Cover Image

Exochelin production in Mycobacterium neoaurum.

Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most my...

Full description

Saved in:
Bibliographic Details
Main Author: Chan, K.
Format: Article
Published: MDPI 2009
Subjects:
RB Pathology
Online Access:http://eprints.um.edu.my/1081/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2662466/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.1081
record_format eprints
spelling my.um.eprints.10812017-07-08T05:42:28Z http://eprints.um.edu.my/1081/ Exochelin production in Mycobacterium neoaurum. Chan, K. RB Pathology Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under ironsufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 microg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated. MDPI 2009-01 Article PeerReviewed Chan, K. (2009) Exochelin production in Mycobacterium neoaurum. International Journal of Molecular Sciences, 10 (1). pp. 345-53. ISSN 1661-6596 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2662466/ 19333449
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic RB Pathology
spellingShingle RB Pathology
Chan, K.
Exochelin production in Mycobacterium neoaurum.
description Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under ironsufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 microg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated.
format Article
author Chan, K.
author_facet Chan, K.
author_sort Chan, K.
title Exochelin production in Mycobacterium neoaurum.
title_short Exochelin production in Mycobacterium neoaurum.
title_full Exochelin production in Mycobacterium neoaurum.
title_fullStr Exochelin production in Mycobacterium neoaurum.
title_full_unstemmed Exochelin production in Mycobacterium neoaurum.
title_sort exochelin production in mycobacterium neoaurum.
publisher MDPI
publishDate 2009
url http://eprints.um.edu.my/1081/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2662466/
_version_ 1643686673322606592
score 13.211869

Similar Items