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HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from gymnema sylvestre methanolic extracts

Gymnemic acid (GA), a well known anti-diabetic compound has been detected in methanol extracts of intact leaves and in vitro callus cultures derived from leaf explants of Gymnema sylvestre. Callus biomass was developed in MS medium with optimum plant growth regulators (OPGRs) of 2,4-D (1.5 mg L-1) +...

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Main Authors: Ahmed, A.B.A., Rao, A.S., Rao, M.V., Taha, R.M.
Format: Article
Published: 2013
Subjects:
QH301 Biology
Online Access:http://eprints.um.edu.my/10737/
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spelling my.um.eprints.107372014-07-25T01:04:36Z http://eprints.um.edu.my/10737/ HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from gymnema sylvestre methanolic extracts Ahmed, A.B.A. Rao, A.S. Rao, M.V. Taha, R.M. QH301 Biology Gymnemic acid (GA), a well known anti-diabetic compound has been detected in methanol extracts of intact leaves and in vitro callus cultures derived from leaf explants of Gymnema sylvestre. Callus biomass was developed in MS medium with optimum plant growth regulators (OPGRs) of 2,4-D (1.5 mg L-1) + KN (0.5 mg L-1) under abiotic stress conditions at 45 days determined by growth curve analysis. GA detection and quantification were carried out using thin-layer chromatography (TLC), high-performance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), and gravimetric techniques. GA detection peak area and their absorption spectra were evaluated through HPTLC and HPLC with the standard GA. Quantification of GA had showed the linearity, accuracy, robustness and precision by HPLC. GA content was significantly higher in gravimetric method than HPLC. All these methods were found to be simple, accurate, selective and rapid and could be successfully applied for the determination of GA. It could have potential as a pharmaceutical drug for Type 1 diabetes mellitus (IDDM) and obesity. 2013 Article PeerReviewed Ahmed, A.B.A. and Rao, A.S. and Rao, M.V. and Taha, R.M. (2013) HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from gymnema sylvestre methanolic extracts. Acta Chromatographica, 25 (2). pp. 339-361. ISSN 1233-2356
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QH301 Biology
spellingShingle QH301 Biology
Ahmed, A.B.A.
Rao, A.S.
Rao, M.V.
Taha, R.M.
HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from gymnema sylvestre methanolic extracts
description Gymnemic acid (GA), a well known anti-diabetic compound has been detected in methanol extracts of intact leaves and in vitro callus cultures derived from leaf explants of Gymnema sylvestre. Callus biomass was developed in MS medium with optimum plant growth regulators (OPGRs) of 2,4-D (1.5 mg L-1) + KN (0.5 mg L-1) under abiotic stress conditions at 45 days determined by growth curve analysis. GA detection and quantification were carried out using thin-layer chromatography (TLC), high-performance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), and gravimetric techniques. GA detection peak area and their absorption spectra were evaluated through HPTLC and HPLC with the standard GA. Quantification of GA had showed the linearity, accuracy, robustness and precision by HPLC. GA content was significantly higher in gravimetric method than HPLC. All these methods were found to be simple, accurate, selective and rapid and could be successfully applied for the determination of GA. It could have potential as a pharmaceutical drug for Type 1 diabetes mellitus (IDDM) and obesity.
format Article
author Ahmed, A.B.A.
Rao, A.S.
Rao, M.V.
Taha, R.M.
author_facet Ahmed, A.B.A.
Rao, A.S.
Rao, M.V.
Taha, R.M.
author_sort Ahmed, A.B.A.
title HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from gymnema sylvestre methanolic extracts
title_short HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from gymnema sylvestre methanolic extracts
title_full HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from gymnema sylvestre methanolic extracts
title_fullStr HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from gymnema sylvestre methanolic extracts
title_full_unstemmed HPTLC/HPLC and gravimetric methodology for the identification and quantification of gymnemic acid from gymnema sylvestre methanolic extracts
title_sort hptlc/hplc and gravimetric methodology for the identification and quantification of gymnemic acid from gymnema sylvestre methanolic extracts
publishDate 2013
url http://eprints.um.edu.my/10737/
_version_ 1643688874315087872
score 13.211869

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