: Alias, Z., Clark, A.G.
Format: Article
Published: Academic Press Inc Elsevier Science 2010
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/12502/
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spelling my.um.eprints.125022015-01-30T03:06:58Z http://eprints.um.edu.my/12502/ Adult drosophila melanogaster glutathione S-transferases: effects of acute treatment with methyl parathion Alias, Z. Clark, A.G. R Medicine The effect of acute treatment of methyl parathion (MP) on the expression of BSP/GSH-agarose purified glutathione S-transferases (GSTs) in Drosophila melanogaster was investigated. Using 2-D gel analysis of the identified Epsilon-class, only DmGSTE6 (100%) and DmGSTE7 (72%) demonstrated significant increases in expression, suggesting the possibility that both may be involved in MP metabolism. A smaller percentage increase was also observed in DmGSTD1 (18%), a known DDT dehydrochlorinase, DmGSTE3 and DmGSTE9 and a putative Epsilon-class GST, CG16936, were shown not to be responsive to the challenge. Our finding demonstrates that not all member of the Epsilon-class GST, which are known for their role in insecticide resistance, are immediately responsive to this toxic challenge. (C) 2010 Elsevier Inc. All rights reserved. Academic Press Inc Elsevier Science 2010 Article PeerReviewed Alias, Z. and Clark, A.G. (2010) Adult drosophila melanogaster glutathione S-transferases: effects of acute treatment with methyl parathion. Pesticide Biochemistry and Physiology, 98 (1). pp. 94-98.
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
spellingShingle R Medicine
Alias, Z.
Clark, A.G.
Adult drosophila melanogaster glutathione S-transferases: effects of acute treatment with methyl parathion
description The effect of acute treatment of methyl parathion (MP) on the expression of BSP/GSH-agarose purified glutathione S-transferases (GSTs) in Drosophila melanogaster was investigated. Using 2-D gel analysis of the identified Epsilon-class, only DmGSTE6 (100%) and DmGSTE7 (72%) demonstrated significant increases in expression, suggesting the possibility that both may be involved in MP metabolism. A smaller percentage increase was also observed in DmGSTD1 (18%), a known DDT dehydrochlorinase, DmGSTE3 and DmGSTE9 and a putative Epsilon-class GST, CG16936, were shown not to be responsive to the challenge. Our finding demonstrates that not all member of the Epsilon-class GST, which are known for their role in insecticide resistance, are immediately responsive to this toxic challenge. (C) 2010 Elsevier Inc. All rights reserved.
format Article
author Alias, Z.
Clark, A.G.
author_facet Alias, Z.
Clark, A.G.
author_sort Alias, Z.
title Adult drosophila melanogaster glutathione S-transferases: effects of acute treatment with methyl parathion
title_short Adult drosophila melanogaster glutathione S-transferases: effects of acute treatment with methyl parathion
title_full Adult drosophila melanogaster glutathione S-transferases: effects of acute treatment with methyl parathion
title_fullStr Adult drosophila melanogaster glutathione S-transferases: effects of acute treatment with methyl parathion
title_full_unstemmed Adult drosophila melanogaster glutathione S-transferases: effects of acute treatment with methyl parathion
title_sort adult drosophila melanogaster glutathione s-transferases: effects of acute treatment with methyl parathion
publisher Academic Press Inc Elsevier Science
publishDate 2010
url http://eprints.um.edu.my/12502/
_version_ 1643689312758267904
score 13.211869

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