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Plant regeneration and induction of coloured callus from henna (Lawsonia inermis syn. Lawsonia alba)

Tissue culture studies of Laivsonia inermis, syn. Lawsonia alba (henna) were carried out to induce multiple shoots, plant regeneration and coloured callus on MS media supplemented with various hormones. Various explants, such as sections of leaves, stems and roots, from 4-month-old aseptic seedlings...

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Main Authors: Rahiman, F.A., Taha, R.M.
Format: Article
Language:English
Published: 2011
Subjects:
QH301 Biology
Online Access:http://eprints.um.edu.my/10758/1/Plant_regeneration_and_induction_of_coloured_callus_from_henna_%28Lawsonia_inermis_syn._Lawsonia_alba%29.pdf
http://eprints.um.edu.my/10758/
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spelling my.um.eprints.107582014-10-15T01:01:51Z http://eprints.um.edu.my/10758/ Plant regeneration and induction of coloured callus from henna (Lawsonia inermis syn. Lawsonia alba) Rahiman, F.A. Taha, R.M. QH301 Biology Tissue culture studies of Laivsonia inermis, syn. Lawsonia alba (henna) were carried out to induce multiple shoots, plant regeneration and coloured callus on MS media supplemented with various hormones. Various explants, such as sections of leaves, stems and roots, from 4-month-old aseptic seedlings were used. Different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), napthalene acetic acid (NAA), benzyl aminopurine (BAP), kinetin, and indole acetic acid (IAA) hormones were used. The results showed that efficient regeneration system for henna plant in vitro could be achieved. Different coloured callus were obtained from henna tissue culture on MS supplemented with different combinations and concentrations of hormones 0.5 mg/L 2,4-D and 0.5 mg/L IAA, 2.0 mg/L NAA and 2.0 mg/L BAP, 0.5 mg/L NAA and 0.5 ing/L BAP, 1.5 mg/L NAA and 1.5 mg/L BAP, and also 0.5 mg/L IAA and 0.5 mg/L BAP, and thus production of important secondary metabolites in vitro is possible for this important species. Formation of embryogenic callus was observed during the plant morphogenesis in MS medium supplemented with combination of 0.5 mg/L IAA and 0.5 mg/L 2,4-D hormones which will enhance more production of in vitro plantlets and ultimately promote new propagules of this species. 2011 Article PeerReviewed application/pdf en http://eprints.um.edu.my/10758/1/Plant_regeneration_and_induction_of_coloured_callus_from_henna_%28Lawsonia_inermis_syn._Lawsonia_alba%29.pdf Rahiman, F.A. and Taha, R.M. (2011) Plant regeneration and induction of coloured callus from henna (Lawsonia inermis syn. Lawsonia alba). Journal of Food Agriculture & Environment, 9 (2). pp. 397-399. ISSN 1459-0255
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Rahiman, F.A.
Taha, R.M.
Plant regeneration and induction of coloured callus from henna (Lawsonia inermis syn. Lawsonia alba)
description Tissue culture studies of Laivsonia inermis, syn. Lawsonia alba (henna) were carried out to induce multiple shoots, plant regeneration and coloured callus on MS media supplemented with various hormones. Various explants, such as sections of leaves, stems and roots, from 4-month-old aseptic seedlings were used. Different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), napthalene acetic acid (NAA), benzyl aminopurine (BAP), kinetin, and indole acetic acid (IAA) hormones were used. The results showed that efficient regeneration system for henna plant in vitro could be achieved. Different coloured callus were obtained from henna tissue culture on MS supplemented with different combinations and concentrations of hormones 0.5 mg/L 2,4-D and 0.5 mg/L IAA, 2.0 mg/L NAA and 2.0 mg/L BAP, 0.5 mg/L NAA and 0.5 ing/L BAP, 1.5 mg/L NAA and 1.5 mg/L BAP, and also 0.5 mg/L IAA and 0.5 mg/L BAP, and thus production of important secondary metabolites in vitro is possible for this important species. Formation of embryogenic callus was observed during the plant morphogenesis in MS medium supplemented with combination of 0.5 mg/L IAA and 0.5 mg/L 2,4-D hormones which will enhance more production of in vitro plantlets and ultimately promote new propagules of this species.
format Article
author Rahiman, F.A.
Taha, R.M.
author_facet Rahiman, F.A.
Taha, R.M.
author_sort Rahiman, F.A.
title Plant regeneration and induction of coloured callus from henna (Lawsonia inermis syn. Lawsonia alba)
title_short Plant regeneration and induction of coloured callus from henna (Lawsonia inermis syn. Lawsonia alba)
title_full Plant regeneration and induction of coloured callus from henna (Lawsonia inermis syn. Lawsonia alba)
title_fullStr Plant regeneration and induction of coloured callus from henna (Lawsonia inermis syn. Lawsonia alba)
title_full_unstemmed Plant regeneration and induction of coloured callus from henna (Lawsonia inermis syn. Lawsonia alba)
title_sort plant regeneration and induction of coloured callus from henna (lawsonia inermis syn. lawsonia alba)
publishDate 2011
url http://eprints.um.edu.my/10758/1/Plant_regeneration_and_induction_of_coloured_callus_from_henna_%28Lawsonia_inermis_syn._Lawsonia_alba%29.pdf
http://eprints.um.edu.my/10758/
_version_ 1643688879344058368
score 13.211869

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